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EC number: 238-942-1 | CAS number: 14871-79-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- Experimental data of read across substances
- Justification for type of information:
- Data for the target chemical is summarized based on the closely related read across chemicals.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mention below
- Principles of method if other than guideline:
- WoE report was prepared based on short term toxicity of test chemical to aquatic invertebrates study.
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Details on test solutions:
- WOE2: Details on test solutions
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution from each metal salt was prepared in double Glass-distilled water. Serial dilutions were prepared from the respective stocks to the desired range. - Test organisms (species):
- other: Cypris subglobosa, aquatic crustacea and Daphnia magna were used
- Details on test organisms:
- WOE2: Details on test organisms
TEST ORGANISM
- Common name: Ostracod
- Strain: C. subglobosa Sowerby, 1840
- Source: fish ponds situated at Gheru Campus of IITR, Lucknow, India
- Method of breeding: Living specimens were taken to the laboratory and placed in 5-l glass beaker filled with filtered well water.
- Feeding during test: No
ACCLIMATION
- Acclimation period: 2-3 days
- Acclimation conditions (same as test or not): Test animals were acclimatized to laboratory conditions in environmental growth chamber at 21±1 ◦C for 2–3 days. Growth chamber was equipped with two 60W cool white fluorescent lights. Tests were carried under light: dark cycle of 12:12 h.
- Type and amount of food: Food consisted of a 1000mgl−1 suspension of dry fish food and yeast. The composition of fish food was as crude protein 52%, crude fiber 7%, crude fat 8% and moisture 8%.
WoE 4: Details on test organisms
TEST ORGANISM
- Common name: Water flea - Test type:
- other: WoE 2: Semi-static, WoE 3 and WoE 4: Static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
- Hardness:
- 245 mg/L CaCO3 (230–250)
- Test temperature:
- 21 (20–22) °C
- pH:
- 7.6 (7.4–7.7)
- Dissolved oxygen:
- 5.4 (5.1–6.1) mg/L
- Details on test conditions:
- WoE 2: Details on test conditions
TEST SYSTEM
- Test vessel: Petri- plate dishes
- Material, size, headspace, fill volume: 20 ml glass Petri- plate dishes were used
- Renewal rate of test solution (frequency/flow rate): After 24 hrs
- No. of organisms per vessel: 10 for each concentration
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per control (replicates) (Reference control): 9-12
- No. of vessels per vehicle control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
ALKALINITY: 400 390 TO 410 mg/L CaCO3
OTHER TEST CONDITIONS
- Photoperiod: 12 hours light and 12 hours dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobility of test organisms after the 24 hrs
TEST CONCENTRATIONS
- Range finding study: Final test concentrations were based on the results of preliminary range-finding short-term (48 h) tests. Ostracods were exposed for 48 h to logarithmic series of concentrations (7–10) of metals and reference toxicants. Nominal test concentrations in the range of 0.001, 0.01, 0.1, 1, 10, 100, 1000 and 10,000mg/l were selected for range-finding tests.
- Results used to determine the conditions for the definitive study: Endpoint used in the bioassay was immobility, i.e., the inhibition to swim within 15 second after gently agitation of the test container. Immobility implies the organism usually swim helplessly at the bottom of the container and use their limbs to crawled with difficulty. - Reference substance (positive control):
- yes
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 634 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95 % CI was 574 to 685 mg/l
- Duration:
- 48 h
- Dose descriptor:
- LC0
- Effect conc.:
- 940 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: WoE- 3
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 410 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- not specified
- Remarks on result:
- other: WoE - 4
- Reported statistics and error estimates:
- The 24 h and 48 h EC50 [effective concentration at which 50% immobilized response was recorded] values and their 95% confidence limits were calculated by the moving average-angle method.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- By applying weight of evidence approach the EC50 value of test chemical was determined to be in range from 410 mg/L to 940 mg/l after exposure of test chemical for 48 hrs. The range EC50 value indicates that the substance is likely to be nonhazardous to aquatic invertebrates and cannot be classified as per the CLP classification criteria.
- Executive summary:
Based on the experimental data for the test chemical, toxicity of test chemical were determine by observing the effect of chemical on the immobility of aquatic invertebrates. The studies are summarized as below:
Aim of this study was to determine the toxicity of test chemical on the mobility of aquatic invertebrate Cypris subglobosa. A stock solution from each metal salt was prepared in double glass-distilled water. Serial dilutions were prepared from the respective stocks to the desired range. Living specimens were taken to the laboratory and placed in 5 liter glass beaker filled with filtered well water. Test animals were acclimatized to laboratory conditions in environmental growth chamber at 21±1 °C for 2 to 3 days. Growth chamber was equipped with two 60W cool white fluorescent lights. Tests were carried under light and dark cycle of 12:12 h. 20 ml glass Petri- plate dishes were used in which 10 test organism added in each concentrations. Immobility implies the organism usually swim helplessly at the bottom of the container and use their limbs to crawl with difficulty. The 24 h and 48 h EC50 [effective concentration at which 50% immobilized response was recorded] values and their 95% confidence limits were calculated by the moving average-angle method. Based on the immobility of Cypris subglobosa by the test chemical exposure for 48 hrs, (EC50) was observed at 634 mg/l. Based on the EC50 value, chemical was consider to be nontoxic and not classified as per the CLP classification criteria.
Above study further supported by the second study from authoritative database. Principle of this study was to determine the effect of test chemical on aquatic invertebrate. The test chemical exposed with invertebrate for 48 hours. Based on the effects observation on the Crustaceans by the chemical exposure 48 hours, the LC50 was observed to be 410 mg/l. Thus based on the LC50 value, chemical was consider as nontoxic and can be consider to be not classified as per the CLP classification criteria.
Similar study was conducted to investigate the effect of test chemical on the growth of Daphnia magna. Study conducted according to the ISO 6341 15 "Water quality – Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea)". Test chemical exposed with the Daphnia magna for 48 hrs. After the exposure of test chemical with the Daphnia magna for 48 hrs, no effect (EC0) was observed on the mobility at the concentration 940 mg/l. Thus on the basis of above result, it can be concluded that the chemical was nontoxic and not classified as per the CLP classification criteria.
Thus based on the overall studies from various sources, it is concluded that the test chemical is nontoxic and not classified as per the CLP classification criteria.
Reference
Description of key information
By applying weight of evidence approach the EC50 value of test chemical was determined to be in range from 410 mg/L to >940 mg/l after exposure of test chemical for 48 hrs. The range EC50 value indicates that the substance is likely to be nonhazardous to aquatic invertebrates and cannot be classified as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 410 mg/L
Additional information
Based on the experimental data for the test chemical as well as read-across analogues which is extracted by using mechanistic approach and structurally similar to the test chemical, toxicity of test chemical were determine by observing the effect of chemical on the immobility of daphnia magna. The studies are summarized as below:
Aim of this study was to determine the toxicity of test chemical on the mobility of aquatic invertebrate Cypris subglobosa. A stock solution from each metal salt was prepared in double glass-distilled water. Serial dilutions were prepared from the respective stocks to the desired range. 0.2 ml of acetone were used as a vehicle. Living specimens were taken to the laboratory and placed in 5 liter glass beaker filled with filtered well water. Test animals were acclimatized to laboratory conditions in environmental growth chamber at 21±1 °C for 2 to 3 days. Growth chamber was equipped with two 60W cool white fluorescent lights. Tests were carried under light and dark cycle of 12:12 h. 20 ml glass Petri- plate dishes were used in which 10 test organism added in each concentrations. Immobility implies the organism usually swim helplessly at the bottom of the container and use their limbs to crawl with difficulty. The 24 h and 48 h EC50 [effective concentration at which 50% immobilized response was recorded] values and their 95% confidence limits were calculated by the moving average-angle method. Based on the immobility of Cypris subglobosa by the test chemical exposure for 48 hrs, (EC50) was observed at 634 mg/l. Based on the EC50 value, chemical was consider to be nontoxic and not classified as per the CLP classification criteria.
Above study further supported by the second study from authoritative database. Principle of this study was to determine the effect of test chemical on aquatic invertebrate. The test chemical exposed with invertebrate for 48 hours. Based on the effects observation on the Crustaceans by the chemical exposure 48 hours, the LC50 was observed to be 410 mg/l. Thus based on the LC50 value, chemical was consider as nontoxic and can be consider to be not classified as per the CLP classification criteria.
Similar study was conducted to investigate the effect of test chemical on the growth of Daphnia magna. Study conducted according to the ISO 6341 15 "Water quality – Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea)". Test chemical exposed with the Daphnia magna for 48 hrs. After the exposure of test chemical with the Daphnia magna for 48 hrs, no effect (EC0) was observed on the mobility at the concentration 940 mg/l. Thus on the basis of above result, it can be concluded that the chemical was nontoxic and not classified as per the CLP classification criteria.
Thus based on the overall studies from various sources, it is concluded that the test chemical is nontoxic and not classified as per the CLP classification criteria.
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