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EC number: 232-877-2 | CAS number: 9032-08-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 November 2005 - 02 November 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 227.9 mg enzyme concentrate dry matter/L
- Sampling method: Triplicate samples (5mL) were taken from the control and test concentration at 0 and 24 hours (fresh media) and at 24 and 48 hours (expired media) for analysis.
- Sample storage conditions before analysis: frozen at -20°C in the dark - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A sample of the test material weighing 1584 mg was dispersed directly in 1 litre of diluent water to give the nominal test concentration of 227.9 mg enzyme concentrate dry matter/L.
- Controls: medium - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus
- Source: Daphnia magna used in this study were cultured in-house and were obtained from a strain originating from the Institute National de Recherche Chimique Appliqué (IRChA), France
- Age at study initiation (mean and range, SD): less than 24 hours old - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- 21-22°C
- pH:
- 7.5-7.7
- Dissolved oxygen:
- 7.6-8.2
- Nominal and measured concentrations:
- A preliminary range finding test was conducted with nominal test concentrations of 1.04, 10.4, 103.6 and 227.9 mg enzyme concentrate dry matter/L.
Definitive study:
Nominal concentrations: 227.9 mg enzyme concentrate dry matter/L.
Measured concentrations: 264.2 mg enzyme concentrate dry matter/L. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL glass jars
- Type (delete if not applicable): loosely covered
- Material, size, headspace, fill volume: glass, 250 ml, fill volume: 100 mL
- Aeration: no (loosely covered jars)
- Renewal rate of test solution (frequency/flow rate): medium renewal after 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 medium
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hour light: 8 hour dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Observations of the condition of the test and control Daphnia were made following 24 and 48 hours exposure. Daphnia were considered to be immobile if they were unable to swim within approximately 15 seconds following gentle agitation of the test vessel.
TEST CONCENTRATIONS
Range finding study
- Test concentrations: 1.04, 10.4, 103.6 and 227.9 mg enzyme concentrate dry matter/L.
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 227.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: enzyme concentrate dry matter
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 227.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: enzyme concentrate dry matter
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: 0
- Validity criteria fulfilled:
- yes
- Conclusions:
- No immobilisation was observed following the exposure of Daphnia magna to the test substance at a concentration of 227.9 mg enzyme concentrate dry matter/L. Due to the low toxicity of the test material, the 48-hour EC50 (immobilisation) value of the substance was unable to be calculated. Therefore, the 48-hour EC50 value of the test substance to Daphnia magna was >227.9 mg enzyme concentrate dry matter/L (equivalent to 103.7 mg active enzyme protein/L).
The no-observed effect concentration (NOEC) was ≥ 227.9 mg enzyme concentrate dry matter/L (equivalent to ≥ 103.7 mg active enzyme protein/L). - Executive summary:
The acute toxicity of the test substance to Daphnia magna was assessed under semi-static exposure conditions. The study was conducted according to OECD and EC guidelines, and in compliance with GLP.
A group of twenty, first instar Daphnia magna, less than 24 hours old, were exposed to the test substance dispersed in Elendt M4 medium for 48 hours at a nominal concentration of 227.9 mg enzyme concentrate dry matter/L.
Verification of test concentrations was performed based on enzyme activity. The amount of measured enzyme (AGU/g) was then converted to mg enzyme concentrate dry matter/L in order to calculate the achieved measured concentrations. Average measured concentrations ranged from 119% of nominal at 0 hours (fresh), 115% at 24 hours (expired), 116% at 24 hours (fresh) and 114% at 48 hours (expired). These results confirmed that the nominal exposure concentration was achieved and maintained satisfactorily for the 24-hour duration between each medium renewal. Mean measured concentration was 264.2 mg enyzme concentrate dry matter/L. The results were reported in nominal concentrations, as a worse-case.
The numbers of immobilised daphnids were recorded for each test and control group after 24 and 48 hours.
No immobilisation was observed following the exposure of Daphnia magna to the test substance at a concentration of 227.9 mg enzyme concentrate dry matter/L. Due to the low toxicity of the test material, the 48-hour EC50 (immobilisation) value of the substance was unable to be calculated. Therefore, the 48-hour EC50 value of the test substance to Daphnia magna was >227.9 mg enzyme concentrate dry matter/L (equivalent to 103.7 mg active enzyme protein/L).
The no-observed effect concentration (NOEC) was ≥ 227.9 mg enzyme concentrate dry matter/L (equivalent to ≥ 103.7 mg active enzyme protein/L).
Reference
Description of key information
No immobilisation was observed following the exposure of Daphnia magna to the test substance at a concentration of 227.9 mg enzyme concentrate dry matter/L. Due to the low toxicity of the test material, the 48-hour EC50 (immobilisation) value of the substance was unable to be calculated. Therefore, the 48-hour EC50 value of the test substance to Daphnia magna was >227.9 mg enzyme concentrate dry matter/L (equivalent to 103.7 mg active enzyme protein/L).
The no-observed effect concentration (NOEC) was ≥ 227.9 mg enzyme concentrate dry matter/L (equivalent to ≥ 103.7 mg active enzyme protein/L).
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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