2-butoxyethyl benzoate

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-butoxyethyl benzoate

EC Number:

226-685-8

EC Name:

2-butoxyethyl benzoate

Cas Number:

5451-76-3

Molecular formula:

C13H18O3

IUPAC Name:

2-butoxyethyl benzoate

Test material form:

other: liquid

Details on test material:

2-Butoxyethyl benzoate, Lot # 201303443-19; Purity 99.2 %

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

Earthworms (Eisenia fetida) for the study were from Wildlife International cultures started with earthworms originally obtained from the University of Maryland Wye Research and Education Center, Queenstown, Maryland. The identity of the original culture was verified by the supplier. Earthworms were cultured in a mixture of moist peat moss with an appropriate amount of calcium carbonate, fed saturated alfalfa and/or cow manure, and held at a bedding temperature of 20 ± 5°C in continuous light.

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Test conditions

Test temperature:

18.4 - 20.0 C

pH:

7.5 - 7.6

Moisture:

33%

Nominal and measured concentrations:

Nominal concentrations: 62.5, 125, 250, 500, 1,000 mg/kg dry soil

Reference substance (positive control):

yes

Remarks:

chloroacetamide

Results and discussion

Effect concentrations

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

14-Day LC50: >1000 mg/kg dry soil
No Observed Effect Concentration (NOEC): 1000 mg/kg dry soil

Results with reference substance (positive control):

The 14-day LC50 estimation for earthworms exposed to the reference substance, chloroacetamide, in an artificial soil substrate was determined to be 19.7 mg a.i./kg dry soil with a 95% confidence interval of 17.9 and 22.0 mg a.i./kg dry soil. These results are consistent with those observed in previous studies and verify the adequacy and consistency of the methods used in this study.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

The 14-day LC50 value for earthworms exposed to 2-butoxyethyl benzoate in an artificial soil substrate was determined to be greater than 1000 mg/kg dry soil. The no-observed-effect-concentration (NOEC) was 1000 mg/kg dry soil, the highest concentration tested.