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EC number: 217-617-8 | CAS number: 1912-24-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 12 December 1988 to 20 April 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Atrazine
- EC Number:
- 217-617-8
- EC Name:
- Atrazine
- Cas Number:
- 1912-24-9
- Molecular formula:
- C8H14ClN5
- IUPAC Name:
- 6-chloro-N2-ethyl-N4-(propan-2-yl)-1,3,5-triazine-2,4-diamine
- Details on test material:
- Batch n. Lot 210200
Purity: 98.2%
Appaerance: white powder
Solubility in water: 30 mg/L
Storage: room temperature
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Duplicate samples (ca. 50 ml) were taken at 0h and 72 h.
Samples for analysis were taken at the start and at the end of the exposure.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- The nominal test concentrations (mg/L): 0.0037, 0.011, 0.033, 0.10, 0.30.
Calculated amounts of stock solution to produce the desired test concentrations were given into the water and were homogeneously distributed.
The stock solution has been prepared mixing 10 mg of atrazine with 4 mg of alkylphenol-polyglykol-ether and made up to 1000 ml with water. This solution was diluted to 0.303 mg/L with water.
Controls:
The blank was water
The vehicle: 0.12 mg alkylphenol-polyglykol-ether per liter water in the concentration used for the highest test concentration.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Test system: Scenedesmus subspicatus
Strain / Origin:276/20
Initial cell density: 1.2 * 10000 cells/ml
Culture: According to OECD-Guideline No. 201,1984
Preculture: 3 days under test conditions
Study design
- Test type:
- not specified
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- see salinity
- Test temperature:
- 23 ± 2°C, The temperature has been measured in continuous.
- pH:
- pH
Conc. Nominal mg/L 0 h 72 h
Blank 7.4 10.2
Vehicle 7.4 10.0
0.0037 7.4 10.1
0.011 7.4 10.1
0.033 7.4 9.5
0.10 7.4 7.9
0.30 7.4 7.8
The pH has been measured at the start and at the and of the exposure. - Dissolved oxygen:
- no data
- Salinity:
- Composition of water according to OECD-Guideline No. 201, Paris 1984
- Nominal and measured concentrations:
- The content of atrazine was found to vary between 91 and 102% of the nominal concentrations in the day 0 samples and between 78 and 93 % of the nominal concentrations in the day 3 samples.
- Details on test conditions:
- Exposure : 100 ml Erlenmeyer flasks. stoppered with aluminium caps, on lab-shaker, 50 ml test solution
Water : Composition according to: OECD-Guideline No. 201,1984
Temperature : 23 ± 2°C
Aeration : None
Light : Continuous illumination, cold white fluorescent light, 120 sE/m2sec ± 20% (approx. 8000 lux)
Duration : 72 hours
Each test concentration was tested in 3 replicates, the blank control in 6. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.043 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Remarks on result:
- other: none
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.011 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Details on results:
- EC50 (0 - 72h) calculated: 0.043 mg/L
EC50 (0 - 72h) graphically determined: 0.045 mg/L
NOEC (0 - 72h): 0.011 mg/L
Comparison of areas under the growth curve according to OECD-Guideline No. 201,1984.
The EC-50 values were calculated according to BERKSON, JASA 48 (1953), 569-599.
EC-values were graphically determined on gausso-logarithmic probabilitypaper.
The NOEC was calculated according to DUNNETT, C.W., JASA 50 (1955), 1096-1121, Biometrics 20 (1964), 482-491, and modified by MARCUS et al.,Biometrika 63 (1976), 655-660. - Results with reference substance (positive control):
- Not applicable
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
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