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EC number: 211-541-9 | CAS number: 660-68-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to the attached analogue justification provided in IUCLID Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 58.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Source: 6.1.3-1
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 56 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Source: Van Leeuwen, 1985
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 9 May - 16 June 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- according to the GLP standard by the Japanese Ministry of Environment
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature in the dark - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all test item concentrations were analyzed
- Vehicle:
- no
- Details on test solutions:
- According to guideline
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Age of parental stock: between 0 - 24 h
- Feeding during test: no feeding during test
ACCLIMATION
- Acclimation period: 14 d
- Type and amount of food: Chlorella vulugaris, 0.1 - 0.2 mgC/d
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20.4 - 20.7 °C (original test)
20.0 - 20.4 °C (supplemental test) - pH:
- 7.0 - 7.4 (control in the original test)
7.1 - 8.8 (test substance in the original test)
6.9 - 7.0 (control in the supplemental test)
6.9 - 10.7 (test substance in supplemental test) - Dissolved oxygen:
- 8.3 - 8.6 mg/L (control in the original test)
8.4 - 8.6 mg/L (test substance in the original test)
7.9 - 8.1 mg/L (control in the supplemental test)
8.2 - 8.9 mg/L (test substance in supplemental test) - Nominal and measured concentrations:
- Nominal concentration in the original test
0.79, 1.43, 2.57, 4.63, 8.33, and 15.0 mg/L
Geometric mean of measured concentration in the original test
0.57, 1.07, 1.89, 3.52, 6.41, and 12.6 mg/L
Nominal concentration in the supplemental test
27.0, 48.6, 87.5, and 157.5 mg/L
Geometric mean of measured concentration in the supplemental test
24.9, 45.3, 81.9, and 149.0 mg/L - Details on test conditions:
- TEST SYSTEM
- Fill volume: 100 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Culture medium different from test medium: no
- Intervals of water quality measurement: the content of the test item, pH and concentration of dissolved oxygen were measured at the beginning and at the end of the test
OTHER TEST CONDITIONS
- Photoperiod: light/dark 16/8 h
EFFECT PARAMETERS MEASURED: after 24 and 48 h the immobilised Daphnids were counted
TEST CONCENTRATIONS
- Range finding study: yes, without analytics, not further data in the report - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 58.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 24.9 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: no mortality observed
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50: 0.60 mg/L - Conclusions:
- The LC50 of the test substance ammounts 56 mg/L when tested under the conditions of this assay (OECD TG 202).
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1985
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- not specified
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
ACCLIMATION
- Type and amount of food: C. pyrenoidosa (1 x 10E8 cells/l) - Limit test:
- no
- Total exposure duration:
- 48 h
- Details on test conditions:
- TEST SYSTEM
- Test vessel: sealed vessels - Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 56 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI: 32 - 100 mg/L
- Reported statistics and error estimates:
- LC50 values an their 95% confidence intervals were calculated according to the Litchfield and Wilcoxon (1949) method
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The LC50 of diethylamine ammounts to 56 mg/L when tested under the conditions of this assay (OECD TG 202).
Referenceopen allclose all
Table 1. Measured concentrations of test substance
Original test
Nominal concentration (mg/L) |
Measured concentration (mg/L) |
Percent of nominal (%) |
|||
0 hour new |
48 hour old |
Geometric mean |
0 hour new |
48 hour old |
|
Control |
< 0.04 |
< 0.04 |
- |
- |
- |
0.79 |
0.58 |
0.56 |
0.57 |
73.4 |
70.9 |
1.43 |
1.08 |
1.06 |
1.07 |
75.5 |
74.1 |
2.57 |
1.90 |
1.88 |
1.89 |
73.9 |
73.2 |
4.63 |
3.39 |
3.65 |
3.52 |
73.2 |
78.8 |
8.33 |
6.37 |
6.45 |
6.41 |
76.5 |
77.4 |
15.0 |
12.7 |
12.6 |
12.6 |
84.7 |
84.0 |
Supplemental test
Nominal concentration (mg/L) |
Measured concentration (mg/L) |
Percent of nominal (%) |
|||
0 hour new |
48 hour old |
Geometric mean |
0 hour new |
48 hour old |
|
Control |
< 0.04 |
< 0.04 |
- |
- |
- |
27.0 |
25.4 |
24.4 |
24.9 |
94.1 |
90.4 |
48.6 |
45.9 |
44.8 |
45.3 |
94.4 |
92.2 |
87.5 |
83.3 |
80.5 |
81.9 |
95.2 |
92.0 |
157.5 |
151.1 |
146.9 |
149.0 |
95.9 |
93.3 |
New: freshly prepared test solutions
Old: test solutions after 48 hours exposure period
Table 2. Immobility of Daphnia magna exposed to test substance
Original test
Measured concentration (mg/L) |
Cumulative number of dead or immobilized daphnia (percent mortality or immobility) |
|
24 hour |
48 hour |
|
Control |
0 (0) |
0 (0) |
0.57 |
0 (0) |
0 (0) |
1.07 |
0 (0) |
0 (0) |
1.89 |
0 (0) |
0 (0) |
3.52 |
0 (0) |
0 (0) |
6.41 |
0 (0) |
0 (0) |
12.6 |
0 (0) |
0 (0) |
Supplemental test
Measured concentration (mg/L) |
Cumulative number of dead or immobilized daphnia (percent mortality or immobility) |
|
24 hour |
48 hour |
|
Control |
0 (0) |
0 (0) |
24.9 |
0 (0) |
0 (0) |
45.3 |
1 (5) |
10 (50) |
81.9 |
8 (40) |
12 (60) |
149.0 |
20 (100) |
20 (100) |
Description of key information
EC50 (48 h) = 56 mg/L (OECD 202) based on read across from CAS 109-89-7
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 56 mg/L
Additional information
Data on the toxicity of Diethylammonium chloride (CAS 660-68-4) to aquatic invertebrates are not available. Therefore read across to the analogues substance N-ethylethanamine (CAS 109-89-7) was conducted.
A reliable study (reference: 6.1.3-1) is available testing the toxicity of N-ethylethanamine (CAS 109-89-7) to aquatic invertebrates according to OECD 202 and GLP standards. The study was conducted in two steps. In the first part Daphnia magna, as the test organism was exposed for 48 h to test item concentrations of 0.79, 1.43, 2.57, 4.63, 8.33, and 15.0 mg/L (nominal). In a second step higher concentrations were tested (27.0, 48.6, 87.5, and 157.5 mg/L, nominal). Both tests were performed under static test conditions. An analytical monitoring was performed via HPLC. The recoveries of the substance were between 70 and 85% of the nominal values in the first test and > 90% in the second test. Therefore the results are expressed as geometric mean measured concentrations. The study resulted in an EC50 (48 h) of 58.4 mg/L (geom. mean meas.).
This result is in agreement with the findings of Van Leeuwen et al. (1985). The toxicity of N-ethylethanamine (CAS 109-89-7) to Daphnia magna was tested according to the guideline OECD 202. The study reported an EC50 (48 h) value of 56 mg/L (nominal). Since the publication is poorly documented it was used as the supporting study.
Similar effect levels to aquatic invertebrates are expected from the target substance Diethylammonium chloride (CAS 660-68-4).
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