3-chloropropyne

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-guideline study with unknown GLP status, limitations in design and reporting but outcome considered reliable and adequate for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38 412

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

3-chloropropyne

EC Number:

210-856-9

EC Name:

3-chloropropyne

Cas Number:

624-65-7

Molecular formula:

C3H3Cl

IUPAC Name:

3-chloroprop-1-yne

Details on test material:

Substance number: 83/124

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The product was added to the system as a 0.5% (w/v) aqueous solution.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Gold orfe
- Source: Fischzucht Eggers, D-2354 Hohenwestedt
- Received: 28-10-1982
- Length at study initiation (mean): 7.9 cm
- Weight at study initiation (mean): 4.2 g

ACCLIMATION
- Acclimation period: 41 weeks
- Acclimation conditions: same as test
- Type and amount of food: "Fukosalm" - Anschlussfutter 1; Fuko, Kraftfutterfabrik, D-7900 Ulm.
- Feeding frequency: ad libitum chloro

- Health during acclimation (any mortality observed): no mortality observed during 2-week period before beginning of the test. Fish received two medical treatments, once with 0.05 mg/L malachitgruenchlorid, once with 10 mg/L tetracyclinhydrochlorid.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

20±1°C

pH:

t=0: 7.4-7.7
t=96h: 7.7-7.8

Dissolved oxygen:

t=0: 5.3-7.6
t=96h: 7.4-8.0

Nominal and measured concentrations:

Nominal Concentration: 0, 6.81, 10, 14.7, 21.5, 31.6, 46.4, 68.1 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Vollglas aquarien
- Material, size, headspace, fill volume: glass tanks of 30 cm x 22 cm x 24 cm) filled with 10 L.
- No. of organisms per concentration: 10
- Biomass loading rate: 4.2 g fish/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water

OTHER TEST CONDITIONS
- Photoperiod: 16h/8h day/night

EFFECT PARAMETERS MEASURED:
- Mortality, apathy, exophtalmus, hyperreflexy, tumbling, discolouring,

Reference substance (positive control):

yes

Remarks:

Chloroacetamide

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 15 - < 22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No toxicologically significant symptoms were noted.

Results with reference substance (positive control):

LC50(48h): ±35 mg/L

Any other information on results incl. tables

Sublethal observations / clinical signs:

Mortality findings:

Nominal test concentration (mg/L)	Number of fish at t=0	Dead fish
		1h	4h	24h	48h	72h	96h
0	10	0	0	0	0	0	0
6.81	10	0	0	0	0	0	0
10.00	10	0	0	0	0	0	0
14.70	10	0	0	0	0	0	0
21.50	10	0	0	8	9	9	9
31.60	10	0	0	10	10	10	10
46.40	10	0	0	10	10	10	10
68.10	10	0	0	10	10	10	10
100.00	10	0	7	10	10	10	10