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EC number: 203-927-0 | CAS number: 112-00-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12th to 14th June 1984
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: "Methods for acute toxicity tests with fish, macroinvertebrates, and amphibians" U.S. EPA, 1975
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- reference material: x0296.01
Material description: clear, colorless liquid, 28% active ingredient - Analytical monitoring:
- not specified
- Details on sampling:
- Test concentrations: 0.39 ; 0.65 ; 1.1 ; 1.8 ; 3.0 ; 5.0 mg/L
Fifteen daphnids, < 24 hours old, were impartially distributed to each concentration (five daphnids per replicate) within - Vehicle:
- no
- Details on test solutions:
- A clear, colorless stock solution of 1.0 mg/mL was formulated by diluting 0.179 grams of X0296.01 with distilled water to volume in a 50-mL volumetric flask Test solution were formulated by adding the appropriate volumes of the stock solution to dilution water to total 1000 mL. Each solution was mixed on a magnetic strirrer for one minute and then divided into three beakers to provide replicate exposure treatments each containing 200 mL. The excess 400 mL of test solution was discarded. The test solution depth was 6.2 centimeters (cm) with a surface area of 33 cm2. Three control beakers containing the same dilution water and maintening under the same conditions as the exposure concentrations, but containing no X0296.01, were established.
Mortailities in replicate test solutions were recorded at 24 and 48 hours of exposure. Biological observations and observations of the physical characteristics of each replicate test solution were also made and recorded at 0, 24, and 48 hours. The pH's and dissolved oxygen concentrations were measured at 0 and 48 hours in one replicate vessel of each test concentration and the control. Temperature was measured in one replicate vessel of the control at 0, 24 and 48 hours of exposure. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The daphnids used in this toxicity test were obtained from laboratory stocks cultured at Springborn Bionomics, Inc., Wareham, Massachusetts. The culture water was prepared by fortifying well water based on the formula for hard water (U.S. EPA, 1975) and filtering it through carbon and Amberlite XAD-7 resin colums to remove any potential organic contaminants. this water had total hardness and alkalinity ranges as calcium carbonate (CaCO3) of 160-180 mg/L and 110-130 mg/L, respectively, a pH range of 7.9-8.3, a temperature of 20 +/- 1°C, a dissolved oxygen concentration of greater than 60% of saturation and a specific conductance range of 400-600 micromhos per centimeter (µmhos/cm). The daphnid culture area recieved a regulated photoperiod of 16 hours of light and eight hours darkness. Light at an intensity of 5-10 hectolux at the culture solutions' surface was provided by a combination of Sylvania Growlux(R) and Cool White(R) fluorescent bulbs. Daphnids were fed a solution of green algae (Ankistrodesmus sp.) and yeast suspension once daily. The air temperature was controlled to maintain the culture solutions at 20 +/- 1°C.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- not applicable
- Hardness:
- hardness and alkalinity ranges as calcium carbonate (CaCO3) of 160-180 mg/L and 110-130 mg/L, respectively.
- Test temperature:
- Test temperature: 20 +/- 1°C
- pH:
- pH range of 7.9-8.3
- Dissolved oxygen:
- Dissolved oxygen concentration of greater than 60% of saturation
- Salinity:
- NA
- Conductivity:
- specific conductance range of 400-600 micromhos per centimeter (µmhos/cm).
- Nominal and measured concentrations:
- Nominal concentrations: 0.39 ; 0.65 ; 1.1 ; 1.8 ; 3.0 ; 5.0 mg/L
Measured concentrations: results are reported as nominal concentrations of X0296.01 based on 28% active ingredient. - Details on test conditions:
- Test Vessels: 250 mL
Type: Open
Aeration: Slighty aerated
Number of organisms per vessels: 5
Number of vessels per concentrations (replicate): 3
Photoperiod: light/dark cycle 16/8 hours
Source/Preparation of dilution water: prepared as described in OECD 202 Test Guideline - Reference substance (positive control):
- yes
- Remarks:
- A sodium lauryl sulfate reference test was conducted with the daphnid test population from 15-17 May 1984
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 0.46 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- The concentrations tested, corresponding mortalities and observations made during the test. The 48-hour LC50 for Daphnia magna exposed to X0296.01 was calculated by nonlinear interpolation to be 0.46 mg/L with a 95% confidence interval of 0.39-0.65 mg/L estimated by binomial probability method. Table below in the section "Any other information on results incl. tables".
- Results with reference substance (positive control):
- A sodium lauryl sulfate reference test was conducted with the daphnid test population from 15-17 May 1984. The 48-hour LC50 and 95% confidence interval was 6.2(5.0-7.5) mg/L (Reference test log).
- Validity criteria fulfilled:
- yes
- Conclusions:
- Results arte reported as nominal concentrations of X0296.01 based on 28% active ingredient. The 48-hour LC50 was calculated by nonlinear interpolation to be 0.46 mg/L with a 95% confidence interval of 0.39-0.65 mg/L estimated by binomial probability.
- Executive summary:
Introduction:
The purpose of this study was to estimate the acute toxicity of X0296.01 to daphnids (Daphnia magna) under static conditions. A 48 -hour definitive test was conducted from 12 -14 June 1984, at the Aquatic Toxicology Laboratory of Springborn Bionomics, Inc., Wareham, Massachusetts. All raw data generated are stored at the above location.
Materials and methods:
Procedure used in this acute toxicity study followed those described in "Methods for acute toxicity tests with fish, macro-invertebrates, and amphibians" (U.S. EPA, 1975) and the protocol entitled "Static acute Frashwater Invertebrate Toxicity Study of X0296.01" (M.A. Lewis, Principal Investigator, 26 April 1984).
Results:
The concentrations tested, corresponding mortalities and observations made during the test. The 48 -hour LC50 for D. magna exposed to X0296.01 wazs calculated by nonlinear interpolation to be 0.46 mg/L with a 95% confidence interval of 0.39 -0.65 mg/L estimated by nominal probability method.
Reference
Concentrations tested, corresponding cumulative mortalities and observations made during the 48 -hour exposure of daphnids (Daphnia magna) to X0296.01.
Cumulative Mortality (%) | ||||||||
24 -hour | 48 -hour | |||||||
Nominal concentration (mg/L) | A | B | C | x | A | B | C | x |
5.0 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
3.0 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
1.8 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
1.1 | 60 | 80 | 60 | 67a | 100 | 100 | 100 | 100 |
0.65 | 0 | 0 | 0 | 0a | 100 | 100 | 100 | 100 |
0.39 | 0 | 0 | 0 | 0 | 20 | 20 | 20 | 20a |
control |
0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
(a): Sixty-seven percent or more of the surviving daphnids were lethargic and on the bottom of the test vessel.
Description of key information
Acute Toxicity of X0296.01 to Daphnia magna
Report #BW-84 -7 -1594
Study #1011 -0584 -6110 -110
Material: X0296.01
Description material: clear, colorless liquid, 28% active ingredient
Test date: 12 -14 June 1984
Species: Daphnia magna / Age: < 24 hours old / source: Bionomics culture facility
Dilution Water: fortified well water
Total hardness as CaCO3: 160mg/L
Total alkalinity as CaCO3: 120mg/L
pH: 8.3
Specific conductivity: 500µmhos/cm
Test temperature: 20 -21°C
Method of test material addition: stock solution, 1.0mg W0296.01/mL distilled water
Test concentrations: 0.39 ; 0.65 ; 1.1 ; 1.8 ; 3.0 ; 5.0 mg/L
Test vessels: 250mL
Number of organisms per vessels: 5
Number of vessels per concentrations (replicate): 3
photoperiod: light/dark cycle 16/8 hours
Source preparation of dilution water: prepared as described in OECD 202 test Guideline.
Test reference: A sodium lauryl sulfate reference test was conducted with the daphnid test population from 15 -17 May 1984. The 48 -hour LC50 and 95% confidence interval was 6.2(5.0 -7.5) mg/L (Reference test log).
Results: The concentrations tested, corresponding mortalities and observations made during the test. The 48 -hour LC50 for D. magna exposed to X0296.01 was calculated by nonlinear interpolation to be 0.46 mg/L with a 95% confidence interval of 0.39 -0.65 mg/L estimated by binimial probability method.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.46 mg/L
Additional information
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