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EC number: 203-298-2 | CAS number: 105-44-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04-06-2008 to 02-07-2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4-methylpentan-2-one oxime
- EC Number:
- 203-298-2
- EC Name:
- 4-methylpentan-2-one oxime
- Cas Number:
- 105-44-2
- Molecular formula:
- C6H13NO
- IUPAC Name:
- 4-methylpentan-2-one oxime
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 080301
- Expiration date of the lot/batch: not indicated
- Sample purity: 99.9%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: in the dark, at ambiant temperature, in the container in which it was received until required for testing
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge was obtained from Buckland Sewage Treatment Works, Newton Abbot, Devon, UK. This works treats sewage of predominantly domestic origin.
- Storage conditions: At the laboratory the activated sludge was kept aerated at room temperature and the pH maintained at approximately 7.
- Preparation of inoculum for exposure: The sludge was collected on the study start day. Before use the activated sludge was allowed to settle, the supernatant liquid decanted and the remaining sludge washed with test nutrient solution. Finally the supernatant was decanted and the total solids concentration determined on the remaining sludge.
- Pretreatment: no - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 400 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: The test medium was made up according to the OECD 302 B guideline (Ref 1) and contained approximately the following nutrients per litre of deionised water: 85 mg of KH2PO4, 217.5 mg of K2HPO4, 334 mg of Na2HPO4.2H2O, 5 mg of NH4Cl, 22.5 mg of MgSO4.7H2O, 36.4 mg of CaCl2.2H2O, 0.25 mg of FeCl3.6H2O and 0.40 mg of EDTA (disodium salt).
- Additional substrate: no
- Test temperature: 20 +/- 2°C
- pH: 6.5 - 8.0
- pH adjusted: yes. After being made up to volume (1000 ml), the pH of each treatment was measured and adjusted to between 6.5 and 8.0 using 2 M sodium hydroxide or 2 M hydrochloric acid as appropriate.
- Aeration of dilution water: The flasks were provided with gentle aeration and stirred (magnetic stirrer) for 28 days
- Continuous darkness: The laboratory was in darkness, except for when readings or samples were taken.
TEST SYSTEM
- Culturing apparatus: not indicated
- Number of culture flasks/concentration:
* blank control (inoculum only): 2 replicates
* positive control (inoculum + 300 mg/l aniline): 2 replicates
* exposure vessel (inoculum + 400 mg/l MIBKO): 2 replicates
- Method used to create aerobic conditions: The flasks were provided with gentle aeration and stirred with a magnetic stirrer.
- Measuring equipment: not indicated
- Test performed in closed vessels due to significant volatility of test substance: no
SAMPLING
- Sampling frequency: 3 hours after the start of the test and on days 1, 2, 7, 14, 21 and 28 (the positive control vessels were not sampled on Days 1 and 2).
- Sampling method: not indicated
- Sample storage before analysis: not indicated
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, two replicates with only inoculum
- Toxicity control: no
Reference substance
- Reference substance:
- aniline
Results and discussion
- Test performance:
- The results for the positive control show that a minimum of 70% aniline biodegradation had been achieved within 14 days, confirming the viability of the activated sludge.
The results for methyl isobutyl ketoxime show that the maximum biodegradation in the Zahn Wellens test of 98% was achieved after 28 days incubation.
The test substance appears to satisfy the criteria for inherent biodegradation
% Degradation
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 98
- Sampling time:
- 28 d
- Details on results:
- The results for methyl isobutyl ketoxime show that the maximum biodegradation in the Zahn Wellens test of 98% was achieved after 28 days incubation. The test substance appears to satisfy the criteria for inherent biodegradation
BOD5 / COD results
- Results with reference substance:
- The results for the positive control show that a minimum of 70% aniline biodegradation had been achieved within 14 days, confirming the viability of the activated sludge.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- A 28-d ready biodegradability test (OECD 302B, Zahn-Wellens/EMPA test) using unadapted activated sludge from a predominantly domestic waste water treatment plant indicated that methyl isobutyl ketoxine (MIBKO) is inherently biodegradable under the conditions of the test (initial concentration of 400 mg/l). The test substance showed 98% biodegradation on day 28. The results of the test can be considered reliable without restriction.
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