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EC number: 270-418-8 | CAS number: 68439-75-8 This substance is identified by SDA Substance Name: C12-C18 dialkyl methyl amine and SDA Reporting Number: 16-043-00.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: 1d: Reliable with restriction; guideline study but no analyses were performed to confirm the nominal test concentrations according to guideline.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Qualifier:
- according to guideline
- Guideline:
- other: Guideline 84/449/EWG
- GLP compliance:
- yes
Test material
- Reference substance name:
- Amines, di-C12-18-alkylmethyl
- EC Number:
- 270-418-8
- EC Name:
- Amines, di-C12-18-alkylmethyl
- Cas Number:
- 68439-75-8
- Molecular formula:
- C24 H51 N1 (representative formula for C12 alkyl chain)
- IUPAC Name:
- N-methyl-N-(C12-18)-alkyl(C12-18)-alkane-1-amine
- Test material form:
- liquid
- Details on test material:
- - Chemical name: N-methyl-N-(C12-18)-alkyl(C12-18)-alkane-1-amine
- EC number: 270-418-8
“Based on the qualitative and quantitative information on the composition, the sample used are representative of the boundary composition shared and agree by each registrant.”
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
not relevant
Sampling and analysis
- Analytical monitoring:
- not specified
- Details on sampling:
- no data
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Since the test substance is insoluble in water and since no better distribution was achieved using solvents and emulators, the test substance mixed with dilution water was homogenized using the Ultra-Turrax and ultrasonic bath. This solution was added to the test vessel while being stirred with at glass stirring rod. Finally, the contents of the test vessels were stirred for 2 hours with a KPG-stirrer and a glass stir rod.
At 2.2 and 10 mg/l, test substance deposits were observed.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- - Common name: Zebrafish
- Source: Hamilton-Buchanan/West Aquarium, Bad Lauterberg
- The fish were held in dilution water for at least 14 days before the beginning of the study.
- Mean body length of 10 fish = 3.0 cm
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- no data
- Test temperature:
- 22 ± 1°C
- pH:
- between 8.0 and 8.3
- Dissolved oxygen:
- > 80% oxygen saturation
- Salinity:
- no data
- Nominal and measured concentrations:
- Nominal concentrations: 2.2, 5, 10 and 50 mg/L
Measured concentrations: not stated - Details on test conditions:
- TEST SYSTEM
- Test vessel: 10-liter glass aquariums (30 x 22 x 24 cm)
- Aeration: no
- Feeding during test: no
- No. of organisms per vessel and control: 10
TEST MEDIUM / WATER PARAMETERS
- Dilution water was reconstituted water mixed to provide a composition following ISP/DIS 7346/l.
OTHER TEST CONDITIONS
- Photoperiod: 12-hour day/night cycles
- Light intensity: approximately 700 lux
- Feeding: twice daily - Reference substance (positive control):
- not required
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 6.15 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL = 4.5 to 8.3 mg/L
- Details on results:
- - Statistical methods: Probit analysis (Linder and Weber, confidence interval Fieller)
- After 2, 4, 24, 48, 72 and 96 hours, mortality and visible changes in appearance and behavior were noted. Dead fish were removed from the vessels. Fish were counted as dead when breathing was no longer seen and the fish no longer responded to a light mechanical stimulation.
- In all the test substance groups, impaired swimming, breathing as well as changed appearance and behavior were observed. Fish that died were pale or darkly colored and showed red gills and lockjaw.
- At the concentration 5 mg/L, 3 fish died after 96h. At the concentration 10 mg/L, 3 fish died after 48h and 9 died after 96h. All fish died after 48h at the concentration of 50mg/L.
- Additional endpoints included:
- 48-hour LC0 = 5 mg/l
- 96-hour LC0 = 2.2 mg/l
- 48-hour LC50 = > 10 mg/l and < 50 mg/l
- 48-hour LC100 = 50 mg/l
- 96-hour LC100 = 50 mg/l - Results with reference substance (positive control):
- not relevant
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Executive summary:
Acute toxicity of dicocomethylamine to Brachydanio rerio has been examinated through OECD Guideline 203, EU Method C.1 and GLP requirements.
Brachydanio rerio were exposed for 96 hours to dicocomethylamine
at nominal concentrations of 2.2, 5, 10 and 50 mg/L.
LC 50 - 96h was determined to be of 6.15 mg/L.
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