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EC number: 914-920-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study according to GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture: yes, Municipal activated sludge taken from a laboratory wastewater treatment plant fed with municipal sewage.
- Preparation of inoculum for exposure: The inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated during the night. 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/L dry substance in the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 180 min
- Test temperature:
- 20 ± 2 °C
- Dissolved oxygen:
- Oxygen concentration during aeration: > 2,5 mg/l
Oxygen concentration immediately before measurement: > 6,5 mg/l - Nominal and measured concentrations:
- 0, 62.5, 125, 250, 500, 1000 mg/l nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (nominal volume 250 ml)
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Synthetic medium: 8 ml/vessel 100-fold concentrated OECD medium - Reference substance (positive control):
- yes
- Remarks:
- dichlorophenol
- Duration:
- 180 min
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 180 min
- Dose descriptor:
- EC10
- Effect conc.:
- > 200 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- Al
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 180 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 180 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 200 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- Al
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- satisfactory
- Validity criteria fulfilled:
- yes
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1987
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- A clear guideline was followed, however little data on the substance identity and test organisms was presented so it is not 100% sure that the Al2(SO4)3, was the actual measured toxicant. Also no control group was used so effects, although a slim chance) might also be due to other conditions.
- Qualifier:
- according to guideline
- Guideline:
- other: Romanenko, V. I., Velichko, I. A.: Effect of chromium ions on vital activity of bacteria and algae. In: The biology of inland waters, No. 21 (in Russian). Nauka, Leningrad, 1974: 12. 8.
- Qualifier:
- according to guideline
- Guideline:
- other: Romanenko, V. I., Zhonson , T. D., Mikryakov, V. R.: Methods of utilizing heterotrophic assimilation of CO2 in determining the effect of pesticides on vital activity of bacteria. In: The biology of inland waters, 44. (In Russian). Nauka, Leningrad, 197
- Qualifier:
- according to guideline
- Guideline:
- other: Panasenkov, Yu. V.: Determination of the effect of sewage waters of a cellulose plant on natural microbial water coenoses according to absorption of 14-CO2 In: The biology of inland waters, 61. (In Russian) Nauka, Leningrad, 1983: 7.
- Principles of method if other than guideline:
- exposure time is 24h
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Details on sampling:
- After exposure and fixing with Lugol's solution, 5 filters
were obtained from each bottle. To remove the labelled carbonate adsorbed on the surface of
the bacterial cells and the ultrafilters, they were washed in 3 % HCI solution. Then the filters
were dried. After that, no sorption of labelled carbonate was observed on the filter and the
surface of the cells. - Vehicle:
- not specified
- Details on test solutions:
- The water taken from the reservoir is
distributed into 20-litre tanks into which aluminium sulphate is added in concentrations of
0.1, 1, 10 and 100 mI/L, respectively. - Test organisms (species):
- aerobic microorganisms
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
- Nominal and measured concentrations:
- nominal: 0.1, 1.0, 10, 100 mg/L
- Details on test conditions:
- The experiment is arranged in two parallels, i.e.,
there are two tanks of each concentration. After a fixed period (the experiment takes 24
hours), water samples are collected from the tanks for inoculation on nutrient media to
assess the amount off saprophytes (standard method) and to determine heterotrophic
assimilation and intensity of photosynthesis. The assessment of the amount of saprophytic
microflora is carried out on 10 Petri dishes for each concentration. - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Reported statistics and error estimates:
- According to these values and using V. 1. Romanenko's correlations
it was possible to calculate bacterial production. Statistical analysis of the obtained data
consisted in the calculation of the means and the value of the P > 0.95 confidence interval
using the methods of biological statistics. Statistical analyses of the values of radioactivity
of the bacteria have shown that the values of confidence intervals are negligible.
This justified the omission of a more detailed analysis of the obtained results. - Validity criteria fulfilled:
- not applicable
- Conclusions:
- The EC50 was determined to be 8 mg/L, the LOEC was determined to be 1 mg/L nominal. The usebalitlity of the data for risk assessment purposes is questionable due to the missing substance indentity data and the fact that no control group was used. It is therefore adviced to use this data only as supporting and not as keystudy.
- Executive summary:
The methods of determining the effect of chemical compounds on the processes of
microbial self-cleaning of waters are mainly based on the calculation of the number of indicator
microorganisms grown after being incubated on complex nutrient media following exposure of the
waters with the substances under study. These methods examine the reaction of specific
microorganisms under conditions rather different from those really existing in the water
reservoir. The author proposes to use the method of heterotrophic assimilation of CO2 for the
determination of the activity of natural bacterial associations in the study of the effect on
them of both multicomponent sewage waters of industrial plants and specific chemical compounds.
The determination of the values of bacterial productivity according to heterotrophic assimilation
of CO2 by natural microbial coenosis was used as the criterion of the effect of the toxicant..
Toxicity of the substance under study is evaluated according to its inhibitory effect on the
production of natural bacteriocoenosis (test object). Field of application: water toxicology,
supervision of toxicity of aqueous media, evaluation of the quality of water.
Standard experiment examining the effect of aluminium sulphate on natural bacterial
coenosis is considered an example. It has been demonstrated that the effect of aluminium
sulphate begins to be felt in concentrations starting from 1 mg/L. Statistical analysis
of results was carried out by calculating the mean value and confidence intervals (EC50 was determined to be 8 mg/L, nominal).
The investigations have shown that application of the method of heterotrophic assimilation
of CO2 for these purposes is feasible and of considerable interest with a view to its high
sensitivity and precision.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- weight of evidence
- Study period:
- 25 February 2010
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The study was conducted according to an appropriate OECD test guideline, and in compliance with GLP. The study is considered to be reliability 1 (reliable without restrictions); the read across of the result is considered to be reliability 2 (reliable with restrictions).
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The batch of 202028/A tested was a clear colourless liquid and was treated as 100% pure.
A stock solution of 0.5 g/l was prepared by adding 125.4 mg test substance to 250 ml of Milli-RO water (tap water purified by reverse osmosis; Millipore Corp., Bedford, Mass., USA). Thorough mixing (15 minutes) was used to accelerate dissolution and to ensure homogeneity. Volumes of the clear colourless stock solution, without flocks, corresponding to the test concentration were then added to the test media. A concentration of 100 mg/l was tested in duplicate. Optimal contact between the test substance and test medium was ensured applying continuous aeration and stirring during the 3-hour exposure period. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The sludge was coarsely sieved, washed and diluted with ISO-medium. A small amount of the sludge was weighed and dried overnight at ca. 105°C to determine the amount of suspended solids (4.0 g/l of sludge, as used for the test). The pH was 7.8 on the day of testing. The batch of sludge was used one day after collection; therefore 50 ml of synthetic sewage feed was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- Oxygen consumption was measured and recorded for approximately 10 minutes after the 3 hours exposure period.
- Test temperature:
- between 18.5 and 19.1°C
- pH:
- between 7.8 and 8.0
- Dissolved oxygen:
- Oxygen consumption:
Control series:66-68 mg O2/l/h
Test substance series: 63-66 mg O2/l/h
The oxygen concentration at the start of measurements was at least 7.2 mg O2/l - Nominal and measured concentrations:
- Nominal concentration: 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: All glass, approximately 300 ml oxygen bottles (measuring) and
1 litre test bottles(exposure)
- Aeration:During exposure with clean, oil-free air
- No. of vessels per concentration (replicates):2
- No. of vessels per control (replicates):2/ series
- Biomass loading rate:1.6 g/l suspended solids in final test mixture
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap-water purified by reverse osmosis (Milli-RO water)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The respiration rate from each vessel, in mg O2/l/hr, was calculated from the linear part of the respiration curve, which was generally between 2.5 and 6.5 mg O2/l.
TEST CONCENTRATIONS
- Test concentrations: limit study at 100 mg/l - Reference substance (positive control):
- yes
- Remarks:
- (3,5-dichlorophenol)
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 4.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- Al
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 4.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- Al
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- No significant inhibition of respiration rate of the sludge was recorded at 100 mg 202028/A per litre. The duplicate measurement confirmed the result of the first measurement. Therefore, no further testing was needed. Hence, the EC50 of 202028/A exceeded 100 mg/l (based on nominal concentrations).
- Results with reference substance (positive control):
- Results with reference substance valid: Yes
The inhibitory effect of 3,5-dichlorophenol on aerobic waste water (activated sludge) bacteria increased with increased with increasing concentration , ranging from a 29 % inhibition at 5.0 mg/l to 84 % at 30 mg/l. Relevant effects levels: The EC50 of 3,5-dichlorophenol was: 9.0 mg/l - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this present test, 202028/A was not toxic to waste water (activated sludge) bacteria at 100 mg/l. The 3-hour EC50 of 202028/A exceeded 100 mg/l (based on nominal concentrations).
Based on aluminum element the EC50 and EC10 are greater than 4.4 mg/l (total Al)
Referenceopen allclose all
Results of our studies demonstrate that the concentrations of 0.1 and 1 mg/L have no effect on the microorganisms while the concentration of 10 mg/L reduces the number of saprophytes by 60 %. It can be seen that even a concentration
of 1 mg/L of aluminium sulphate has some influence on the fixation of labelled carbonic acid by microorganisms. Further increase in the concentration of the toxicant leads to a decrease in CO2 absorption. On the basis of results
of the experiment, it can be stated that the concentration of aluminium sulphate which has no effect on natural microcoenosis (inactive concentration) amounts to 1 mg/L. The threshold concentration of the toxicant can be found within the interval between 1 and 10 mg/L.
At the mentioned concentration, heterotrophic fixation of 14-CO2 by microorganisms
decreased by 50 % and the number of saprophytes decreased by 60 %.
This confirms once again the possibility of using the radiocarbon method of estimating
bacterial production in water toxicology. The effect of the concentration of 100 mg/L on the microbial coenosis is
so much greater that it causes its destruction.
Description of key information
Key value for chemical safety assessment
Additional information
One activated sludge respiration inhibition test according to OECD 209 is available with aluminum chloride according to standard guideline (Schwarz, 2008). No effects were seen up to the highest test concentration of 1000 mg/l test material. The test substance contained 20 g Al/100 g and the test concentrations can be converted to mg/l Al. 1000 mg/l test material is equal to 200 mg/l Al. A second activated sludge respiration inhibition test is available for aluminum chloride hydroxide sulfate according to standard guideline (Desmares-Koopmans, 2010). It was performed as limit test at 100 mg/l test material. The test substance contained 4.4% Al and the test concentrations can be converted to mg/l Al. 100 mg/l test material is equal to 4.4 mg/l Al. Both tests available did not show any effect to microorganisms.
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