Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 610-813-8 | CAS number: 52250-47-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 day
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Ethyl-(3-hydroxypropyl)propanedioic acid
- EC Number:
- 610-813-8
- Cas Number:
- 52250-47-2
- Molecular formula:
- C8H14O5
- IUPAC Name:
- Ethyl-(3-hydroxypropyl)propanedioic acid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Duration of test (contact time):
- 28 d
- Details on study design:
- The amount of oxygen consumed by the microorganisms when metabolising the test item is called Biochemical Oxygen Demand (BOD). The BOD of test item is determined by the manometric respiratory - OxiTop® system. The principle of the determination of BOD by OxiTop® - system is based on the measurement of the consumption of oxygen by a pressure sensor in the top of the tightly closed test vessels. Test vessels with the test item solution and mixed bacterial culture, so called inoculum, are under continuous stirring in an incubator at 21 ± 1 °C for 28 days. The carbon dioxide which is evolved during the biodegradation is absorbed by sodium hydroxide granulates. The reduced amount of oxygen causes definite pressure changes in the test vessels which are measured by the apparatus. The amount of oxygen taken up by microorganisms during biodegradation of the test item is expressed as a percentage of theoretical oxygen demand (ThOD). The percentage of biodegradability of the test item is calculated on the base of quotient of 28-day biochemical oxygen demand (BOD28) and theoretical oxygen demand. The pass level for the biodegradability is 60% of ThOD for Manometric Respirometry method. The pass value has to be reached in 10 days (10-d) window within the 28-day period of the test. The 10-d window begins when the degree of biodegradation has reached 10% ThOD and must end before day 28 of the test. Biodegradability classification of the test item is made according to OECD 301 guideline, which says that chemicals which reach the pass level up to 28-d period are deemed to be readily biodegradable.
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
- Test performance:
- Preparation of inoculum test culture: Before the test 300 ml stock culture was filtered through a filter with 0.2 mm pore size to remove the bigger insoluble particles and then the dry substance content of the filtrate was determined. The measured value was 4.94 g/L. For preparing the inoculum culture 159.5 ml mineral medium was added to 40.5 ml filtered stock culture. The calculated dry mass of inoculum culture was 1 g/L.
Preparation of test vessels: Following test vessels were prepared and analysed for the study:
- Test item (in two replicates)
- Blank control (in two replicates)
- Reference solution (positive control)
- Reference and test item solution (toxicity control)
- Sterilized solution of test item (abiotic control)
Measurement of Biological Oxygen Demand values: The BOD values of the test vessels were collected and stored by measuring heads. The data were read out from the heads by the controller. The controller shows directly the BOD concentrations in mg/L. The daily BOD values were documented (see table below). Measured BOD values of blank control The maximum measured BOD value of the blank controls was 16.9 mg/L which is less than 60 mg/L so the used inoculum was proper for the test.
Measurement of pH: At the beginning and at the end of the test the pH was measured. The pH of test solutions was not adjusted at the beginning of the test.
The following table shows the measured pH values at the beginning and at the end of the test: A slightly elevated level of pH was observed throughout the incubation period.
Measurement of temperature: The temperature inside the incubator at the test vessels was measured and registered continuously by Extech SD200 thermometer and data logger. Throughout the test the temperature was nearly constant.
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 0
- Sampling time:
- 28 d
- Remarks on result:
- other: mean of two replicates
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- According to OECD guideline the test item should be classified as readily biodegradable if the percentage biodegradation reaches 60% of theoretical oxygen demand within 28 day period in a 10-day window. Based on ready biodegradability testing results the Ethylhydroxypropyl malonic acid is not readily biodegradable, because the biodegradability is 0%.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.