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Diss Factsheets
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EC number: 473-370-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 October 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- yes
- Remarks:
- water
- Details on test solutions:
- A 0.5 g/L solution was prepared by adding 125.0 mg to 250 mL water (purified by reverse osmosis). After thorough mixing, the solution was treated by ultrasonication (10 min) and magnetic stirring (32 min). This resulted in a clear and colourless solution that was added to the medium.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
Municipal Sewage Treatment Plant Waterschap De Maaskant, 's-Hertogenbosch
- Preparation of inoculum for exposure: sieved, washed and diluted with ISO-medium stored overnight (fed with 50 mg/L synthetic sewage feed) with aeration at 18 ˚C.
- Pretreatment: none
- Initial biomass concentration: SS 3.7 g/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- oxygen consumption measured over a 10 min period
- Test temperature:
- 18.0-18.8 ˚C
- pH:
- sludge: 7.8
test vessels: 8.2 - Dissolved oxygen:
- constant aeration during exposure
- Nominal and measured concentrations:
- 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1L glass vessel (300 mL oxygen bottles)
- Aeration: yes
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2 (one analysed before and one after the test substance)
- Sludge concentration (weight of dry solids per volume): 3.7 g/L SS
- Nutrients provided for bacteria: 16 mL synthetic sewage feed according to the guideline
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: adjusted ISO-medium according to the guideline
OTHER TEST CONDITIONS
- Adjustment of pH: none
EFFECT PARAMETERS MEASURED: oxygen consumption - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- see attachment
The difference between controls was 10% (< 15%) - Results with reference substance (positive control):
- 3,5-dichlorophenol as tested in the same test showed respiration inhibition of 49% at 5 mg/L and 83% at 30 mg/L. Therefore the EC50 is between the ranges of 5 and 30 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 for inhibtion of respiration is > 100 mg/L
- Executive summary:
In a respiration inhibition test the substance showed slight inhibition of the oxygen consumption of sludge (10 and 14% in duplicates) compared to controls. The EC50 is > 100 mg/L.
Reference
Description of key information
In a respiration inhibition test the substance showed slight inhibition of the oxygen consumption of sludge (10 and 14% in duplicates) compared to controls. The EC50 is > 100 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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