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EC number: 284-664-9 | CAS number: 84961-74-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 16 July 2012 - 17 October 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., compds. with 2-propanamine
- EC Number:
- 284-664-9
- EC Name:
- Benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., compds. with 2-propanamine
- Cas Number:
- 84961-74-0
- Molecular formula:
- N/A
- IUPAC Name:
- Benzenesulfonic acid, 4-C10-13-sec-alkyl derivs.-, compd. with 2-propanamine (1:1)
- Test material form:
- liquid: viscous
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken from the control and each test group (replicates R1 - R4 pooled) at 0 and 48 hours for quantitative analysis. All samples were stored at approximately -20°C prior to analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- An amount of test item (3300 mg) was dispersed in 11 liters of reconstituted water with the aid of propellor stirring at approximately 1500 rpm for 24 hours. After 24 hours the stirring was stopped and any undissolved test item removed by filtration through a 0.2µm Sartorius Sartopore filter (first approximate 1 liter discarded in order to pre-condition the filter) to give a 100% v/v saturated solution of the test item. A series of dilutions was made from the 100% v/v saturated solution test concentration to give further test concentrations of 56, 32, 18, 10, 5.6, 3.2, 1.8 and 1.0% v/v saturated solution
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: not reported
- Source: in-house laboratory culture
- Age at study initiation (mean and range, SD): <24 hours old
- Method of breeding: Adult Daphnia were maintained in 150 mL glass beakers containing Elendt M7 medium in a temperature controlled room at approximately 20°C. The lighting cycle was controlled to give 16 hours light and 8 hours darkness cycle with 20 minutes dawn to dusk transition periods. Each culture was fed daily with a mixture of algal suspension (Desmodesmus subspicatus) and Tetramin flake food suspension. Culture conditions ensured that reproduction was by parthenogenesis. Gravid adults were isolated the day before initiation of the test, such that the young daphnids produced overnight were less than 24 hours
- Feeding during test: None
ACCLIMATION
- Acclimation period: not necessary, conditions in culture are equal to test conditions
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 250 mg/L as CaCO3
- Test temperature:
- 20-21°C
- pH:
- start: 7.9 - 8.0
after 48 hours: 7.1 - 8.0 - Dissolved oxygen:
- start: 8.8 - 9.2 mg O2/L
after 48 hours: 8.7 - 8.8 mg O2/L - Nominal and measured concentrations:
- Nominal:
Control, 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100% saturated solution
Measured:
Control, 0.71, 1.3, 2.5, 5.0, 9.1, 13, 28, 44 and 86 mg test material/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:250 mL glass jars
- Type (delete if not applicable): covered to reduce evaporation
- Material, size, headspace, fill volume:250 mL glass jars filled to 200 mL
- Aeration: no
- Renewal rate of test solution (frequency/flow rate):static (no renewal)
- No. of organisms per vessel:5
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates):4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water prepared according to OECD 202 guideline
- Conductivity: <5 µS/cm
- Culture medium different from test medium: culure medium was Elendt M7
- Intervals of water quality measurement:pH and dissolved oxygen concentration were measured at the start and end of the test (t = 0 and t = 48 hours)
OTHER TEST CONDITIONS
- Adjustment of pH: not reported
- Photoperiod: 16 hours light and 8 hours darkness cycle with 20 minutes dawn to dusk transition periods
- Light intensity: 354 - 383 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation at 24h and 48h after start of exposure
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study
- Test concentrations:0.10, 1.0, 10 and 100% saturated solution of test material
- Results used to determine the conditions for the definitive study:0% immobilization at 0.10 and 1.0%, 80% immobilization at 10% and 100% immobilization at 100% saturated solution of test material - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 7.1 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 3.7-12 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.5 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 5 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: not reported
- Observations on body length and weight: not reported
- Other biological observations: not reported
- Mortality of control: 0%
- Other adverse effects control: none reported
- Abnormal responses: none reported
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no. Nominal values were expressed as percentage of a saturated solution. Measured concentrations in the range of 90% to 115% of the 0-hour measured concentrations were obtained at 48 hours, hence it was considered justifiable to base the results on the 0-hour measured test concentrations only.
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- - Results with reference substance valid? yes
48-h EC50 (immobilization) was 0.45 mg potassium dichromate/L (95% CL 0.42 - 0.48 mg/L).
The 48-h NOEC was found to be 0.32 mg/L.
These results are within the normal range for this reference item. - Reported statistics and error estimates:
- The EC50 values and associated confidence limits at 24 and 48 hours and the slope of the response curve and its standard error were calculated by the maximum-likelihood probit method (Finney, 1971) using the ToxCalc computer software package (ToxCalc, 1999).
Any other information on results incl. tables
Table 1. Cumulative immobilization of Daphnia magna exposed to 97% benzenesulfonic acid, 4-C10-13-sec-alkyl derivs.-, compd. with 2-propanamine (1:1)
0-hour measured test concentration (mg/L)* |
Cumulative immobilizedDaphnia (initial population: 5 per replicate) |
|||||||||||
24 hours |
48 hours |
|||||||||||
R1 |
R2 |
R3 |
R4 |
Total |
% |
R1 |
R2 |
R3 |
R4 |
Total |
% |
|
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.71 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1.3 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
1** |
5 |
2.5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
5.0 |
1 |
0 |
2 |
0 |
3 |
15 |
3 |
0 |
1 |
2 |
6 |
30 |
9.1 |
2 |
2 |
0 |
0 |
4 |
20 |
3 |
2 |
0 |
3 |
8 |
40 |
13 |
5 |
5 |
5 |
5 |
20 |
100 |
5 |
5 |
5 |
5 |
20 |
100 |
28 |
5 |
5 |
5 |
5 |
20 |
100 |
5 |
5 |
5 |
5 |
20 |
100 |
44 |
5 |
5 |
5 |
5 |
20 |
100 |
5 |
5 |
5 |
5 |
20 |
100 |
86 |
5 |
5 |
5 |
5 |
20 |
100 |
5 |
5 |
5 |
5 |
20 |
100 |
*Note that these concentrations are not corrected for purity (purity test material: 97% benzenesulfonic acid, 4-C10-13-sec-alkyl derivs.-, compd. with 2-propanamine (1:1)).
**Single immobilized daphnid considered to be due to natural causes rather than a true toxic effect given that less than 10% immobilization occurred.
Analytical results:
Measured concentrations in the range of 90% to 115% of the 0-hour measured concentration were obtained at 48 hours and hence it was considered justifiable to base the results on the 0-hour measured test concentrations only.
The 24-hour EC50 based on immobilisation was 9.0 mg test material/L with 95% CL of 6.2 - 12 mg/L.
The slopes and their standard errors of the response curves at 24 and 48 hours were 6.1 (SE = 1.8) and 3.7 (SE = 1.1) respectively.Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hour EC50 of benzenesulfonic acid, 4-C10-13-sec-alkyl derivs.-, compd. with 2-propanamine (1:1) to Daphnia magna was 7.1 mg/L.
- Executive summary:
The acute toxicity of benzenesulfonic acid, 4-C10-13-sec-alkyl derivs.-, compd. with 2-propanamine (1:1) (purity 97%)
to the fresh-water crustacean Daphnia magna was determined as decribed in the OECD 202 guideline and according to Good Laboratory Practice.The test was carried out with 4 x 5 daphnias for each concentration and control, and the test solutions were not replaced.
The exposure duration was 48 hours.
The concentrations tested were 0, 0.71, 1.3, 2.5, 5.0, 9.1, 13, 28, 44 and 86 mg/L and results were based on measured initial concentations.
All validity criteria were achieved and the results for the positive control were within the normal range, so the test is considered valid. The 48 h EC50 value based on mobility was 7.1 mg benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., compds. with 2-propanamine (1:1)/L, with 95% CL of 3.7 to 12 mg/L. This toxicity study is classified as acceptable and satisfies the guideline requirement for the OECD 202 method (Daphnia acute immobilisation test).
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