Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 267-400-7 | CAS number: 67859-51-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2021-Jul-07 to 2021--Nov-12, with the definitive exposure phase of the 2nd experiment from 2021-Sep-22 to 2021-Oct-13
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Version / remarks:
- 2012
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Name of test substance: EDTA-ZnK2
Test substance No.: 20/0482-1
Batch identification: DC-C 11530
CAS No.: 14689-29-3
Purity: 90.4 % (see Certificate of Analysis)
Identity: Confirmed (see Certificate of Analysis)
Homogeneity: given
Storage stability: Expiry date: Jun 2022
The stability under storage conditions over the exposure period was guaranteed by the sponsor, and the sponsor holds this responsibility.
Chemical Name: Dipotassium [[N,N'-ethylenebis[N-(carboxylatomethyl) glycinato]] (4-)-N,N',O,O',ON,ON']zincate(2-)
Synonyms: Ethylenediaminetetraacetic acid, Zinc dipotassium complex
Appearance
- physical state: solid
- color: white - Analytical monitoring:
- yes
- Remarks:
- Method of analysis: Mass spectrometry coupled to an HPLC-system
- Details on sampling:
- The concentration of the test substance was determined in one representative test interval per week in each freshly prepared test solution (test vessels without daphnids) and in the 48-h or 72-h old test solutions before renewal (combined sample from test vessels with daphnids).
Additionally, samples are collected and analyzed from the 48-hour or 72-hour old abiotic replicate (no Daphnia or food algae) per test group.
The samples were taken from the combined test solution from all test group replicates using a glass pipette and were transported to the analytical laboratory in glass ampoules.
For each concentration, two retained samples were taken at each time point. Retained samples are stored at the Laboratory ECO in a freezer (at approx. –20 °C). Analysis of these samples were performed in case of equivocal analytical results with the original samples or after loss of/ damage to original samples after agreement by the Study Director. Retained samples were described by the suffix “b” and “c” in the report. Following finalization of the report, all analytical samples, including retained samples, will be discarded. - Vehicle:
- no
- Details on test solutions:
- 0 (control), 1.56, 3.13, 6.25, 12.5, 25 mg/L as nominal concentrations based on test substance mass without correction for purity were prepared.
The stability of the test substance as a solution in test media and under testing conditions was determined by concentration control analysis.
The stock test solutions (25 mg/L) were prepared by dissolving 50 mg of test substance in 2-L of test medium and stirring for approximately 10 min at ambient temperature. The lower test concentrations were prepared by dilution of this stock solution. Before the dilution was performed, the stock solution was checked for complete dissolution of the test substance.
The actual mass of test substance weighed to prepare each stock solution is given below.
Day: 0 2 5 7 9 12 14 16 19
Test substance (mg) 50.80 50.38 50.88 50.06 50.69 49.97 50.17 50.55 50.56
pH values
Day: 0 2 5 7 9 12 14 16 19
0 mg/L 7.9 8.0 7.9 7.8 7.7 7.8 7.8 7.9 7.8
25 mg/L 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8 7.8
Dilutions scheme
Nominal conc. [mg/L] Volume of stock solution [mL] Final Volume of test media [mL]
0 (control) - 1000
1.56 63 1000
3.13 125 1000
6.25 250 1000
12.5 500 1000
The test solutions were visibly colorless and clear after preparation.
Reason for the selection of the test concentrations:
According to the test guidelines, at least 5 concentrations in a geometric series with a separation factor ≤3.2 (OECD [Ref. 2]) should be used.
In a preliminary test (experimental conduct in accordance with GLP but without a GLP status), after 14 days exposure to the test substance, no significant effect on reproduction was observed at 12 mg/L. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Test species : Daphnia magna STRAUS
Reason for selection of the test species:
Recommended species in the test guidelines.
Origin :
The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978.
From this date on this clone was cultured and bred continuously in the Ecotoxicology Laboratory of Experimental Toxicology and Ecology, BASF SE, Ludwigshafen Germany. - Test type:
- semi-static
- Water media type:
- freshwater
- Remarks:
- Elendt M4, according to OECD 211, Annex 2 is used. A synthetic fresh water (Elendt M4) is used as media for culture and test purposes. For the composition of this M4 medium see OECD 211.
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- Hardness was measured in the freshly prepared test solution in an additional replicate of each concentration for one interval per week and in the old test solution from the combined replicates of each test group for one interval per week.
- Test temperature:
- 19.4-20.3 °C.
The temperature was in a range of ±1°C. - pH:
- For details see section "Any other information on results incl. tables" below.
- Dissolved oxygen:
- The dissolved oxygen concentration was above 3 mg/L.
For details see section "Any other information on results incl. tables" below. - Nominal and measured concentrations:
- 0 (control), 1.56, 3.13, 6.25, 12.5, 25 mg/L as nominal concentrations based on test substance mass without correction for purity were prepared.
The test solutions were visibly homogeneous.
Method of analysis: Mass spectrometry coupled to an HPLC-system.
Limit of quantification (LOQ): 0.1 mg/L.
Recovery efficiency: 87-106 %.
All measured concentrations deviated less than 20% from the nominal concentration.
Thus,the test substance was stable in solution under test conditions and nominal concentrations are an accurate representation of exposure levels maintained throughout the test period.
Following recommendations in OECD 211 the results should be evaluated based on the nominal concentrations.
Additionally, samples were analyzed from the abiotic replicate of each test concentration and the control at the end of the renewal interval. All abiotic replicates were within ±20% of the nominal concentrations. These samples were analyzed to assist in the interpretation of results in the event that food algae or Daphnia over the exposure period interfered with the test substance analyses. Since the biotic test replicate samples were all within ±20% of the nominal, the additional analyses of abiotic replicates have no influence on the interpretation of this study. - Details on test conditions:
- At start of exposure 1 neonate (<24 h old) Daphnia magna was distributed impartially into each of the 10 replicate test vessels per test group. The test vessels were maintained and daphnids fed. Parent mortality, abnormal effects, and numbers of live and dead offspring were assessed every day throughout the experiment. Reproductive success was measured by counting and discarding
the offspring produced by each parent for the duration of the study. Separating neonates from
adults was accomplished by gently removing adult daphnids from each chamber by means of a wide bore pipet and pouring remaining water and young daphnids through a fine mesh screen. The young collected on the screen were placed in a petri plate and counted on a light table before being discarded.
On non-renewal days, the old test solution was collected in a clean beaker and returned to the test vessel along with the parent daphnid. On renewal days, the old test solution was collected from all test group replicates.
On these days the parent daphnids were returned to their test vessels with fresh test solution.
On day 21 of exposure, the surviving adults were removed from the test vessels and euthanized by immersion in 70% isopropanol.
Individuals were then isolated on a labeled glass slide. The body length of each adult daphnid was measured from the apex of the helmet to the base of the posterior spine (i.e. excluding the anal spine and protruding second antennae) with a binocular dissecting microscope (Leitz Diavert) and a calibrated eyepiece micrometer which was checked with a standardized slide.
Throughout the test, the appearance of the test solutions and dissolution behavior of the test
substance was observed and recorded daily. The chemical and physical parameters of the test medium (total hardness, acid capacity, pH, conductivity and total organic carbon) were determined after aeration and prior to use in the test and were within acceptable ranges. Dissolved oxygen, pH and temperature were measured in the old and in the freshly prepared test solution in replicate 1 of each test group for one interval per week.
In addition, temperature was measured continuously during the whole exposure period in a separate vessel filled with water proximal to the test vessels. Hardness was measured in the freshly prepared test solution in an additional replicate of each concentration for one interval per week and in the old test solution from the combined replicates of each test group for one interval per week.
The stability of the test substance as a solution in test media and under testing conditions was determined by concentration control analysis. The test solutions were visibly homogeneous.
Acclimatization:
The Daphnia are cultured under the identical conditions as the test including test media (Elendt M4), water quality, temperature (20 ±1°C), and diet.
Age at start of exposure: <24 h
Aeration: none
Test solution renewal: Semi-static, renewal 3 times per week
Diet: During the test daphnids were fed daily a diet of live green algae Desmodesmus subspicatus,
cultured in a synthetic medium. The algae were separated from their culture medium by
centrifugation, resuspended in daphnid's medium (M4) corresponding to concentrations of 1460 mg and 1513 mg TOC*/L (respectively) in the algal concentrates used.
The daphnids were fed a defined volume (≤137 μL) of the concentrate to reach the amount of food defined in the table below. The algae were stored in a refrigerator (dark, about 4-8°C) for maximum 21 days.
By adding the algal concentrate, the test solution was slightly diluted. During a 72-h test interval with 3 feedings, 0.137 mL each, a total of 0.411 mL are added to 50 mL test volume resulting in a maximal dilution of 0.8 %.
Feeding schedule:
Day of Test Amount of food per parent animal and day [mg TOC] *
0-3 0.10
4-5 0.12
6-7 0.14
8-test end 0.20
*amount of feed algae determined as total organic carbon.
Light intensity / Photo period:
801.8-837.4 lux at a wavelength of 400–750 nm. The light intensity did not exceed 1000–1500 lux for cool white light (equivalent to 15–20 μE•m-2•s-1 according to the test guideline.
16 hours light : 8 hours darkness
Test vessels : Numbered glass beakers (nominal volume 100 mL), covered with glass Petri plates to slow evaporation.
Test volume: 50 mL
Biological loading: 1 animal/test vessel (0.02 animals/mL)
Test groups :
0 (control), 1.56, 3.13, 6.25, 12.5, 25 mg/L as nominal concentrations based on test substance mass without correction for purity.
Replicates/test group:
10 replicates (1 parent animal each)
1 additional abiotic replicate (no Daphnia or food algae) per test group is used to help assess test substance stability over the renewal period.
Reason for the selection of the test concentrations:
According to the test guidelines, at least 5 concentrations in a geometric series with a separation factor ≤3.2 (OECD) should be used.
In a preliminary test (experimental conduct in accordance with GLP but without a GLP status), after 14 days exposure to the test substance, no significant effect on reproduction was observed at 12 mg/L.
Each test group initially consisted of 10 replicates containing one juvenile Daphnia (<24 h old) each.
One additional abiotic replicate (no Daphnia or food algae) per test group was used to help assess test substance stability over the renewal period.
Test group Nominal Concentration as test substance(a)
[mg/L]
0 (Control) 0
1 1.56
2 3.13
3 6.25
4 12.5
5 25
(a): Nominal concentrations based on test substance mass without correction for purity
Test substance preparation:
The stock test solutions (25 mg/L) were prepared by dissolving 50 mg of test substance in 2-L
of test medium and stirring for approximately 10 min at ambient temperature. The lower test
concentrations were prepared by dilution of this stock solution. Before the dilution was performed, the stock solution was checked for complete dissolution of the test substance.
Data evaluation:
The following parameters and statistical procedures (hypothesis tests) were used to evaluate significant differences to the control:
- Reproduction as number of living young (Wilcoxon’s test, one sided with Bonferroni Holm
adjustment, one sided analysis)
The following additional endpoints undergo statistical evaluation but are not used to establish LOEC and NOEC:
- % immobile young (Wilcoxon’s test, with Bonferroni Holm adjustment, one sided analysis)
- % aborted eggs (Wilcoxon’s test, one sided with Bonferroni Holm adjustment, one sided analysis) - Time to first brood (Wilcoxon’s test, with Bonferroni Holm adjustment, one sided analysis)
- Growth as length in mm (Dunnett´s test, one sided analysis)
The NOEC and LOEC are based on the primary population relevant parameters identified in the OECD test guideline, reproduction and mortality. For the evaluation of reproduction as number of living young two analyses were done. The first analysis only uses the data of surviving parents. The second analysis includes the data from all animals which do not die accidentally or inadvertently during the test.
This endpoint is called number of living young per introduced parent.
The percent reduction of number of living young, the percent reduction of length and percent
increase in time to first brood were calculated in relation to the control mean. Percentages
lower than 0 were set to 0. The percent immobile young in each test group was calculated in
relation to the sum of the number of immobile and number of living young. The percent aborted
eggs in each test group was calculated in relation to the sum of number of aborted eggs,
number of immobile and number of living young. The endpoints length, time to first brood,
immobile young and aborted eggs were evaluated only for organisms that survived to the end
of the test. - Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 25
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Purity 90.4%
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 25
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Purity 90.4%
- Details on results:
- Dissolution behavior:
All test solutions were visibly colorless and clear throughout each renewal period.
No undissolved test substance or other unusual manifestations were observed.
Analytical Results:
Recovery effficiency: 87-106%
All measured concentrations deviated less than 20% from the nominal concentration. Thus, the test substance was stable in solution under test conditions and nominal concentrations are an accurate representation of exposure levels maintained throughout the test period. Following
recommendations in OECD 211 the results should be evaluated based on the nominal
concentrations.
Additionally, samples were analyzed from the abiotic replicate of each test concentration and the control at the end of the renewal interval. All abiotic replicates were within ±20% of the nominal concentrations (see analytical report in the appendix). These samples were analyzed to assist in the interpretation of results in the event that food algae or Daphnia over the exposure period interfered with the test substance analyses. Since the biotic test replicate samples were all within ±20% of the nominal, the additional analyses of abiotic replicates have no influence on the interpretation of this study and are only presented in the appendix with the analytical report.
Biological results:
The OECD 211 test guideline is primarily designed to assess effects on Daphnia reproductive output (living young)nas the primary population relevant endpoint. Measuring and reporting other biological parameters such as growth, %immobile young, time to first brood and %aborted eggs is optional. These biological parameters in addition to reproduction may assist in the overall interpretation of the study results; however, they alone are not indicative of a population relevant effect. A statistically significant difference in these additional parameters without an effect on reproduction is not considered in establishing the NOEC.
No significant mortality or any other additional significant adverse effects or abnormal behavior
were observed in any of the test treatments.
The following overall effect concentrations (mg/L) are based on reproduction (living offspring per surviving adult) as the primary population relevant response and were obtained based on nominal concentrations:
LOEC = > 25 mg/L
NOEC = ≥ 25 mg/L
Since the measured concentrations of the test substance in the test solutions were within +20% of the nominal concentrations, the effect concentration can be expressed relative to the nominal concentration.
The data were not sufficient to calculate ECx values for reproduction or mortality.
No significant mortality or any other additional significant adverse effects or abnormal behavior
were observed in any of the test treatments.
The toxicity results presented here are consistent with the results from preliminary tests.
The results in this study are consistent with all validity criteria and the test is valid according to the guidelines of this study.
No deviations from test guidelines or other incidents occurred during the course of the reported test which may have influenced the results.
Validity criteria:
This test was fully compliant with all the following validity criteria required by the corresponding test guidelines and is considered valid.
• In the control the mortality of parent animals up to the end of the test should be ≤20%. The mortality in the control was 0%
• In the control the mean number of living young per parent animal that survived the test should be ≥60.
In the control the mean number of living young per parent animal was 139.7. - Results with reference substance (positive control):
- In order to verify that the Daphnia magna culture is responding normally to toxic stress, acute toxicity tests with a reference substance are conducted monthly. Reference substance tests are conducted generally according to the OECD 202 guideline and in accordance with GLP, but without a GLP status.
The EC50(48 h) of the reference substance sodium chloride (NaCl) was 3.88 g/L (experiment date: 22 Sep 2021, project number: 50E0789/12E064).
This result is within the range of 3.88–7.22 g/L, which represents ±2 standard deviations from the published EC50(48 h) of 5.55 g/L and indicates that the culture of Daphnia magna used in this study is responding normally to toxic stress. - Reported statistics and error estimates:
- Tables and/or figures of measured parameters were produced using relevant computing software. All reported data values are rounded to the appropriate significant figures based on the precision of the analytical method and/or consistent with the requirements in the pertinent test guideline(s). T
Calculations to determine the LOEC and NOEC were carried out using SAS System computer software.
The following parameters and statistical procedures (hypothesis tests) were
used to evaluate significant differences to the control:
- Reproduction as number of living young (Wilcoxon’s test, one sided with Bonferroni Holm adjustment, one sided analysis)
- Parent mortality as immobilized organisms (Fishers exact test, one sided analysis)
The following additional endpoints undergo statistical evaluation but are not used to establish LOEC and NOEC:
- % immobile young (Wilcoxon’s test, with Bonferroni Holm adjustment, one sided analysis)
- % aborted eggs (Wilcoxon’s test, one sided with Bonferroni Holm adjustment, one sided analysis)
- Time to first brood (Wilcoxon’s test, with Bonferroni Holm adjustment, one sided analysis)
- Growth as length in mm (Dunnett´s test, one sided analysis)
The NOEC and LOEC are based on the primary population relevant parameters identified in the OECD test guideline, reproduction and mortality. For the evaluation of reproduction
as number of living young two analyses were done. The first analysis only uses the data of
surviving parents. The second analysis includes the data from all animals which do not die
accidentally or inadvertently during the test. This endpoint is called number of living young per
introduced parent.
The percent reduction of number of living young, the percent reduction of length and percent
increase in time to first brood were calculated in relation to the control mean. Percentages
lower than 0 were set to 0. - Validity criteria fulfilled:
- yes
- Conclusions:
- The effects on reproduction were evaluated based on the reproduction (living offspring per surviving adult) as the primary population relevant response for the most sensitive endpoint (reproduction) and were obtained based on nominal concentrations:
LOEC > 25 mg/L
NOEC >= 25 mg/L.
Since the analytically determined concentrations of the test substance in the test solutions were within the range of ±20% of the nominal concentrations, the effect concentration can be expressed relative to the nominal concentration.
No significant mortality, reduced reproduction, or any other additional significant adverse effects or abnormal behavior were observed in any of the test treatments. The data were not sufficient to calculate ECx values for reproduction or mortality. - Executive summary:
- A Daphnia magna reproduction test (semi-static, 21 d) of the test item EDTA-ZnK2 (batch number: FC-C 11530) was conducted according to OECD 211 (2012) from 2021-Jul-07 to 2022-Mar-03, with the definitive exposure phase from 2021-Sep-22 to 2021-Oct-13 at BASF SE.
Test species was Daphnia magna STRAUS. Ten daphnids, held individually, were used per nominal test item concentration and control. At the start of the exposure the daphnids were less than 24 hours old. The study was carried out under semi-static conditions with medium exchange three times per week. Aim of the test was to assess the effects on the reproduction capacity and other test item-related effects or parameters such as the intrinsic rate of the natural increase, the number of broods with living juveniles, first appearance of living juveniles, occurrence of aborted eggs and stillborn juveniles, adult mortality, body length and dry weight of the parental daphnids.
The test item EDTA-ZnK2 is a white solid. The aqueous media were prepared dissolving 50 mg of test substance in 2-L of test medium and stirring for approximately 10 min at ambient temperature. The lower test concentrations were prepared by dilution of this stock solution. Before the dilution was performed, the stock solution was checked for complete dissolution of the test substance. Five test concentrations were prepared and tested nominal concentration rates 25 – 12.5 – 6.25 – 3.13 - 1.56 mg//L (separation factor 2.0).
The stock solutions were checked visually to ensure that the test substance was completely dissolved.
The test item concentrations of EDTA-ZnK2 were analytically verified via LC-MS three times during the test (once within a period of 7 days) in the fresh media at the start of an exposure-renewal interval (0 hours; on test days 0, 9 and 19) as well as in the old media at the end of an exposure-renewal interval (24 hours; on test days 2, 12 and 21) in all test item concentrations and in the control.
The environmental conditions were within the acceptable limits. The validity criteria of the test guideline were met.The OECD 211 test guideline is primarily designed to assess effects on Daphnia reproductive output (living young) as the primary population relevant endpoint. Measuring and reporting other biological parameters such as growth, %immobile young, time to first brood and %aborted eggs is optional. These biological parameters in addition to reproduction may assist in the overall interpretation of the study results; however, they alone are not indicative of a population relevant effect. A statistically significant difference in these additional parameters without an effect on reproduction is not considered in establishing the NOEC. No significant mortality or any other additional significant adverse effects or abnormal behavior were observed in any of the test treatments. The following overall effect concentrations (mg/L) are based on reproduction (living offspring per surviving adult) as the primary population relevant response and were obtained based on nominal concentrations:
LOEC = > 25 mg/L
NOEC = ≥ 25 mg/L
Since the measured concentrations of the test substance in the test solutions were within +20% of the nominal concentrations, the effect concentration can be expressed relative to the nominal concentration.The purity of the test substance used in the test was 90.4%
LOEC = > 25 mg test substance/L or 22.6 mg a.i./L
NOEC = ≥ 25 mg test substance/L or 22.6 mg a.i./L- A Daphnia magna reproduction test (semi-static, 21 d) of the test item EDTA-ZnK2 (batch number: FC-C 11530) was conducted according to OECD 211 (2012) from 2021-Jul-07 to 2022-Mar-03, with the definitive exposure phase from 2021-Sep-22 to 2021-Oct-13 at BASF SE.
Reference
Measured values for the oxygen content [mg/L], measured in replicate 1 per test conc.:
Day | 0 (a) | 2 (b) | 9 (a) | 12 (b) | 19 (a) | 21 (b) |
Nominal concentration [mg/L] | ||||||
0 | 9.0 | 9.1 | 8.8 | 9.3 | 9.0 | 8.8 |
1.56 | 8.9 | 9.0 | 8.8 | 9.3 | 8.9 | 8.8 |
3.13 | 9.3 | 9.0 | 8.8 | 9.1 | 8.9 | 8.7 |
6.25 | 9.3 | 9.1 | 8.7 | 9.3 | 8.9 | 8.6 |
12.5 | 9.2 | 9.1 | 8.8 | 9.3 | 8.9 | 8.8 |
25 | 8.9 | 9.1 | 8.8 | 9.3 | 8.9 | 8.8 |
a: in the freshly prepared test solutions
b: before renewal in the old test solutions
pH-values, measured in replicate 1 per test concentration:
Day | 0 (a) | 2 (b) | 9 (a) | 12 (b) | 19 (a) | 21 (b) |
Nominal concentration [mg/L] | ||||||
0 | 7.8 | 8.0 | 7.7 | 8.0 | 7.8 | 7.9 |
1.56 | 7.8 | 8.1 | 7.7 | 7.9 | 7.8 | 7.8 |
3.13 | 7.8 | 8.0 | 7.8 | 7.8 | 7.8 | 7.8 |
6.25 | 7.8 | 8.0 | 7.8 | 7.8 | 7.8 | 7.7 |
12.5 | 7.8 | 8.1 | 7.8 | 7.8 | 7.8 | 7.7 |
25 | 7.8 | 8.1 | 7.8 | 7.8 | 7.8 | 7.7 |
a: in the freshly prepared test solutions
b: before renewal in the old test solutions
Instantaneous temperature (ºC), measured in replicate 1 per test concentration:
Day | 0 (a) | 2 (b) | 9 (a) | 12 (b) | 19 (a) | 21 (b) |
Nominal concentration [mg/L] | ||||||
0 | 19.8 | 20.2 | 19.9 | 19.9 | 19.9 | 19.6 |
1.56 | 19.8 | 20.2 | 19.9 | 19.8 | 19.8 | 19.6 |
3.13 | 19.8 | 20.2 | 19.9 | 19.9 | 19.9 | 19.7 |
6.25 | 19.8 | 20.1 | 19.9 | 20.0 | 19.9 | 19.6 |
12.5 | 19.8 | 20.1 | 19.9 | 19.9 | 19.8 | 19.7 |
25 | 19.8 | 20.0 | 19.9 | 19.9 | 19.8 | 19.6 |
a: in the freshly prepared test solutions
b: before renewal in the old test solutions
Additionally, temperature was measured continuously during the exposure period in a separate
water filled vessel proximal to the test vessels. The temperature ranged from 19.4-20.3°C.
Measured values for the total hardness [mmol/L]:
Day | 0 (a) | 2 (b) | 9 (a) | 12 (b) | 19 (a) | 21 (b) |
Nominal concentration [mg/L] | ||||||
0 | 2.34 | 2.37 | 2.39 | 2.39 | 2.35 | 2.26 |
1.56 | 2.36 | 2.37 | 2.40 | 2.38 | 2.34 | 2.30 |
3.13 | 2.36 | 2.37 | 2.39 | 2.38 | 2.29 | 2.29 |
6.25 | 2.34 | 2.37 | 2.37 | 2.39 | 2.32 | 2.27 |
12.5 | 2.34 | 2.37 | 2.37 | 2.39 | 2.27 | 2.28 |
25 | 2.36 | 2.37 | 2.37 | 2.39 | 2.28 | 2.31 |
a: in the freshly prepared test solutions
b: before renewal in the old test solutions
Total number of living young per surviving parent animal after the 21 day exposure period. Base for the data are the values standardized on the number of surviving parent animals of each of the ten replicates.
nominal concentration | cv | |||||||||||||
mg/L | Rep. 1 | Rep. 2 | Rep. 3 | Rep. 4 | Rep. 5 | Rep. 6 | Rep. 7 | Rep. 8 | Rep. 9 | Rep. 10 | sum | mean | sd | [%] |
0 | 158 | 125 | 165 | 151 | 165 | 121 | 139 | 126 | 134 | 113 | 1397 | 139.7 | 19.0 | 13.6 |
1.56 | 157 | 165 | 142 | 155 | 92 | 172 | 136 | 143 | 126 | 136 | 1424 | 142.4 | 22.7 | 16.0 |
3.13 | 175 | 176 | 147 | 166 | 143 | 154 | 99 | 164 | 148 | 122 | 1494 | 149.4 | 24.1 | 16.1 |
6.25 | 157 | 145 | 131 | 183 | 159 | 154 | 154 | 146 | 149 | 148 | 1526 | 152.6 | 13.3 | 8.7 |
12.5 | 161 | 163 | 161 | 123 | 158 | 156 | 151 | 146 | 154 | 139 | 1512 | 151.2 | 12.4 | 8.2 |
25 | 153 | 128 | 125 | 144 | 154 | 136 | 115 | 111 | 85 | 155 | 1306 | 130.6 | 22.6 | 17.3 |
cv = coefficient of variance |
sd = standard deviation |
Total number of immobile young per surviving parent animal after the 21 day exposure period. Base for the data are the values standardized on the number of surviving parent animals of each of the ten replicates.
nominal concentration | cv | |||||||||||||
mg/L | Rep. 1 | Rep. 2 | Rep. 3 | Rep. 4 | Rep. 5 | Rep. 6 | Rep. 7 | Rep. 8 | Rep. 9 | Rep. 10 | sum | mean | sd | [%] |
0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0 | 0.0 | 0.0 | 0.0 |
1.56 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 3.0 | 0.3 | 0.7 | 225.0 |
3.13 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0 | 0.0 | 0.0 | 0.0 |
6.25 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0 | 0.0 | 0.0 | 0.0 |
12.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0 | 0.0 | 0.0 | 0.0 |
25 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0 | 0.0 | 0.0 | 0.0 |
cv = coefficient of variance |
sd = standard deviation |
Total number of aborted subitane eggs per surviving parent animal after the 21 day exposure period. Base for the data are the values standardized on the number of surviving parent animals of each of the ten replicates.
nominal concentration | cv | |||||||||||||
mg/L | Rep.1 | Rep.2 | Rep.3 | Rep.4 | Rep.5 | Rep.6 | Rep.7 | Rep.8 | Rep.9 | Rep.10 | sum | mean | sd | [%] |
0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 0.3 | 0.5 | 161.0 |
1.56 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 3 | 0.3 | 0.5 | 161.0 |
3.13 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0 | 0.0 | 0.0 |
6.25 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0.1 | 0.3 | 316.2 |
12.5 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 5 | 0.5 | 0.5 | 105.4 |
25 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 0.2 | 0.6 | 316.2 |
cv = coefficient of variance |
sd = standard deviation |
Total number of surviving parent animals per test day at each concentration.
nominal concentration | day of the test | % Mortality (21d) | |||||||||||||||||||||
mg/L | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | |
0 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 0% |
1.56 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 0% |
3.13 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 0% |
6.25 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 0% |
12.5 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 0% |
25 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 0% |
Length of the parent animals
length [mm] | |||||||||||||
nominal concentration [mg/L] | Rep. 1 | Rep. 2 | Rep. 3 | Rep. 4 | Rep. 5 | Rep. 6 | Rep. 7 | Rep. 8 | Rep. 9 | Rep. 10 | mean | sd | cv [%] |
0 | 4.048 | 4.476 | 4.429 | 4.667 | 4.333 | 4.524 | 4.476 | 4.429 | 4.429 | 4.286 | 4.410 | 0.164 | 3.71 |
1.56 | 4.619 | 4.571 | 4.524 | 4.524 | 4.571 | 4.524 | 4.286 | 4.571 | 4.190 | 4.476 | 4.486 | 0.138 | 3.08 |
3.13 | 4.619 | 4.429 | 4.476 | 4.286 | 4.381 | 4.524 | 4.143 | 4.619 | 4.238 | 4.333 | 4.405 | 0.159 | 3.61 |
6.25 | 4.381 | 4.429 | 4.381 | 4.619 | 4.333 | 4.476 | 4.571 | 4.333 | 4.524 | 4.524 | 4.457 | 0.101 | 2.26 |
12.5 | 4.571 | 4.429 | 4.429 | 4.429 | 4.381 | 4.524 | 4.476 | 3.810 | 4.286 | 4.476 | 4.381 | 0.215 | 4.91 |
25 | 4.190 | 4.524 | 4.429 | 4.524 | 4.476 | 4.429 | 4.286 | 4.286 | 4.476 | 4.381 | 4.400 | 0.113 | 2.56 |
cv = coefficient of variance |
sd = standard deviation |
Table and summarized evaluation of the daily single values of the amount of young (total, alive and dead), |
of the aborted subitane eggs and the living parent animals. |
nominal concentration of the test substance: 25 mg/L |
day | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | sum | ||
vessel No. | |||||||||||||||||||||||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 0 | 27 | 0 | 0 | 32 | 0 | 0 | 0 | 41 | 0 | 0 | 43 | 153 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
1 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 0 | 27 | 0 | 0 | 32 | 0 | 0 | 0 | 41 | 0 | 0 | 43 | 153 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 18 | 0 | 0 | 31 | 0 | 0 | 39 | 1 | 0 | 39 | 0 | 0 | 128 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
2 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 18 | 0 | 0 | 31 | 0 | 0 | 39 | 1 | 0 | 39 | 0 | 0 | 128 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 16 | 0 | 0 | 35 | 0 | 0 | 0 | 34 | 0 | 0 | 40 | 0 | 125 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
3 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 16 | 0 | 0 | 35 | 0 | 0 | 0 | 34 | 0 | 0 | 40 | 0 | 125 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 24 | 0 | 0 | 31 | 0 | 0 | 36 | 0 | 0 | 39 | 144 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
4 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 24 | 0 | 0 | 31 | 0 | 0 | 36 | 0 | 0 | 39 | 144 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 5 | 0 | 27 | 0 | 0 | 37 | 0 | 0 | 32 | 0 | 0 | 40 | 154 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
5 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 5 | 0 | 27 | 0 | 0 | 37 | 0 | 0 | 32 | 0 | 0 | 40 | 154 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 0 | 0 | 19 | 0 | 0 | 32 | 0 | 0 | 36 | 0 | 0 | 38 | 136 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
6 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 0 | 0 | 19 | 0 | 0 | 32 | 0 | 0 | 36 | 0 | 0 | 38 | 136 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 16 | 0 | 0 | 26 | 0 | 0 | 36 | 0 | 0 | 0 | 37 | 115 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
7 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 16 | 0 | 0 | 26 | 0 | 0 | 36 | 0 | 0 | 0 | 37 | 115 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 0 | 0 | 24 | 0 | 0 | 36 | 0 | 0 | 36 | 0 | 0 | 0 | 111 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
8 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 0 | 0 | 24 | 0 | 0 | 36 | 0 | 0 | 36 | 0 | 0 | 0 | 111 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 30 | 0 | 0 | 41 | 0 | 0 | 0 | 0 | 0 | 85 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
9 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 30 | 0 | 0 | 41 | 0 | 0 | 0 | 0 | 0 | 85 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 26 | 0 | 0 | 40 | 0 | 0 | 37 | 0 | 0 | 38 | 155 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
10 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 26 | 0 | 0 | 40 | 0 | 0 | 37 | 0 | 0 | 38 | 155 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
| Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 77 | 53 | 43 | 120 | 96 | 58 | 176 | 80 | 71 | 218 | 39 | 40 | 235 | 1306 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 77 | 53 | 43 | 120 | 96 | 58 | 176 | 80 | 71 | 218 | 39 | 40 | 235 | 1306 | |||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | |||
Palive | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | ||||
P = Parent animal, S = Subitane egg, Y = Young |
Table and summarized evaluation of the daily single values of the amount of young (total, alive and dead), |
of the aborted subitane eggs and the living parent animals. |
nominal concentration of the test substance: 12.5 mg/L |
day | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | sum | ||
vessel No. | |||||||||||||||||||||||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 0 | 28 | 0 | 0 | 0 | 37 | 0 | 0 | 44 | 0 | 0 | 39 | 161 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
1 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 0 | 28 | 0 | 0 | 0 | 37 | 0 | 0 | 44 | 0 | 0 | 39 | 161 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 12 | 0 | 29 | 0 | 0 | 38 | 0 | 0 | 0 | 41 | 0 | 0 | 43 | 163 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
2 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 12 | 0 | 29 | 0 | 0 | 38 | 0 | 0 | 0 | 41 | 0 | 0 | 43 | 163 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 0 | 0 | 28 | 0 | 0 | 38 | 0 | 0 | 39 | 0 | 0 | 43 | 161 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
3 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 0 | 0 | 28 | 0 | 0 | 38 | 0 | 0 | 39 | 0 | 0 | 43 | 161 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 18 | 0 | 0 | 32 | 0 | 0 | 49 | 0 | 0 | 0 | 24 | 0 | 123 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
4 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 18 | 0 | 0 | 32 | 0 | 0 | 49 | 0 | 0 | 0 | 24 | 0 | 123 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 31 | 0 | 0 | 36 | 0 | 0 | 0 | 41 | 0 | 0 | 36 | 158 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
5 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 31 | 0 | 0 | 36 | 0 | 0 | 0 | 41 | 0 | 0 | 36 | 158 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 16 | 0 | 0 | 33 | 0 | 0 | 39 | 0 | 0 | 36 | 0 | 0 | 32 | 156 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
6 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 16 | 0 | 0 | 33 | 0 | 0 | 39 | 0 | 0 | 36 | 0 | 0 | 32 | 156 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 0 | 0 | 25 | 0 | 0 | 38 | 0 | 0 | 40 | 0 | 0 | 38 | 151 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
7 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 0 | 0 | 25 | 0 | 0 | 38 | 0 | 0 | 40 | 0 | 0 | 38 | 151 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 0 | 0 | 25 | 0 | 0 | 38 | 0 | 0 | 38 | 0 | 0 | 32 | 146 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
8 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 0 | 0 | 25 | 0 | 0 | 38 | 0 | 0 | 38 | 0 | 0 | 32 | 146 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 0 | 0 | 30 | 0 | 0 | 36 | 0 | 0 | 37 | 0 | 0 | 43 | 154 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
9 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 0 | 0 | 30 | 0 | 0 | 36 | 0 | 0 | 37 | 0 | 0 | 43 | 154 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 12 | 0 | 0 | 12 | 0 | 0 | 38 | 0 | 0 | 38 | 0 | 0 | 39 | 139 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
10 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 12 | 0 | 0 | 12 | 0 | 0 | 38 | 0 | 0 | 38 | 0 | 0 | 39 | 139 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
| Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 97 | 18 | 88 | 153 | 32 | 74 | 264 | 49 | 0 | 354 | 0 | 24 | 345 | 1512 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 97 | 18 | 88 | 153 | 32 | 74 | 264 | 49 | 0 | 354 | 0 | 24 | 345 | 1512 | |||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 5 | |||
Palive | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | ||||
P = Parent animal, S = Subitane egg, Y = Young |
Table and summarized evaluation of the daily single values of the amount of young (total, alive and dead), |
of the aborted subitane eggs and the living parent animals. |
nominal concentration of the test substance: 6.25 mg/L |
day | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | sum | ||
vessel No. | |||||||||||||||||||||||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 0 | 0 | 29 | 0 | 0 | 38 | 0 | 0 | 25 | 15 | 0 | 0 | 39 | 157 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
1 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 0 | 0 | 29 | 0 | 0 | 38 | 0 | 0 | 25 | 15 | 0 | 0 | 39 | 157 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 0 | 0 | 27 | 0 | 0 | 32 | 0 | 0 | 34 | 0 | 0 | 37 | 145 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
2 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 0 | 0 | 27 | 0 | 0 | 32 | 0 | 0 | 34 | 0 | 0 | 37 | 145 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 17 | 0 | 0 | 30 | 0 | 0 | 41 | 0 | 0 | 43 | 0 | 0 | 131 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
3 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 17 | 0 | 0 | 30 | 0 | 0 | 41 | 0 | 0 | 43 | 0 | 0 | 131 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 2 | 0 | 35 | 0 | 0 | 46 | 0 | 0 | 0 | 43 | 0 | 0 | 42 | 183 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
4 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 2 | 0 | 35 | 0 | 0 | 46 | 0 | 0 | 0 | 43 | 0 | 0 | 42 | 183 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 24 | 0 | 0 | 41 | 0 | 0 | 42 | 0 | 0 | 38 | 159 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
5 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 24 | 0 | 0 | 41 | 0 | 0 | 42 | 0 | 0 | 38 | 159 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 12 | 0 | 25 | 0 | 0 | 29 | 0 | 0 | 0 | 45 | 0 | 0 | 43 | 154 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
6 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 12 | 0 | 25 | 0 | 0 | 29 | 0 | 0 | 0 | 45 | 0 | 0 | 43 | 154 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 0 | 24 | 0 | 0 | 13 | 25 | 0 | 0 | 39 | 0 | 0 | 38 | 154 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
7 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 0 | 24 | 0 | 0 | 13 | 25 | 0 | 0 | 39 | 0 | 0 | 38 | 154 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 0 | 0 | 21 | 0 | 0 | 37 | 0 | 0 | 41 | 0 | 0 | 36 | 146 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
8 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 0 | 0 | 21 | 0 | 0 | 37 | 0 | 0 | 41 | 0 | 0 | 36 | 146 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 12 | 0 | 0 | 26 | 0 | 0 | 35 | 0 | 0 | 36 | 0 | 0 | 40 | 149 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
9 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 12 | 0 | 0 | 26 | 0 | 0 | 35 | 0 | 0 | 36 | 0 | 0 | 40 | 149 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 12 | 0 | 30 | 0 | 0 | 37 | 0 | 0 | 0 | 37 | 0 | 0 | 32 | 148 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
10 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 12 | 0 | 30 | 0 | 0 | 37 | 0 | 0 | 0 | 37 | 0 | 0 | 32 | 148 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
| Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 26 | 93 | 17 | 143 | 98 | 30 | 163 | 170 | 41 | 25 | 332 | 43 | 0 | 345 | 1526 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 26 | 93 | 17 | 143 | 98 | 30 | 163 | 170 | 41 | 25 | 332 | 43 | 0 | 345 | 1526 | |||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | |||
Palive | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | ||||
P = Parent animal, S = Subitane egg, Y = Young |
Table and summarized evaluation of the daily single values of the amount of young (total, alive and dead), |
of the aborted subitane eggs and the living parent animals. |
nominal concentration of the test substance: 3.13 mg/L |
day | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | sum | ||
vessel No. | |||||||||||||||||||||||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 32 | 0 | 0 | 44 | 0 | 0 | 39 | 0 | 0 | 0 | 46 | 175 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
1 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 32 | 0 | 0 | 44 | 0 | 0 | 39 | 0 | 0 | 0 | 46 | 175 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 16 | 10 | 0 | 22 | 0 | 0 | 46 | 0 | 0 | 41 | 0 | 0 | 0 | 41 | 176 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
2 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 16 | 10 | 0 | 22 | 0 | 0 | 46 | 0 | 0 | 41 | 0 | 0 | 0 | 41 | 176 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 0 | 0 | 28 | 0 | 0 | 38 | 0 | 0 | 34 | 0 | 0 | 34 | 147 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
3 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 0 | 0 | 28 | 0 | 0 | 38 | 0 | 0 | 34 | 0 | 0 | 34 | 147 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 12 | 0 | 31 | 0 | 0 | 42 | 0 | 0 | 0 | 40 | 0 | 0 | 41 | 166 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
4 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 12 | 0 | 31 | 0 | 0 | 42 | 0 | 0 | 0 | 40 | 0 | 0 | 41 | 166 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 0 | 0 | 29 | 0 | 0 | 32 | 0 | 0 | 37 | 0 | 0 | 38 | 143 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
5 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 0 | 0 | 29 | 0 | 0 | 32 | 0 | 0 | 37 | 0 | 0 | 38 | 143 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 0 | 29 | 0 | 0 | 33 | 0 | 0 | 0 | 40 | 0 | 0 | 37 | 154 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
6 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 0 | 29 | 0 | 0 | 33 | 0 | 0 | 0 | 40 | 0 | 0 | 37 | 154 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 0 | 0 | 22 | 0 | 0 | 31 | 1 | 0 | 34 | 0 | 0 | 99 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
7 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 0 | 0 | 22 | 0 | 0 | 31 | 1 | 0 | 34 | 0 | 0 | 99 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 0 | 29 | 0 | 0 | 39 | 0 | 0 | 0 | 42 | 0 | 0 | 43 | 164 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
8 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 0 | 29 | 0 | 0 | 39 | 0 | 0 | 0 | 42 | 0 | 0 | 43 | 164 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 0 | 0 | 22 | 0 | 0 | 34 | 0 | 0 | 36 | 0 | 0 | 43 | 148 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
9 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 0 | 0 | 22 | 0 | 0 | 34 | 0 | 0 | 36 | 0 | 0 | 43 | 148 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 17 | 0 | 0 | 23 | 0 | 0 | 32 | 0 | 0 | 38 | 0 | 0 | 12 | 122 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
10 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 17 | 0 | 0 | 23 | 0 | 0 | 32 | 0 | 0 | 38 | 0 | 0 | 12 | 122 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
| Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 30 | 98 | 11 | 143 | 102 | 22 | 204 | 136 | 31 | 81 | 267 | 34 | 0 | 335 | 1494 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 30 | 98 | 11 | 143 | 102 | 22 | 204 | 136 | 31 | 81 | 267 | 34 | 0 | 335 | 1494 | |||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | ||||
P = Parent animal, S = Subitane egg, Y = Young |
Table and summarized evaluation of the daily single values of the amount of young (total, alive and dead), |
of the aborted subitane eggs and the living parent animals. |
nominal concentration of the test substance: 1.56 mg/L |
day | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | sum | ||
vessel No. | |||||||||||||||||||||||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 30 | 0 | 0 | 34 | 0 | 0 | 39 | 0 | 0 | 40 | 0 | 157 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
1 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 30 | 0 | 0 | 34 | 0 | 0 | 39 | 0 | 0 | 40 | 0 | 157 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 0 | 0 | 31 | 0 | 0 | 39 | 0 | 0 | 40 | 0 | 0 | 0 | 40 | 165 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
2 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 0 | 0 | 31 | 0 | 0 | 39 | 0 | 0 | 40 | 0 | 0 | 0 | 40 | 165 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 0 | 0 | 28 | 0 | 0 | 34 | 0 | 0 | 36 | 0 | 0 | 29 | 142 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
3 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 0 | 0 | 28 | 0 | 0 | 34 | 0 | 0 | 36 | 0 | 0 | 29 | 142 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 23 | 0 | 0 | 42 | 0 | 0 | 35 | 0 | 0 | 0 | 41 | 155 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
4 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 23 | 0 | 0 | 42 | 0 | 0 | 35 | 0 | 0 | 0 | 41 | 155 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 8 | 0 | 0 | 20 | 0 | 0 | 16 | 18 | 0 | 0 | 16 | 92 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
5 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 0 | 0 | 8 | 0 | 0 | 20 | 0 | 0 | 16 | 18 | 0 | 0 | 16 | 92 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 17 | 0 | 0 | 35 | 0 | 0 | 42 | 0 | 0 | 40 | 0 | 0 | 0 | 40 | 174 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | |||
6 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 0 | 0 | 35 | 0 | 0 | 42 | 0 | 0 | 40 | 0 | 0 | 0 | 40 | 172 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 0 | 0 | 25 | 0 | 0 | 33 | 0 | 0 | 37 | 0 | 0 | 32 | 137 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | |||
7 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 0 | 0 | 25 | 0 | 0 | 32 | 0 | 0 | 37 | 0 | 0 | 32 | 136 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 0 | 0 | 22 | 0 | 0 | 32 | 0 | 0 | 37 | 0 | 0 | 41 | 143 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
8 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 0 | 0 | 22 | 0 | 0 | 32 | 0 | 0 | 37 | 0 | 0 | 41 | 143 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 18 | 0 | 0 | 29 | 0 | 0 | 38 | 0 | 0 | 41 | 0 | 0 | 126 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
9 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 18 | 0 | 0 | 29 | 0 | 0 | 38 | 0 | 0 | 41 | 0 | 0 | 126 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 19 | 0 | 0 | 28 | 0 | 0 | 43 | 1 | 0 | 0 | 45 | 0 | 136 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
10 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 19 | 0 | 0 | 28 | 0 | 0 | 43 | 1 | 0 | 0 | 45 | 0 | 136 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
| Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 74 | 36 | 37 | 127 | 75 | 57 | 177 | 99 | 81 | 171 | 128 | 41 | 85 | 239 | 1427 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | |||
Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 72 | 36 | 37 | 127 | 75 | 57 | 177 | 98 | 81 | 171 | 128 | 41 | 85 | 239 | 1424 | |||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 3 | |||
Palive | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | ||||
P = Parent animal, S = Subitane egg, Y = Young |
Table and summarized evaluation of the daily single values of the amount of young (total, alive and dead), |
of the aborted subitane eggs and the living parent animals. |
nominal concentration of the test substance: 0 mg/L |
day | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | sum | ||
vessel No. | |||||||||||||||||||||||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 0 | 0 | 26 | 0 | 0 | 38 | 0 | 0 | 43 | 0 | 0 | 41 | 0 | 158 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
1 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 0 | 0 | 26 | 0 | 0 | 38 | 0 | 0 | 43 | 0 | 0 | 41 | 0 | 158 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 16 | 0 | 0 | 30 | 0 | 0 | 42 | 0 | 0 | 37 | 0 | 0 | 125 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
2 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 16 | 0 | 0 | 30 | 0 | 0 | 42 | 0 | 0 | 37 | 0 | 0 | 125 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 0 | 0 | 31 | 0 | 0 | 40 | 0 | 0 | 38 | 0 | 0 | 0 | 41 | 165 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
3 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 0 | 0 | 31 | 0 | 0 | 40 | 0 | 0 | 38 | 0 | 0 | 0 | 41 | 165 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 0 | 26 | 0 | 0 | 34 | 0 | 0 | 0 | 39 | 0 | 0 | 44 | 151 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
4 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 0 | 26 | 0 | 0 | 34 | 0 | 0 | 0 | 39 | 0 | 0 | 44 | 151 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 0 | 28 | 0 | 0 | 34 | 0 | 0 | 0 | 44 | 0 | 0 | 46 | 165 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
5 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 0 | 28 | 0 | 0 | 34 | 0 | 0 | 0 | 44 | 0 | 0 | 46 | 165 | |
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | ||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 17 | 0 | 0 | 27 | 0 | 0 | 35 | 0 | 0 | 42 | 0 | 0 | 121 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
6 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 17 | 0 | 0 | 27 | 0 | 0 | 35 | 0 | 0 | 42 | 0 | 0 | 121 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 17 | 0 | 0 | 33 | 0 | 0 | 43 | 0 | 0 | 46 | 0 | 0 | 139 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
7 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 17 | 0 | 0 | 33 | 0 | 0 | 43 | 0 | 0 | 46 | 0 | 0 | 139 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 16 | 0 | 0 | 32 | 0 | 0 | 40 | 1 | 0 | 0 | 37 | 0 | 126 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
8 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 16 | 0 | 0 | 32 | 0 | 0 | 40 | 1 | 0 | 0 | 37 | 0 | 126 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 0 | 0 | 27 | 0 | 0 | 23 | 0 | 0 | 38 | 0 | 0 | 38 | 134 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
9 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 0 | 0 | 27 | 0 | 0 | 23 | 0 | 0 | 38 | 0 | 0 | 38 | 134 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 0 | 0 | 31 | 0 | 0 | 38 | 0 | 0 | 33 | 0 | 113 | |||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
10 | Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 0 | 0 | 31 | 0 | 0 | 38 | 0 | 0 | 33 | 0 | 113 | ||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Palive | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
| Ytotal | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 25 | 29 | 66 | 122 | 27 | 122 | 177 | 23 | 160 | 120 | 121 | 125 | 111 | 169 | 1397 | ||
Ydead | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||
Yalive | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 25 | 29 | 66 | 122 | 27 | 122 | 177 | 23 | 160 | 120 | 121 | 125 | 111 | 169 | 1397 | |||
Saborted | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 3 | |||
Palive | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | ||||
P = Parent animal, S = Subitane egg, Y = Young |
Summarized analytically measured concentrations of EDTA-CuK2 in the test solutions:
Analytically Measured Concentration | ||||||
Nominal Concentration [mg/L] | Renewal period | Initial | 48h/72h old | Time-weighted Mean | ||
[mg/L] | [mg/L] | [mg/L]a | [%]b | [%]c | ||
0 (Control) | Day 0-20 | 0 | 0 | 0.0 | - | - |
Day 9-12 | 0 | 0 | ||||
Day 19-21 | 0 | 0 | ||||
1.56 | Day 0-2 | 1.63 | 1.55 | 1.51 | 98 | 97 |
Day 9-12 | 1.40 | 1.46 | ||||
Day 19-21 | 1.56 | 1.52 | ||||
3.13 | Day 0-2 | 3.26 | 3.12 | 3.06 | 100 | 98 |
Day 9-12 | 2.87 | 3.09 | ||||
Day 19-21 | 3.08 | 3.05 | ||||
6.3 | Day 0-2 | 6.48 | 6.12 | 6.08 | 100 | 97 |
Day 9-12 | 5.66 | 5.86 | ||||
Day 19-21 | 6.09 | 6.58 | ||||
12.5 | Day 0-2 | 12.8 | 12.4 | 12.3 | 101 | 99 |
Day 9-12 | 11.6 | 12.9 | ||||
Day 19-21 | 12.3 | 12.2 | ||||
25 | Day 0-2 | 25 | 24 | 24 | 99 | 97 |
Day 9-12 | 24 | 24 | ||||
Day 19-21 | 24 | 24 | ||||
a: based on 3 renewal period days. | ||||||
b: % of Mean Initial | ||||||
c: % of Nominal concentration |
Sampling scheme during the study:
Study date | 0 (a) | 2 (b) | 2 (c) | 9 (a) | 12 (b) | 12 (c) | 19 (a) | 21 (b) | 21 (c) | |
Test group | Nominal concentration [mg/] | Sample Identification Number | ||||||||
0 | 0 (Control) | 201 | 211 | 221 | 231 | 241 | 251 | 261 | 271 | 281 |
1 | 1.56 | 202 | 212 | 222 | 232 | 242 | 252 | 262 | 272 | 282 |
2 | 3.13 | 203 | 213 | 223 | 233 | 243 | 253 | 263 | 273 | 283 |
3 | 6.25 | 204 | 214 | 224 | 234 | 244 | 254 | 264 | 274 | 284 |
4 | 12.5 | 205 | 215 | 225 | 235 | 245 | 255 | 265 | 275 | 285 |
5 | 25 | 206 | 216 | 226 | 236 | 246 | 256 | 266 | 276 | 286 |
Total primary samples | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | |
Total retain samples (d) | 12 | 12 | 12 | 12 | 12 | 12 | 12 | 12 | 12 | |
(a): Fresh test solution | ||||||||||
(b): Aged test solution (48-72 hours old) | ||||||||||
(c): Abiotic replicate (no Daphnia or food algae) | ||||||||||
(d): Retained samples are indicated with a "b" or "c" in addition to the sample number | ||||||||||
Summarized results of the first set of samples:
Sample name | Preparation | Expected content | Found content | Mean content | Mean recovery |
[mg/L] | [mg/L] | [mg/L] (1) | [%](1) | ||
201 (2) | a | 0 | <0.5 | <0.5 | - |
201 (2) | b | 0 | <0.5 | ||
202 (2) | a | 1.56 | 1.65 | 1.63 | 104 |
202 (2) | b | 1.56 | 1.63 | ||
203 (2) | a | 3.13 | 3.27 | 3.26 | 104 |
203 (2) | b | 3.13 | 3.26 | ||
204 (2) | a | 6.25 | 6.54 | 6.48 | 104 |
204 (2) | b | 6.25 | 6.43 | ||
205 (2) | a | 12.5 | 12.7 | 12.8 | 102 |
205 (2) | b | 12.5 | 12.8 | ||
206 (2) | a | 25 | 25 | 25 | 100 |
206 (2) | b | 25 | 25 |
(1) Calculation is based on non-rounded values
(2) Sample production: Sep 22, 2021; Sampling on: Sep 22 2021. Samples were frozen on sampling date and analyzed on Sep 23, 2021.
Summarized results of the second set of samples | |||||
Sample name | Preparation | Expected content | Found content | Mean content | Mean recovery |
[mg/L] | [mg/L] | [mg/L] (1) | [%] (1) | ||
211 (2) | a | 0 | <0.5 | <0.5 | - |
211 (2) | b | 0 | <0.5 | ||
212 (2) | a | 1.56 | 1.55 | 1.55 | 100 |
212 (2) | b | 1.56 | 1.56 | ||
213 (2) | a | 3.13 | 3.12 | 3.12 | 100 |
213 (2) | b | 3.13 | 3.13 | ||
214 (2) | a | 6.25 | 6.03 | 6.12 | 98 |
214 (2) | b | 6.25 | 6.21 | ||
215 (2) | a | 12.5 | 12.4 | 12.4 | 99 |
215 (2) | b | 12.5 | 12.4 | ||
216 (2) | a | 25 | 24 | 24 | 96 |
216 (2) | b | 25 | 24 | ||
221 (2) | a | 0 | <0.5 | <0.5 | - |
221 (2) | b | 0 | <0.5 | ||
222 (2) | a | 1.56 | 1.68 | 1.65 | 106 |
222 (2) | b | 1.56 | 1.61 | ||
223 (2) | a | 3.13 | 3.09 | 3.13 | 100 |
223 (2) | b | 3.13 | 3.17 | ||
24 (2) | a | 6.25 | 6.28 | 6.26 | 100 |
24 (2) | b | 6.25 | 6.24 | ||
25 (2) | a | 12.5 | 12.2 | 12.3 | 98 |
25 (2) | b | 12.5 | 12.5 | ||
26 (2) | a | 25 | 25 | 25 | 99 |
26 (2) | b | 25 | 25 | ||
(1) Calculation is based on non-rounded values | |||||
(2) Sample production: Sep 22, 2021; Sampling on: Sep 24 2021. Samples were frozen on sampling date and analyzed on Sep 29, 2021. |
Summarized results of the third set of samples | |||||
Sampling day | Preparation | Expected content | Found content | Mean content | Mean recovery |
[mg/L] | [mg/L] | [mg/L] (1) | [%](1) | ||
231 (2) | a | 0 | <0.5 | <0.5 | - |
231 (2) | b | 0 | <0.5 | ||
232 (2) | a | 1.56 | 1.39 | 1.40 | 90 |
232 (2) | b | 1.56 | 1.41 | ||
233 (2) | a | 3.13 | 2.85 | 2.87 | 92 |
233 (2) | b | 3.13 | 2.89 | ||
234 (2) | a | 6.25 | 5.75 | 5.66 | 90 |
234 (2) | b | 6.25 | 5.56 | ||
235 (2) | a | 12.5 | 11.6 | 11.6 | 93 |
235 (2) | b | 12.5 | 11.6 | ||
236 (2) | a | 25 | 23 | 24 | 94 |
236 (2) | b | 25 | 24 | ||
(1) Calculation is based on non-rounded values | |||||
(2) Sample production: Oct 01, 2021; Sampling on: Oct 01, 2021 | |||||
Summarized results of the fourth set of samples | |||||
Sample name | Preparation | Expected content | Found content | Mean content | Mean recovery |
[mg/L] | [mg/L] | [mg/L] (1) | [%] (1) | ||
241 (2) | a | 0 | <0.5 | <0.5 | - |
241 (2) | b | 0 | <0.5 | ||
242 (2) | a | 1.56 | 1.47 | 1.46 | 93 |
242 (2) | b | 1.56 | 1.44 | ||
243 (2) | a | 3.13 | 3.10 | 3.09 | 99 |
243 (2) | b | 3.13 | 3.09 | ||
244 (2) | a | 6.25 | 5.91 | 5.86 | 94 |
244 (2) | b | 6.25 | 5.81 | ||
245 (2) | a | 12.5 | 13.1 | 12.9 | 104 |
245 (2) | b | 12.5 | 12.8 | ||
246 (2) | a | 25 | 24 | 24 | 97 |
246 (2) | b | 25 | 24 | ||
251 (2) | a | 0 | <0.5 | <0.5 | - |
251 (2) | b | 0 | <0.5 | ||
252 (2) | a | 1.56 | 1.35 | 1.35 | 87 |
252 (2) | b | 1.56 | 1.35 | ||
253 (2) | a | 3.13 | 2.75 | 2.80 | 89 |
253 (2) | b | 3.13 | 2.84 | ||
254 (2) | a | 6.25 | 5.89 | 5.78 | 93 |
244 (2) | b | 6.25 | 5.67 | ||
255 (2) | a | 12.5 | 11.8 | 11.8 | 94 |
255 (2) | b | 12.5 | 11.8 | ||
256 (2) | a | 25 | 23 | 23 | 93 |
256 (2) | b | 25 | 23 | ||
(1) Calculation is based on non-rounded values | |||||
(2) Sample production: Oct 01, 2021; Sampling on: Oct 04, 2021. Samples were frozen on sampling date and analyzed on Oct 12, 2021 |
Summarized results of the fifth set of samples | |||||
Sampling day | Preparation | Expected content | Found content | Mean content | Mean recovery |
[mg/L] | [mg/L] | [mg/L] (1) | [%](1) | ||
261 (2) | a | 0 | <0.5 | <0.5 | - |
261 (2) | b | 0 | <0.5 | ||
262 (2) | a | 1.56 | 1.56 | 1.56 | 100 |
262 (2) | b | 1.56 | 1.56 | ||
263 (2) | a | 3.13 | 3.11 | 3.08 | 98 |
263 (2) | b | 3.13 | 3.04 | ||
264 (2) | a | 6.25 | 6.17 | 6.09 | 97 |
264 (2) | b | 6.25 | 6.02 | ||
265 (2) | a | 12.5 | 12.5 | 12.3 | 98 |
265 (2) | b | 12.5 | 12.2 | ||
266 (2) | a | 25 | 24 | 24 | 96 |
266 (2) | b | 25 | 24 | ||
(1) Calculation is based on non-rounded values | |||||
(2) Sample production: Oct 11, 2021; Sampling on: Oct 11, 2021 |
Summarized results of the sixth set of samples | |||||
Sample name | Preparation | Expected content | Found content | Mean content | Mean recovery |
[mg/L] | [mg/L] | [mg/L] (1) | [%] (1) | ||
271 (2) | a | 0 | <0.5 | <0.5 | - |
271 (2) | b | 0 | <0.5 | ||
272 (2) | a | 1.56 | 1.52 | 1.52 | 97 |
272 (2) | b | 1.56 | 1.51 | ||
273 (2) | a | 3.13 | 3.04 | 3.05 | 98 |
273 (2) | b | 3.13 | 3.07 | ||
274 (2) | a | 6.25 | 6.66 | 6.58 | 105 |
274 (2) | c | 6.25 | 6.59 | ||
274 (2) | b | 6.25 | 6.50 | ||
275 (2) | a | 12.5 | 12.2 | 12.2 | 98 |
275 (2) | b | 12.5 | 12.3 | ||
276 (2) | a | 25 | 25 | 24 | 97 |
276 (2) | b | 25 | 24 | ||
281 (2) | a | 0 | <0.5 | <0.5 | - |
281 (2) | b | 0 | <0.5 | ||
282 (2) | a | 1.56 | 1.53 | 1.53 | 98 |
282 (2) | b | 1.56 | 1.52 | ||
283 (2) | a | 3.13 | 3.05 | 3.05 | 98 |
283 (2) | b | 3.13 | 3.06 | ||
284 (2) | a | 6.25 | 6.08 | 6.02 | 96 |
284 (2) | b | 6.25 | 5.95 | ||
285 (2) | a | 12.5 | 12.2 | 12.2 | 98 |
285 (2) | b | 12.5 | 12.3 | ||
286 (2) | a | 25 | 25 | 25 | 99 |
286 (2) | b | 25 | 25 | ||
(1) Calculation is based on non-rounded values | |||||
(2) Sample production: Oct 11, 2021; Sampling on: JOct 13, 2021 |
Description of key information
A Daphnia magna reproduction test (semi-static, 21 d) of the test item EDTA-ZnK2 was conducted according to OECD 211 under GLP conditions.
Test species was Daphnia magna STRAUS. Ten daphnids, held individually, were used per nominal test item concentration and control. At the start of the exposure the daphnids were less than 24 hours old. The study was carried out under semi-static conditions with medium exchange three times per week. Aim of the test was to assess the effects on the reproduction capacity and other test item-related effects or parameters such as the intrinsic rate of the natural increase, the number of broods with living juveniles, first appearance of living juveniles, occurrence of aborted eggs and stillborn juveniles, adult mortality, body length and dry weight of the parental daphnids.
The test item EDTA-ZnK2 is a white solid. The aqueous media were prepared dissolving 50 mg of test substance in 2-L of test medium and stirring for approximately 10 min at ambient temperature. The lower test concentrations were prepared by dilution of this stock solution. Before the dilution was performed, the stock solution was checked for complete dissolution of the test substance. Five test concentrations were prepared and tested nominal concentration rates 25 – 12.5 – 6.25 – 3.13 - 1.56 mg//L (separation factor 2.0).
The stock solutions were checked visually to ensure that the test substance was completely dissolved.
The test item concentrations of EDTA-ZnK2 were analytically verified via LC-MS three times during the test (once within a period of 7 days) in the fresh media at the start of an exposure-renewal interval (0 hours; on test days 0, 9 and 19) as well as in the old media at the end of an exposure-renewal interval (24 hours; on test days 2, 12 and 21) in all test item concentrations and in the control.
The environmental conditions were within the acceptable limits. The validity criteria of the test guideline were met.
The OECD 211 test guideline is primarily designed to assess effects on Daphnia reproductive output (living young) as the primary population relevant endpoint. Measuring and reporting other biological parameters such as growth, %immobile young, time to first brood and %aborted eggs is optional. These biological parameters in addition to reproduction may assist in the overall interpretation of the study results; however, they alone are not indicative of a population relevant effect. A statistically significant difference in these additional parameters without an effect on reproduction is not considered in establishing the NOEC. No significant mortality or any other additional significant adverse effects or abnormal behavior were observed in any of the test treatments. The following overall effect concentrations (mg/L) are based on reproduction (living offspring per surviving adult) as the primary population relevant response and were obtained based on nominal concentrations:
LOEC = > 25 mg/L
NOEC = ≥ 25 mg/L
Since the measured concentrations of the test substance in the test solutions were within +20% of the nominal concentrations, the effect concentration can be expressed relative to the nominal concentration.
The purity of the test substance used in the test was 90.4%
LOEC = > 25 mg test substance/L or 22.6 mg a.i./L
NOEC = ≥ 25 mg test substance/L or 22.6 mg a.i./L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 22.6 mg/L
Additional information
Three reliable 21 d chronic toxicity studies for daphnids are available. A study from 2022 with EDTA-CuK2 which was performed in parallel to the study with the test substance (EDTA-ZnK2) and gave similar to the study with EDTA-CuK2 a 21d NOEC of 25 mg test substance/L.
There are also two older studies which both gave results in the same order of magnitude. The Key value used in the EU RAR 2004 is a study from BASF performed with EDTA-Na2H2 (CAS no 139-33-3). This study resulted similar as for EDTA-CuK2 and EDTA-ZnK2 in a 21d NOEC of 25 mg/L Recalculation towards EDTA-ZnK2 via MW ratio results in 431.8/336.21 x 25 = 32.1 mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)