Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 264-355-5 | CAS number: 63589-47-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Biodegradation in water
Biodegradability of 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS no. 63589 -47 -9) is predicted using QSAR toolbox version 3.3 (2017) with logKow as the primary descriptor. Test substance undergoes 1.86% degradation by BOD in 28 days. Thus, based on percentage degradation, the test chemical 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) was estimated to be not readily biodegradable in water.
Adsorption / desorption
KOCWIN model (v2.00) of Estimation Programs Interface (EPI Suite, 2017) was used to predict the soil adsorption coefficient i.e Koc value of test chemical 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No. 63589 -47 -9). The soil adsorption coefficient i.e Koc value of 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) was estimated to be 699600 L/kg (log Koc= 5.8448) by means of MCI method (at 25 deg C). This Koc value indicates that the substance 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) has a very strong sorption to soil and sediment and therefore have negligible migration potential to ground water.
Additional information
Biodegradation in water
Various predicted data for the target compound 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No. 63589-47-9) and supporting weight of evidence studies for its closest read across substance with logKow as the primary descriptor were reviewed for the biodegradation end point which are summarized as below:
In a prediction done by SSS (2017) using OECD QSAR toolbox version 3.3 with logKow as the primary descriptor, percentage biodegradability of test chemical 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No. 63589-47-9) was estimated.Test substance undergoes 1.86% degradation by BOD in 28 days. Thus, based on percentage degradation, the test chemical 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) was estimated to be not readily biodegradable in water.
In another prediction using the Estimation Programs Interface Suite (EPI suite, 2017), the biodegradation potential of the test compound 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No. 63589-47-9) in the presence of mixed populations of environmental microorganisms was estimated.The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical 3,7 -bis(diethylamino)phenoxazin-5 -ium tetrachlorozincate (2:1) is expected to be not readily biodegradable.
In a supporting weight of evidence study from peer reviewed journal (H. Heukelekian and M. C. Rand, 1955) for the read across chemical [4-[α-[4-(dimethylamino)phenyl]benzylidene]cyclohexa-2,5-dien-1-ylidene]dimethylammonium chloride (CAS no. 569-64-2),biodegradation experiment was conducted for 5 days for evaluating the percentage biodegradability of read across substance [4-[α-[4-(dimethylamino)phenyl]benzylidene]cyclohexa-2,5-dien-1-ylidene]dimethylammonium chloride using standard dilution method at a temperature of 20°C. Sewage was used as a test inoculum. The 5 day BOD value of chemical [4-[α-[4-(dimethylamino)phenyl]benzylidene]cyclohexa-2,5-dien-1-ylidene]dimethylammonium chloride was determined to be 0 g/g. Thus, based on this value, [4-[α-[4-(dimethylamino)phenyl]benzylidene]cyclohexa-2,5-dien-1-ylidene]dimethylammonium chloride is considered to be not readily biodegradable in nature.
Another biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of the same read across substance [4-[α-[4-(dimethylamino)phenyl]benzylidene]cyclohexa-2,5-dien-1-ylidene]dimethylammonium chloride (CAS no. 569-64-2) (EnviChem, 2014). The study was performed according to OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I). Activated sludge was used as a test inoculums for the study. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of substance [4-[α-[4-(dimethylamino)phenyl]benzylidene]cyclohexa-2,5-dien-1-ylidene]dimethylammonium chloride was determined to be 0.3% by BOD parameter in 14 days. Thus, based on percentage degradation, [4-[α-[4-(dimethylamino)phenyl]benzylidene]cyclohexa-2,5-dien-1-ylidene]dimethylammonium chloride is considered to be not readily biodegradable in nature.
For the read across chemical sodium 4-[3,6-bis(diethylamino)-2,7-dimethylxanthenium-9-yl]benzene-1,3-disulfonate (CAS no. 3520-42-1) from authoritative database (J-CHECK, 2016), biodegradation experiment was conducted for 28 days for evaluating the percentage biodegradability of read across substance sodium 4 -[3,6 -bis(diethylamino)-2,7 -dimethylxanthenium-9 -yl]benzene-1,3 -disulfonate. The study was performed according to OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I) and other guideline “Biodegradation test of a chemical substance using a microorganism etc.” provided in “the Notice on the Test Method Concerning New Chemical Substances", respectively. Concentration of inoculum i.e, activated sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of substance was determined to be 6, 0 and 0% by O2 consumption, TOC removal, Test mat. analysis and HPLC parameter in 28 days. Thus, based on percentage degradation, sodium 4 -[3,6 -bis(diethylamino)-2,7 -dimethylxanthenium-9 -yl]benzene-1,3 -disulfonate is considered to be not readily biodegradable in nature.
In a supporting weight of evidence study from authoritative database (J-CHECK, 2016) for the read across chemical Sodium 3-({[4-([4-(dimethylamino)phenyl]{4-[ethyl(3-sulfonatobenzyl)amino]phenyl}methylene)cyclohexa-2,5-dien-1-ylidene](ethyl)ammonio}methyl)benzenesulfonate (CAS no. 1694-09-3), biodegradation experiment was conducted for 28 days for evaluating the percentage biodegradability of read across substance sodium 3 -({[4 -([4 -(dimethylamino)phenyl]{4 -[ethyl(3 -sulfonatobenzyl)amino]phenyl}methylene)cyclohexa-2,5 -dien-1 -ylidene](ethyl)ammonio}methyl)benzenesulfonate. Concentration of inoculum i.e, activated sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of substance was determined to be 1, 4 and 0% by BOD, TOC removal and HPLC parameter in 28 days. Thus, based on percentage degradation, 3 -({[4 -([4 -(dimethylamino)phenyl]{4 -[ethyl(3 -sulfonatobenzyl)amino]phenyl}methylene)cyclohexa-2,5 -dien-1-ylidene](ethyl)ammonio}methyl) benzenesulfonate is considered to be not readily biodegradable in nature.
On the basis of above results for target chemical 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (from OECD QSAR toolbox version 3.3 and EPI suite, 2017) and for its read across substance (from peer reviewed journal and authoritative database J-CHECK & EnviChem), it can be concluded that the test substance 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) can be expected to be not readily biodegradable in nature.
Adsorption / desorption
KOCWIN model (v2.00) of Estimation Programs Interface (EPI Suite, 2017) was used to predict the soil adsorption coefficient i.e Koc value of test chemical 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) (CAS No. 63589 -47 -9). The soil adsorption coefficient i.e Koc value of 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) was estimated to be 699600 L/kg (log Koc= 5.8448) by means of MCI method (at 25 deg C). This Koc value indicates that the substance 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1) has a very strong sorption to soil and sediment and therefore have negligible migration potential to ground water.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.