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EC number: 259-393-4 | CAS number: 54914-37-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2011-07-11 to 2011-07-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mg/L
- Sampling method: Samples taken from test container (aquaria)
- Sample storage conditions before analysis: Samples were taken, immediately transferred to the analytical laboratory and analysed on the same day. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Test substance was directly weighed into the test vessels after adding the test water.
- Two barrels (30 L) were prepared: one was filled with 10.5 L of dilution wtaer for the control. The 2nd barrel was also filled with 10.5 L dilution water, stirred with a magnetic stirrer (300 rpm) and 1050 mg test substance were added (= 100 mg/L). The test medium was stirred for 24 h. Afterwards, undissolved particles were removed by filtration
- Controls: control preparation without test substance was treated the same way.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): precipitate removed by filtration. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebrafish
- Strain: Hamilton-Buchanan
- Source: Raiffeisenmarkt, 47906 Kempen, Germany
- Length at study initiation (length definition, mean, range and SD): 2 +/- 1 cm
- Feeding during test: There was no feeding throughout the exposure period in the test.
ACCLIMATION
- Acclimation period: at least 12 days
- Acclimation conditions: in 300 L glass tanks, illumination 12/12 hours light/dark cycle, aeration with compressed oil free air (at least 80% air saturation)
- Type and amount of food: The fish were fed with “Tetra Min” dry food from Tetra, Melle, Germany.
- Feeding frequency: daily
- Health during acclimation (any mortality observed): Their state of health was controlled routinely during holding.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 1.818 mmol/L (total hardness)
- Test temperature:
- 21.7 - 22.2 °C
- pH:
- 8.01 - 8.68
- Dissolved oxygen:
- 71.0 - 76.8 %
- Nominal and measured concentrations:
- nominal (measured DOC at 0 h in brackets): 100 mg/L ( 53.7 mg/L)
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: all glas aquaria, 15 L volume, filled with 10.5 L of test solution
- Aeration: dilution water was aerated before use in the experiment
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The holding and dilution water for the fish was prepared separately in a silicone sealed PVC tank (250 L) by mixing demineralised water with de-chlorinated tap water from municipal source (SWK Aqua GmbH, Krefeld, Germany) in a ratio of 3:2 and 9 mL of sulphuric acid.
- Total organic carbon: 0 mg/L
- Alkalinity: 0.75 mmol/L
- Conductivity: 358 µS/cm
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Photoperiod: 12 h / 12 h light / dark cycle
EFFECT PARAMETERS MEASURED : mortality, symptoms of toxicity at 0, 24, 48, 72 and 96 h
- Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 53.7 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- dissolved
- Remarks:
- (DOC)
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 53.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Remarks:
- (DOC)
- Basis for effect:
- mortality (fish)
- Details on results:
- No mortality was observed during the study. Fish did not show any symptoms of toxicity.
- Reported statistics and error estimates:
- Statistical analysis was done with ToxRat Professional (ToxRat Solutions GmbH, Alsfeld, Germany)
- Sublethal observations / clinical signs:
Summary of mortality data
Nominal Concentration of test substance
Number of dead fish
Exposure period
Cumulative frequency (%)
Exposure period
[mg/L]
3 h
6 h
24 h
48 h
72 h
96 h
3 h
6 h
24 h
48 h
72 h
96 h
(0) Control
0
0
0
0
0
0
0
0
0
0
0
0
100
0
0
0
0
0
0
0
0
0
0
0
0
Analytical measurements
Time
Control (dilution water)
100 mg/L
TC (mg/L)
TIC
(mg/L)
DOC1
(mg/L)
TC (mg/L)
TIC
(mg/L)
DOC
(mg/L)2
Recovery in %
0h
11.4
11.3
0.1
69.2
15.4
53.7
24 h
11.4
11.4
≤0
67.3
17.1
50.2
93
48 h
11.4
11.5
≤0
66.3
17.2
49.1
91
72 h
11.3
11.9
≤0
72.0
17.5
54.5
101
96 h
11.4
11.8
≤0
70.8
17.7
53.1
99
1DOC = TC – TIC
2DOC = DOC minus DOC Control
Recovery rate for DOC in % after 24h, 48h, 72h and 96h relating to 0h values.
Percent values were rounded.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50 (96 h)of based on nominal concentration is > 100 mg/L and based on the measured DOC >53.7 mg/L and indicating that the test substance does not pose a hazard to fish.
- Executive summary:
The test substance was tested in the Fish Acute Toxicity Test according to the OECD Guideline for the Testing of Chemicals No. 203 (July 1992) using the zebra fish, Danio rerio (Hamilton-Buchanan) as test organism. The experiment was carried out as a static limit test, i.e. the test substance preparations were not exchanged for the whole exposure period of 96 hours. The results of this study show that the test substance does not have any impact on Danio rerio at the tested concentration of 100 mg/L (nominal). Hence the LC50value after 96 h of exposure was determined a >100 mg/L, based on nominal concentration. Based on the measured DOC value the LC50 value after 96 h was > 53.7 mg/L.
Reference
Description of key information
The test substance was tested in the Fish, Acute Toxicity Test according to the OECD Guideline for the Testing of Chemicals No. 203 (July 1992) using the zebra fish, Danio rerio (Hamilton-Buchanan) as test organism. The experiment was carried out as a static limit test, i.e. the test substance preparations were not exchanged for the whole exposure period of 96 hours. The results of this study show that the test substance does not have any impact on Danio rerio at the tested concentration of 100 mg/L (nominal). Hence the LC50 value after 96 h of exposure was determined a >100 mg/L, based on nominal concentration. Based on the measured DOC value the LC50 value after 96 h was > 53.7 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- EC50
- Effect concentration:
- 53.7 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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