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EC number: 254-663-8 | CAS number: 39872-57-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2022-06-16 to 2022-08-15
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal sewage treatment plant, Hildesheim, Germany.
- Pretreatment: The activated sludge was washed twice with chlorine free tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration with CO2 free air until test start (3 days) at the test temperature. The amount of inoculum used to initiate inoculation was 4.76 mL/L (25.0 mg/L dw). - Duration of test (contact time):
- 60 d
- Initial conc.:
- 19 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral salts medium according to OECD 301 F
- Test temperature: Nominal: 20 - 24 ± 1 °C; Actual: 22.1 – 22.5°C
- Photoperiod: Dark
TEST SYSTEM
- Culturing apparatus: Closed bottles
- Number of culture flasks/concentration: Total number of replicates of test item 2, total number of replicates of inoculum control 4, total number of replicates of functional control 2, total number of replicates of toxicity control 1.
- Measuring equipment: The oxygen consumption was determined in the incubation vessels by the OxiTop® measuring system at 360 measuring points during the 60 d incubation period.
- Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 41
- Sampling time:
- 60 d
- Details on results:
- Degradation kinetics (%):
-Day 14: Rep1: 1%, Rep2: 0%
-Day 28: Rep1: 1%, Rep2: 12%
-Day 42: Rep1 6%, Rep2: 28%
-Day 60: Rep1: 28%, Rep2 53%
The biodegradation of the test item begins with a certain lag time (approx. 24 -36 days). Because of the late start of the biodegradation, special bacterial cultures are probably required, which consequently generate a deviation between the test item replicates. Since the two test item replicates show an identical increase in percentage biodegradation and the first replicate only starts with a delay, the deviations between the two test item replicates are considered to be acceptable.
Toxicity control after 14 days: 39% (it is assumed that the test item is not inhibitory). After 28 days: 50%. After 60 days: 82%. - Results with reference substance:
- Biodegradation:
- Day 14: Rep1: 80%, Rep2: 90%
- Day 28: Rep1: 90%, Rep2: 100%
- Day 42: Rep1: 93%, Rep2: 100%
- Day 60: Rep1: 93%, Rep2: 100% - Validity criteria fulfilled:
- yes
- Remarks:
- see details on results
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The study showed that the test item is not readily and not inherently, ultimately biodegradable as it did not reach the respective pass pass levels (60% of biodegradability).
- Executive summary:
The present study was conducted in order to evaluate the biodegradability of the test item by measuring its percentage of biodegradation with inoculum in a period of 60 days. The test method was performed following the OECD Test Guideline 301F and GLP. The test item concentration was 19 mg/L. Benzoic acid was used as reference substance in the functional control as well as in the toxicity control.
The biodegradation of the test item begins with a certain lag time (approx. 24 -36 days). Because of the late start of the biodegradation, special bacterial cultures are probably required. The two test item replicates show an identical increase in percentage biodegradation, but the first replicate starts with a delay (10% reached on day 46, compared to 10% reached on day 27 for replicate 2). After the 60-d period the mean biodegradation of the test item was calculated to be 41% without achieving a plateau (replicate 1: 28%; replicate 2: 53%). The study shows that the test item degradation starts after a long-lag phase (> 26 days) and would degrade even more over a longer time. Nevertheless, the test item has to be regarded as not readily and not inherent, ultimately biodegradable, but can be classified as inhernt, primarily biodegradable.
Reference
Description of key information
Not readily biodegradable: 41% after 60d (OECD 301)
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
Additional information
The present study was conducted in order to evaluate the biodegradability of the test item by measuring its percentage of biodegradation with inoculum in a period of 60 days. The test method was performed following the OECD Test Guideline 301F and GLP. The test item concentration was 19 mg/L. Benzoic acid was used as reference substance in the functional control as well as in the toxicity control.
The biodegradation of the test item begins with a certain lag time. Because of the late start of the biodegradation, special bacterial cultures are probably required. The two test item replicates show an identical increase in percentage biodegradation, but the first replicate starts with a delay (10% reached on day 46, compared to 10% reached on day 27 for replicate 2). After the 60-d period the mean biodegradation of the test item was calculated to be 41% without achieving a plateau (replicate 1: 28%; replicate 2: 53%). The study shows that the test item degradation starts after a long-lag phase and would degrade even more over a longer time. Nevertheless, the test item has to be regarded as not readily and not inherent, ultimately biodegradable, but can be classified as inhernt, primarily biodegradable.
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