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EC number: 247-956-7 | CAS number: 26748-47-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000-02-22 to 2000-08-21
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- batch number: protocol and labelling: 860-9806-703
description: translucent yellow liquid
composition: peroxide content: approximately 70%
isododecane content: approximately 30%
density: 0.921 at 7°C
container: one plastic flask
date of receipt: 1 December 1999
storage conditions: at -20°C. - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Each concentration was prepared separately by adding 16 mL of sewage feed to each of the flasks which were made up to 300 mL with water only in the case of the controls or a nominal mixture of water and test substance (added directly using a micropipette or a syringue for microinjections) in the case of the test solutions. Each mixture was then made up to 500 mL by adding 200 mL of inoculum. The solutions were aerated at between 0.5 to 1 litre of air per minute using a Pasteur-pipette as an aeration device. Solutions were prepared approximately every 15 minutes for test times of 3 hours. In order to verify the quality of the inoculum, the controls were prepared at the beginning (first control) and the end (second control) of the test. After 3 hours of aeration/contact time, the content of the test flask was poured into the measuring apparatus and the oxygen concentration was determined during a few minutes.
Groups of test and reference solutions and controls were set up as follows:
- two controls containing inoculum at 1.6 g/L,
- three test concentrations containing CUMYLPEROXYNEODECANOATE at 2, 10 and 20 mg/L and inoculum at 1.6 g/L,
- three reference concentrations containing the reference substance (3,5-Dichlorophenol) at 4,12 and 36 mg/L and inoculum at 1.6 g/L.
These solutions were prepared in test flasks and then immediately aerated for 3 hours before being transferred to an oxygen measuring apparatus.
Measurement of oxygen concentration of the above test solutions was determined using an oxygen probe placed into an opaque BOD flask filled with the test or reference solution which was agitated with a magnetic stirrer. The oxygen probe was connected to a meter and thence to a chart recorder. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: No
- Name and location of sewage treatment plant where inoculum was collected: Emeraude (SIARR) (76141 Petit-Quevilly, France).
- Method of cultivation: The inoculum was left to settle and the supernatant rejected. It was then filtered (to remove the biggest particules) and washed with dechlorinated water.
- Pre-treatment: The inoculum was maintained under agitation for one day before the test. As the sludge was not used immediately, 50 ml of sewage feed were added, per litre, just before agitation. Synthetic sewage feed was prepared using dechlorinated water and analytical grade reagents following OECD recommendations.
Temperature: between 22 °C and 23 °C.
Illumination: the stock vessel was kept away from light by wrapping it in opaque material.
Aeration: clean air was passed through the inoculum at the rate of 0.5 to 1 L/min until use.
pH: was checked before use. The inoculum was buffered, if necessary, to pH 6.0 to 8.0 using sodium bicarbonate solution.
- Initial biomass concentration: Four samples of 50 mL were taken to determine the content in suspended matter (dry weight). The concentration of sludge (6.74 g/L) was then adjusted to 4 g/L by addition of the appropriate volume of dechlorinated water. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- 280 ± 20 mg/L as CaCO3.
- Test temperature:
- 22 - 23 °C
- pH:
- 6.0 - 8.0
- Dissolved oxygen:
- Not reported in detail: Air was bubbled through each solution at the rate of 0.5 to 1 L/min until the start of oxygen concentration measurements.
- Nominal and measured concentrations:
- Nominal concentrations: 2, 10, 20 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: BOD flask
- Aeration: yes, air was bubbled through each solution at the rate of 0.5 to 1 L/min until the start of oxygen concentration measurements.
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 2
- No. of vessels per reference control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic sewage feed was prepared using dechlorinated water and analytical grade reagents following OECD recommendations.
OTHER TEST CONDITIONS
- Adjustment of pH: pH was checked before use. The inoculum was buffered, if necessary, to pH 6.0 to 8.0 using sodium bicarbonate solution.
- Illumination: the stock vessel was kept away from light by wrapping it in opaque material
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Respiration rate - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 20 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 20 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- The respiration rate of the test solution at the highest concentration in cumylperoxyneodecanoate (20 mg/L) was equivalent to the respiration rate of the first control (i.e. these rates were within 15% of each other). Therefore, the oxygen consumption rate of the two other test solutions was not determined.
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes, the difference between the two controls was below 15% and the EC50 of 3,5-dichlorophenol was between 5 and 30 mg/L
- Relevant effect levels: EC50 of 3,5-dichlorophenol: 10 mg/L (95% C.I.: 9-12) - Reported statistics and error estimates:
- When for at least two concentrations, inhibition is > 0 % and < 100 %, the EC50 is calculated according to Probit analysis (i.e. Finney’s method, published by E. Weber, combined with Bliss’s method). The confidence interval limits are calculated statistically according to Fieller’s method.
When at only one concentration, inhibition is > 0 % and < 100 %, the EC50 is also calculated by Probit analysis. In this case, the highest concentration causing no inhibition and the lowest concentration producing 100 % inhibition are used as confidence limits.
If at all concentrations inhibition is 0 % or 100 %, the EC50 corresponds to the geometric mean of the highest concentration causing no inhibition and the lowest concentration producing 100 % inhibition. - Validity criteria fulfilled:
- yes
- Conclusions:
- An activated sludge respiration inhibition test according to OECD 209 resulted in an EC50 > 20 mg/L. The test substance did not inhibit the respiration of the activated sludge. Cumylperoxyneodecanoate (70 % in isododecane) is not toxic to aerobic microorganisms in sewage sludge.
- Executive summary:
The effect of cumylperoxyneodecanoate (70 % in isododecane) on respiration of activated sewage sludge was evaluated using a 3 -hour static test according OECD guideline 209. No inhibition (0 %) of the respiration rate was noted at 2, 10 and 20 mg/L of cumylperoxyneodecanoate, relative to mean of the controls. The 3-hour EC50 of the test item for activated sludge is > 20 mg/L. As the validity criteria were met (the difference between the two controls was below 15 % and the EC50 of the reference substance 3,5-dichlorophenol was between 5 and 30 mg/L. The test is considered valid.
Reference
The respiration rate of the test solution at the highest concentration in cumylperoxyneodecanoate (20 mg/L) was equivalent to the respiration rate of the first control (i.e. these rates were within 15 % of each other). Therefore, the oxygen consumption rate of the two other test solutions was not determined.
EC50s, based on nominal concentrations, were as follows:
Substance | 3-hour EC50 (mg/L) | 95% confidence limits (mg/L) |
Test substance | > 20 | |
Reference substance | 10 | 9 - 12 |
Based on the inhibition percentage of the respiration rate obtained in the test solution of highest concentration, the 3-hour EC10 of the test substance was also > 20 mg/L
Description of key information
A respiration inhibition test with activated sludge according OECD guideline 209 was conducted by L´Haridon (2000) to determine the toxicity of 1-methyl-1-phenylethyl peroxyneodecanoate (70% in isododecane) towards microorganisms. The respiration rate of the test solution at the highest test concentration of 20 mg/L (nominal) was equivalent to the respiration rate of the control.
This result can be used for the registration of pure 1-methyl-1-phenylethyl peroxyneodecanoate after backcaluclation to the content of 1-methyl-1-phenylethyl peroxyneodecanoate in isododecane. The NOEC for pure 1-methyl-1-phenylethyl peroxyneodecanoate is 14 mg/L.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 14 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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