Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 244-034-6 | CAS number: 20780-49-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- experimental data of read across substances
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on two toxicity to micro-organism studies as-
2. and 3. - GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Details on sampling:
- 2. and 3. - Sampling method: Test chemical of known concentration was prepared in sterile double distilled water.
- Vehicle:
- not specified
- Details on test solutions:
- 2. and 3.
- Preparation of inoculum for exposure: Keep stock cultures of the test strain, Pseudomonas putida, on the nutrient for stock and preliminary cultures in agar slant tubes. Prepare, for onward culturing of the test strain, new stock cultures at intervals of 1 week each. Incubate the inoculated stock cultures at 25"C for 24 h and keep in stock. stock.
If needed, prepare preliminary cultures from stock cultures on the above mentioned nutrient medium in agar slant tubes and incubate at 25°C for 24 h. Then, wash off the cell material with sterile saline. Determine the extinction of the monochromatic radiation at 436nm for a 10ram layer of the bacterial suspension by photoelectric measurement. - Test organisms (species):
- Pseudomonas putida
- Test type:
- not specified
- Water media type:
- freshwater
- Total exposure duration:
- 16 h
- Test temperature:
- 25°C
- Details on test conditions:
- 2. and 3.
TEST SYSTEM
- Test vessel: Erlenmeyer flask
- Type (delete if not applicable): Erlenmeyer flask stoppered with cottoned lined plastic caps was used for the study.
- Material, size, headspace, fill volume: 300 ml Erlenmeyer flask
GROWTH MEDIUM
- Standard medium used: No
- Detailed composition if non-standard medium was used: Composition of test medium contains sodium nitrate (1.06 g), dipotassium hydrogen phosphate (0.6 g), magnesium sulphate (0.2 g), D (+) glucose (10 g), Difco bacto agar (18 g), ferrous sulphate (0.001 g), and 1.5 ml trace element solution.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: After termination of the test period measure the extinction of the monochromatic radiation at 436 nm in a 10-mm layer in the inoculated dilution series. - Reference substance (positive control):
- not specified
- Duration:
- 16 h
- Dose descriptor:
- EC0
- Effect conc.:
- 115 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: 2. No effect were observed on multiplication of bacterial cells.
- Duration:
- 16 h
- Dose descriptor:
- EC0
- Effect conc.:
- 83 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: 3. No effect were observed on multiplication of bacterial cells.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the EC0 value the test chemical on the growth rate, i.e, multiplication of the test organism Pseudomonas putida can be expected to be ranges from 83 to 115 mg/l, respectively.
- Executive summary:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxic effect of the test chemical on micro-organisms.The studies are as mentioned below:
Short term toxicity to Pseudomonas putidastudy was carried out for 16 hr.The study was based on the effects of the test chemical on Pseudomonas putida at a temperature of 25ᵒC. Test chemical of known concentration was prepared in sterile double distilled water. Composition of test medium contains sodium nitrate (1.06 g), dipotassium hydrogen phosphate (0.6 g), magnesium sulphate (0.2 g), D (+) glucose (10 g), Difco bacto agar (18 g), ferrous sulphate (0.001 g), and 1.5 ml trace element solution. Pseudomonas putida was used as a test organism. Keep stock cultures of the test strain,Pseudomonas putida, on the nutrient for stock and preliminary culturesin agar slant tubes. Prepare, for onward culturingof the test strain, new stock cultures at intervals of1 week each. Incubate the inoculated stock cultures at 25"C for 24 h and keep in stock. stock. If needed, prepare preliminary cultures from stock cultures on the above mentioned nutrient medium in agar slant tubes and incubate at 25°C for 24 h. Then, wash off the cell material with sterile saline. Determine the extinction of the monochromatic radiation at 436nm for a 10 mm layer of the bacterial suspension by photoelectric measurement.Erlenmeyer flask of 300 ml stoppered with cottoned lined plastic caps was used for the study.Prepare dilution series in the test vessel.Each of the dilutions contains 1 part v/v of the pollutant solution in 20 to 214 parts v/v of mixture. Prepare the dilution series as follows: the first flask of each series contains 160ml of pollutant solution at the start. Starting from this flask prepare the subsequent dilution stein at a constant dilution ratio by consistently mixing 80ml of preliminary pollutant dilution and 8O ml double distilled water. Consequently, each flask contains 80 ml of culture liquid at the start. Make up each flask of the three dilution series to be inoculated to 100 ml by adding 5 ml each of stock solution I, 5 ml each of stock solution IIand 10 ml each ofthe preparedbacterial suspensionfrom the preliminary culture having a known adjusted extinction value. Leave both inoculated and non-inoculated dilutionseries at 25°C for 16 h. After termination of the test period measure the extinction of the monochromatic radiation at 436 nm in a 10-mm layer in the inoculated dilution series. On the basis of the effect of test chemical on growth rate, i.e, multiplication of the test organism Pseudomonas putida, the 16 h EC0 value was determined to be 115 mg/l.
For the test chemical, another toxicity study to micro-organisms was carried out according to the same procedure as mentioned above. In this study, on the basis of the effect of test chemical on growth rate, i.e, multiplication of the test organism Pseudomonas putida, the 16 h EC0 value was determined to be 83 mg/l.
On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the EC0 value the test chemical on the growth rate, i.e, multiplication of the test organism Pseudomonas putida can be expected to be ranges from 83 to 115 mg/l, respectively.
Reference
Description of key information
On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the EC0 value the test chemical on the growth rate, i.e, multiplication of the test organism Pseudomonas putida can be expected to be ranges from 83 to 115 mg/l, respectively.
Key value for chemical safety assessment
Additional information
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxic effect of the test chemical on micro-organisms. The studies are as mentioned below:
Short term toxicity to Pseudomonas putida study was carried out for 16 hr. The study was based on the effects of the test chemical on Pseudomonas putida at a temperature of 25ᵒC. Test chemical of known concentration was prepared in sterile double distilled water. Composition of test medium contains sodium nitrate (1.06 g), dipotassium hydrogen phosphate (0.6 g), magnesium sulphate (0.2 g), D (+) glucose (10 g), Difco bacto agar (18 g), ferrous sulphate (0.001 g), and 1.5 ml trace element solution. Pseudomonas putida was used as a test organism. Keep stock cultures of the test strain, Pseudomonas putida, on the nutrient for stock and preliminary cultures in agar slant tubes. Prepare, for onward culturing of the test strain, new stock cultures at intervals of1 week each. Incubate the inoculated stock cultures at 25"C for 24 h and keep in stock. stock. If needed, prepare preliminary cultures from stock cultures on the above mentioned nutrient medium in agar slant tubes and incubate at 25°C for 24 h. Then, wash off the cell material with sterile saline. Determine the extinction of the monochromatic radiation at 436nm for a 10 mm layer of the bacterial suspension by photoelectric measurement. Erlenmeyer flask of 300 ml stoppered with cottoned lined plastic caps was used for the study. Prepare dilution series in the test vessel. Each of the dilutions contains 1 part v/v of the pollutant solution in 20 to 214 parts v/v of mixture. Prepare the dilution series as follows: the first flask of each series contains 160ml of pollutant solution at the start. Starting from this flask prepare the subsequent dilution stein at a constant dilution ratio by consistently mixing 80ml of preliminary pollutant dilution and 8O ml double distilled water. Consequently, each flask contains 80 ml of culture liquid at the start. Make up each flask of the three dilution series to be inoculated to 100 ml by adding 5 ml each of stock solution I, 5 ml each of stock solution II and 10 ml each of the prepared bacterial suspension from the preliminary culture having a known adjusted extinction value. Leave both inoculated and non-inoculated dilution series at 25°C for 16 h. After termination of the test period measure the extinction of the monochromatic radiation at 436 nm in a 10-mm layer in the inoculated dilution series. On the basis of the effect of test chemical on growth rate, i.e, multiplication of the test organism Pseudomonas putida, the 16 h EC0 value was determined to be 115 mg/l.
For the test chemical, another toxicity study to micro-organisms was carried out according to the same procedure as mentioned above. In this study, on the basis of the effect of test chemical on growth rate, i.e, multiplication of the test organism Pseudomonas putida, the 16 h EC0 value was determined to be 83 mg/l.
On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the EC0 value the test chemical on the growth rate, i.e, multiplication of the test organism Pseudomonas putida can be expected to be ranges from 83 to 115 mg/l, respectively.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.