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EC number: 237-066-7 | CAS number: 13598-36-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Acute oral toxicity: LD50 1580 mg/kg bw for males and 1560 mg/kg bw for females
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January 1977
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study can be compared to the deleted OECD 401 guideline for testing acute oral toxicity. The study was not performed under GLP.
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- yes
- Remarks:
- number of animals and number of doses
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
- Species:
- rat
- Strain:
- other: Charles River CD strain
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: no data
- Age at study initiation: no data
- Weight at study initiation: 360-440 g (males) and 215-300 g (females)
- Fasting period before study: overnight
- Housing: 3 rats per cage, in suspended plastic cages in a barrier-maintained room
- Diet: Spratts No. 2 autoclaved small animal diet
- Water: ad libitum
- Acclimation period: no data
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 ± 2
- Humidity (%): ca. 50%
- Air changes (per hr): 10
- Photoperiod (hrs dark / hrs light): no data
IN-LIFE DATES: no data - Route of administration:
- oral: gavage
- Vehicle:
- other: de-ionised water
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 10% w/w
MAXIMUM DOSE VOLUME APPLIED: The dose-volume was varied according to the dose level - Doses:
- 1360, 1520, 1700, 1910, 2140, 2390, 2680, and 3000 mg/kg bw
- No. of animals per sex per dose:
- 6
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, gross pathology - Statistics:
- The Oral Median Lethal Dose (LD50) and its 95% Confidence Limits were determined using the method of Weil.
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- ca. 1 580 mg/kg bw
- 95% CL:
- >= 1 470 - <= 1 690
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- ca. 1 560 mg/kg bw
- 95% CL:
- >= 1 440 - <= 1 690
- Mortality:
- At 1360 mg/kg: no mortality occurred.
At 1520 mg/kg: both males and females 4 out of 6 (4/6) died within 2 days after dosing.
At 1700 mg/kg: males 3/6 and females 4/6 died within 3 days after dosing.
At 1910, 2140, and 2390 mg/kg: all 6 males and females 5/6 died within 2-4 days after dosing.
At 2680 and 3000 mg/kg: all animals died within 1 day after dosing. - Clinical signs:
- other: At all dose levels lethargy and piloerection were seen. Red nasal encrustation, loose faeces and ataxia were also seen at dose levels of 1700 mg/kg and above. Brown staining around the mouth was seen at dose levels of 2390 mg/kg and above, while prostrati
- Gross pathology:
- Animals which died during the study:
- Lung: dark red/pink and firm to the touch.
- Stomach: contents – brown/black fluid. The stomach lining appeared ulcerated at dose levels of 1520 mg/kg and above.
- Intestines: contents - brown/black fluid. The lining appeared hyperaemic.
- Spleen: 3000 mg/kg - darker than normal. All other dose levels – paler than normal.
- Thymus: darker than normal.
- Kidneys: paler than normal.
- Liver: paler than normal.
It was noted that organs had a grey/brown discolouration where they came in contact with the stomach, intestines or liver, which, themselves, were similarly discoloured.
Animals killed at the end of the 14-day observation period:
- Liver, kidneys, and spleen: paler than normal, especially where they were in contact with the gastro-intestinal tract.
- Lungs: deeper pink than normal. - Other findings:
- - Other observations: The brown/black fluid observed in the gastro-intestinal tract of animals which died is possibly franked blood and may be indicative of haemorrhage. The effect on the organs surrounding the stomach, as indicated by the grey/brown discolouration, may have been a direct result of the acidity of the compound.
- Interpretation of results:
- harmful
- Remarks:
- Migrated information if swallowed Criteria used for interpretation of results: EU
- Conclusions:
- The oral median lethal dose ( LD50) of 10% Phosphorous acid (w/w) in water was determined in rats. The LD50 and its 95% confidence limits was 1580 (1470-1690) mg/kg for male rats and 1560 (1440-1690) mg/kg for female rats. Based on these results and according to the EU classification criteria outlined in 67/548/EEC and 1272/2008 the compound needs to be classified as harmful if swallowed.
- Executive summary:
- Male and female albino rats of the Charles River CD strain were treated with 10% Phosphorous acid by oral gavage administration at 1360, 1520, 1700, 1910, 2140, 2390, 2680, and 3000 mg/kg bw. The rats were observed for 14 days following dosing after which surviving animals were sacrificed and examined macroscopically. Mortality and clinical signs were recorded. At 1360 mg/kg: no mortality occurred. At 1520 mg/kg: both males and females 4 out of 6 (4/6) died within 2 days after dosing. At 1700 mg/kg: males 3/6 and females 4/6 died within 3 days after dosing. At 1910, 2140, and 2390 mg/kg: all 6 males and females 5/6 died within 2-4 days after dosing. At 2680 and 3000 mg/kg: all animals died within 1 day after dosing. At all dose levels lethargy and piloerection were seen. Red nasal encrustation, loose faeces and ataxia were also seen at dose levels of 1700 mg/kg and above. Brown staining around the mouth was seen at dose levels of 2390 mg/kg and above, while prostration and convulsion were seen only in rats receiving 3000 mg/kg. The time of onset of clinical signs was dose related and varied between 10 minutes after dosing at 3000 mg/kg and 1-17 hours after dosing at the three lower dose levels. All surviving animals had completely recovered by 48 hours after dosing. The brown/black fluid observed in the gastro-intestinal tract of animals which died is possibly franked blood and may be indicative of haemorrhage. The effect on the organs surrounding the stomach, as indicated by the grey/brown discolouration, may have been a direct result of the acidity of the compound. The Oral Median Lethal Dose (LD50) and its 95% confidence limits were determined using the method of Weil.There was no observable difference between the LD50 values obtained in males and females.The LD50 and its 95% confidence limits was 1580 (1470-1690) mg/kg for male rats and 1560 (1440-1690) mg/kg for female rats. Based on these results and according to the EU classification criteria outlined in 67/548/EEC and 1272/2008 the compound needs to be classified as harmful if swallowed.
Reference
There is no observable difference between the LD50 values obtained in males and females.
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 1 560 mg/kg bw
Acute toxicity: via inhalation route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Acute toxicity: via dermal route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
In an acute oral toxicity study, which was performed similar to OECD Guidance 401, male and female albino rats of the Charles River CD strain were treated with 10% Phosphorous acid by oral gavage administration at 1360, 1520, 1700, 1910, 2140, 2390, 2680, and 3000 mg/kg bw. The LD50 and its 95% confidence limits were 1580 (1470-1690) mg/kg for male rats and 1560 (1440-1690) mg/kg for female rats. Based on these results and according to the EU classification criteria outlined in 67/548/EEC and 1272/2008 the compound needs to be classified as harmful if swallowed.
As the substance is corrosive to the skin, the acute dermal and acute inhalation toxicity studies are waived, in accordance with Column 2 of REACH Annex VIII.
Justification for selection of acute toxicity – oral endpoint
One study available which was performed similar to the OECD guidelines.
Justification for classification or non-classification
Based on the available information, phosphonic acid needs to be classified for Acute Oral Toxicity Category 4 in accordance with the criteria outlined in Annex VI of 67/548/EEC and Annex I of 1272/2008/EC.
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