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EC number: 219-143-7 | CAS number: 2372-21-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experimental starting date: 21-10-2014 Experimental completion date: 22-10-2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Chemical name Tert-Butylperoxy isopropyl carbonate
EC name O,O-tert-butyl isopropyl monoperoxycarbonate
Batch/Lot no. 1203442069
Appearance Clear liquid
Solubility Miscible in water (water solubility 2.2 g/L)
Volatile No (vapour pressure 200 Pa, 38°C)
Stability Unstable under test conditions
Storage -20°C in the dark - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test substance was pipetted into the incubation vessels and the added weight was directly measured on an analytical mass balance. The weight of the test substance volumes pipetted in the 1 L incubation vessels could not be directly determined. The average weight of four measurements of the same pipetted volume, determined in a 100 mL beaker glass, was used.
Since the test substance was weighed directly into the incubation vessel and was considered unlikely to be air-stripped during incubation, no specific analyses were performed. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Unadapted secondary activated sludge was obtained (21-10-2014) from the WWTP Nieuwgraaf in Duiven. The WWTP Duiven is an activated sludge plant treating predominantly domestic wastewater.
Prior to use the activated sludge was homogenized with a syringe. The suspended solids (dry weight) content of the homogenized activated sludge was determined and subsequently concentrated by settlement to the required dry weight concentration. The activated sludge was aerated and fed overnight with OECD synthetic sewage concentrate at 50 mL/L prior to use on the day following collection. The suspended solids concentration of the activated sludge in the incubation vessels was 1.5 g dry weight/L. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20.1 to 20.7°C.
- pH:
- 6.9 to 7.1
- Nominal and measured concentrations:
- nominal concentrations: 18.0, 36.0, 70.0, 144.0, 284.0, 564.0 and 1148.0 mg/L
- Details on test conditions:
- Test conditions
The test was performed in 300 mL or 1 L Erlenmeyer flasks with total working volumes of 50 and 200 mL, respectively. The homogenized activated sludge was incubated in a shaking water bath (100 rpm, 20°C) for 3 hours with various concentrations of the test compound and synthetic sewage.
Synthetic sewage feed and stocks
The synthetic sewage feed was made by dissolving the following amounts of substances in 1 liter of deionized water: 16 g peptone, 11 g meat extract, 3 g urea, 0.7 g NaCl, 0.4 g CaCl2.2H2O, 0.2 g MgSO4.7H2O, 2.8 g K2HPO4. In the incubation vessels 1.6 or 6.4 mL of the synthetic sewage feed is added in a total volume of 50 and 200 mL, respectively. O,O-tert-butyl isopropyl monoperoxycarbonate was weighed directly in the incubation vessels. 3,5-Dichlorophenol was added to the incubation vessels using a stock solution of 1.0 g/L. The stock solution of 3,5-dichlorophenol was prepared by dissolving 0.1003 g of 3,5-dichlorophenol in approximately 40 mL of deionized water with the addition of a few drops of 1 M NaOH. Finally the pH was corrected with 1M H2SO4 to approximately pH 7 and the solution was diluted further to 100 mL with deionized water.
Test procedures
The activated sludge respiration inhibition test was performed according to the study plan. The study plan was developed from an OECD Test Guideline (OECD 209, 2010). In this test the inhibition of oxygen uptake by micro-organisms oxidizing organic carbon was not separately expressed from the respiration by micro-organisms oxidizing ammonium. The total respiration rate of activated sludge fed with a standard amount of synthetic sewage was measured. The respiration rate of the same activated sludge in the presence of various concentrations of the test substance under otherwise identical conditions was also measured. The inhibitory effect of the test substance at a particular concentration was expressed as a percentage of the mean respiration rates of the controls. Some aspects of the test are listed below:
• Prior to use the activated sludge was homogenized with a syringe.
• The activated sludge with test substances was incubated in a shaking bath (100 rpm) for 3 hours.
• The test substance and the reference compound (3,5-dichlorophenol) concentrations were spaced by a factor two. The tested nominal concentrations of the reference substance were 1.25, 2.5, 5, 10, 20 and 40 mg/L and 18.0, 36.0, 70.0, 144.0, 284.0, 564.0 and 1148.0 mg/L for the test substance.
An abiotic control was not performed because neither reduction of oxygen by the test substance nor formation of oxygen due to chemical breakdown of the test substance is expected. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 530 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Activated sludge respiration inhibition test
The inhibitory effect of O,O-tert-butyl isopropyl monoperoxycarbonate at a particular nominal concentration is expressed as a percentage of the two controls. From these results EC values were calculated. Computation of the EC confidence limits, on the basis of Fieller's theorem (Zerbe,1987), was not possible for the obtained dose response curve. The EC50 of O,O-tert-butyl isopropyl monoperoxycarbonate for activated sludge after 3 hours contact time is 530 mg/L. The EC10, EC20 and EC80 are 18, 58 and 4861 mg/L, respectively.
Quality criteria
The validity of the test is demonstrated by three validity criteria which were met according the guideline. First, the average oxygen uptake rate for the control replicates was 20.2 mg O2/g dry weight/hour, which is just meeting the criterion of >20 mg O2/g dry weight/hour. The inoculum used was obtained from a treatment plant which is operated at a low organic sludge load and as a consequence has a low oxygen uptake rate. Operating at low organic sludge loads is nowadays very common. A lower oxygen uptake rate does not influence the sensitivity of the activated sludge as shown by comparable EC50 values of the reference substance 3,5-dichlorophenol at oxygen uptake rates lower and higher than the prescribed rate (AkzoNobel, 2012). A lower average oxygen uptake rate does therefore not compromise the test outcome in terms of robustness of the endpoints obtained for the test and reference substance and does not invalidate the test. Second, the coefficient of variation of the replicates of the control oxygen uptake rates was 4%, which shows a high precision and fulfills the maximum prescribed variation of <30%. - Results with reference substance (positive control):
- The EC50 of the reference compound for activated sludge after 3 hours contact time is 13 mg/L, which is within the prescribed range of 2 to 25 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 of O,O-tert-butyl isopropyl monoperoxycarbonate for activated sludge after 3 hours contact time is 530 mg/L. The EC10, EC20 and EC80 are 18, 58 and 4861 mg/L, respectively.
- Executive summary:
In order to predict effects of chemicals in the environment and in biological waste water treatment plants, an activated sludge respiration inhibition test was performed. The toxicity to activated sludge was determined in accordance with OECD Test Guideline 209, and in compliance with the OECD principles of Good Laboratory Practice.
The test is valid as shown by the EC50 of the reference substance, 3,5-dichlorophenol (13 mg/L) and the coefficient of variation of the replicates of the control oxygen uptake rates (4%). The average oxygen uptake rate for the control replicates is 20.2 mg O2/g dry weight/hour this is just above the prescribed rate (>20 mg O2/g dry weight /hour). The toxicity of O,O-tert-butyl isopropyl monoperoxycarbonate to activated sludge was determined at a contact time of 3 hours, using various concentrations of the test substance. The inhibitory effect of O,O-tert-butyl isopropyl
monoperoxycarbonate at a particular nominal concentration is expressed as a percentage of the two controls. The EC50 of O,O-tert-butyl isopropyl monoperoxycarbonate for activated sludge after 3 hours contact time is 530 mg/L. The EC10, EC20 and EC80 are 18, 58 and 4861 mg/L, respectively.
Reference
Respiration rates of the activated sludge, inhibition percentages, pH and temperature at various concentrations of O,O-tert-butyl isopropyl monoperoxycarbonate after 3 hours contact time
Weighed test substance (mg) |
Concentration (mg/L) |
Activity (mg O2/g/min) |
Inhibition (%) |
pH at start test |
pH at end test |
Temperature at end test (°C) |
- |
Control |
0.3429 |
- |
7.0 |
6.9 |
20.7 |
- |
Control |
0.3381 |
- |
6.9 |
7.0 |
20.5 |
3.6# (0.00) |
18.0 |
0.2917 |
14 |
6.9 |
7.0 |
20.5 |
7.2# (0.05) |
36.0 |
0.3000 |
12 |
6.9 |
7.0 |
20.3 |
3.5 |
70.0 |
0.2667 |
22 |
6.9 |
6.9 |
20.1 |
7.2 |
144.0 |
0.2417 |
29 |
7.0 |
7.1 |
20.4 |
14.2 |
284.0 |
0.2286 |
33 |
7.0 |
7.1 |
20.4 |
28.2 |
564.0 |
0.1852 |
46 |
7.0 |
7.1 |
20.1 |
57.4 |
1148.0 |
0.0917 |
73 |
7.0 |
7.1 |
20.4 |
# = Test substance pipetted in 1 L incubation vessels, average weighed and between brackets the standard deviation of four measurements of a pipetted volume of the test substance.
Respiration rates of the activated sludge, inhibition percentages, pH and temperature at various concentrations of 3,5-dichlorophenol after 3 hours contact time
Concentration (mg/L) |
Activity (mg O2/g/min) |
Inhibition (%) |
pH at start test |
pH at end test |
Temperature at end test (°C) |
Control |
0.3167 |
- |
7.0 |
7.1 |
20.3 |
Control |
0.3286 |
- |
7.0 |
7.0 |
20.4 |
Control* |
0.3381 |
- |
6.9 |
7.0 |
20.5 |
Control* |
0.3524 |
- |
7.0 |
7.1 |
20.3 |
1.25* |
0.3083 |
11 |
7.0 |
7.0 |
20.3 |
2.5 |
0.2952 |
8 |
7.0 |
7.1 |
20.5 |
5.0 |
0.2875 |
11 |
7.1 |
7.0 |
20.2 |
10.0 |
0.2630 |
18 |
7.1 |
7.0 |
20.1 |
20.0 |
0.0815 |
75 |
7.1 |
7.0 |
20.4 |
40.0 |
0.0407 |
87 |
7.1 |
7.0 |
20.5 |
Concentration stock solution 3,5-dichlorophenol = 1000 mg/L pH stock solution 3,5-dichlorophenol = 7.7
* = corresponding control and reference substance concentration
Description of key information
Toxicity to microorganisms was determined in an activated sludge respiration inhibition test was performed according to OECD 209 under GLP.
The EC50 of O,O-tert-butyl isopropyl monoperoxycarbonate for activated sludge after 3 hours contact time is 530 mg/L. The EC10, EC20 and EC80 are 18, 58 and 4861 mg/L, respectively.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 530 mg/L
- EC10 or NOEC for microorganisms:
- 18 mg/L
Additional information
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