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EC number: 204-265-5 | CAS number: 118-61-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal sewage treatment plant, D-31137 Hildesheim.
- Storage at test facility: Refrigerator 7 +- 2°C, protected from light and moisture
- Pretreatment: The activated sludge was filtered with foided filter. The first 200 mL of the filtrate are not used. The second filtrate effluent from the domestic waste water is used to initiate inoculation.
- Concentration of sludge: 10^4 - 10^6 CFU/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 4 mg/L
- Based on:
- test mat.
- Remarks:
- corresponding to ThOD of 7.72 mg/L
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST ITEM:
- Dispersion treatement: Agitation
- Test concentration: 4.0 mg/L test material
- ThOD: 1.93 mg O2/mg test item
- ThOD in test vessel: 7.72 mgO2/L
CONTROL AND BLANK SYSTEM
- Inoculum blank: sodium acetate
- Test concentration: 10 mg/L
- ThOD: 0.78 mg O2/mg
INOCULUM CONTROL:
Nutrient solution and inoculum
TOXICITY CONTROL:
2.0 mg/L test item + 0.5 mg/L functional control item + nutrient solution and inoculum.
TEST CONDITIONS
- Duration: 28 days
- Frequency and duration of the application: Once per setting up over 28 d
- Test volume: 300 mL
- Composition of medium: Mineral nutrient solution according to OECD 301 D
PERFORMANCE OF THE TEST:
- One day before the test started the demineralized water for the test medium was aerated until oxygen saturation and then left at room temperature for at least 20 h.
- The test series was accompanied by a complete parallel series for the determination of the O2 demand of the inoculated control medium (C1, C2).
- All test solutions were made as stock solutions. 0.2 mL of the inoculum was given in each BOD bottle. The stock solution were filled to the BOD bottles with a siphon. The incubation vessels were closed without air bladders.
- At all measuring point the oxygen concentration of duplicates of the inoculum control, functional control, test item and toxicity control was determined.
TYPE AND FREQUENCY OF MEASUREMENTS:
- The temperature in the incubator was documented once every working day.
- At the beginning and the end of the test the pH value of the solution was determine
- The oxygen concentration was measured in the incubation vessels on day 0, 7, 14, 21 and 28.
- Test temperature: 20 °C
- pH measurement: yes
VALIDITY:
- The percentage degradation of the functional control must reach the pass level of 60% by day 14.
- The oxygen depletion in the inoculum control should not exceed 1.5 mg O2/L after 28 day.
- The residual concentrationof oxygen in the test bottles should not fall below 0.5 mg/L at any time.
- The differente of extremes of replicate values of removal of the test item at the end of the test, at the plateau or at the end of the 10-d-window as appropriate must be less than 20 %, if the test item is readily biodegradable.
- If in the toxicity test containing both test item and reference item, less than 25 % degradation occurred within 14 days, the test can be considered to be inhibitory.
TEST SYSTEM
- Culturing apparatus: Based on the ThOD of the test item the test concentration of 4.0 mg/L corresponding to a oxygen demand of 7.72 mgO2/L in the vessel was selected.
- Number of culture flasks/concentration: BOD bottles with glass stoppers (volume 300 mL)
- 10 bottles containing test item and inoculum (test suspension)
- 10 bottles containing only inoculum (inoculum control)
- 10 bottles containing reference item and inoculum (functional control)
- 10 bottles containing test and reference item and inoculum (toxicity control)
EVALUATION METHOD:
For the calculation of the BODn and the biodegradation of the test item, functional control and toxicity control the oxygen depletion of the control was taken into account. (please see detail under section "Any other information on materials and method" section) - Reference substance:
- acetic acid, sodium salt
- Remarks:
- sodium acetate
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 71
- Sampling time:
- 28 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 58
- Sampling time:
- 7 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 62
- Sampling time:
- 14 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 65
- Sampling time:
- 21 d
- Details on results:
- - Results of the toxicity control: 69% degradation after 14 days, indicating that the reference item is not inhibiting the activated sludge.
- Results inoculum control: oxygen demans came to 1.4 mg O2/L
- Results of functional control: pass level of > 60 % was reached after 6 days, fulfilling the validity criteria.
- Results with test item: pass level of 10 % was reached after 1 d and was 62% after 14 days - the 14-day window criteria was met. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item was found to be ready biodegradable, with a maximum degradation of 71 % after 28 days.
- Executive summary:
The study was performed according to OECD guideline 301 D and GLP principles, as a Closed Bottle Test was determined with a non adapted activated sludge for the test item. The test item concentration selected as appropriate was 4.0 mg/L, corresponding to a ThOD 7.72 mg O2/L in the test vessel.
The oxygen depletion in the inoculum control reached 1.40 mg O2/L until day 28. The residual concentration of oxygen in the test bottles did not fall below 0.5 mg/L at any time.
In order to check the activity of the study system sodium acetate was used as functional control. The functional control reached the pass level > 60% after 6 days. After 14 days a biodegradation rate of 69 % was reached.
In the toxicity control containing both test and reference item 69% degradation occured within 14 days. The degradation came to a maximum of 76% after 28 days. The degradation of the reference item was not inhibited by the test item.
The test item reached the 10% level after 1 day. The biodegradation came to 62 % after 14 days to a maximum of 71 % after 28 days.
According to CLP criteria, the test item must be regarded to be readily biodegradable
Reference
Results of the biodegradation trial, % degradation from 2 replicates
day | test item | toxicity control |
0 | - | 0 |
7 | 58 | 66 |
14 | 62 | 69 |
21 | 65 | 75 |
28 | 71 | 76 |
Description of key information
readily biodegradable (71% after 28 days; OECD 301 D)
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
The study was performed according to OECD guideline 301 D and GLP principles, as a Closed Bottle Test was determined with a non adapted activated sludge for the test item. The test item concentration selected as appropriate was 4.0 mg/L, corresponding to a ThOD 7.72 mg O2/L in the test vessel.
The oxygen depletion in the inoculum control reached 1.40 mg O2/L until day 28. The residual concentration of oxygen in the test bottles did not fall below 0.5 mg/L at any time.
In order to check the activity of the study system sodium acetate was used as functional control. The functional control reached the pass level > 60% after 6 days. After 14 days a biodegradation rate of 69 % was reached.
In the toxicity control containing both test and reference item 69% degradation occured within 14 days. The degradation came to a maximum of 76% after 28 days. The degradation of the reference item was not inhibited by the test item.
The test item reached the 10% level after 1 day. The biodegradation came to 62 % after 14 days to a maximum of 71 % after 28 days.
According to CLP criteria, the test item must be regarded to be readily biodegradable
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