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EC number: 203-937-5 | CAS number: 112-12-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from secondary source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- activated sludge, domestic
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2°C
- pH:
- 7.88 (at the start)
- Nominal and measured concentrations:
- Test chemical conc. used for the study were 0, 60, 120, 240, 480, 960 mg/l (nominal conc.), respectvely.
- Details on test conditions:
- TEST SYSTEM
- No. of vessels per concentration (replicates): 1 replicate
- No. of vessels per control (replicates): Duplicates - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol was used as a reference substance.
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 379.49 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- After 3 hours of contact time the inhibition of respiration by the test item was – 2 %, 22 %, 37%, 65 % and 68 % hours for the nominal test concentrations of 60, 120, 240, 480, 960 mg/L respectively.
Respiration rates for the two control vessels were comparable - Results with reference substance (positive control):
- The 3 hr EC50 value of the reference substance was determined to be 6.75 mg/l.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on the effect of test chemical on respiration inhibition of the test bacteria, the 3 hr EC50 value was determined to be 379.49 mg/l.
- Executive summary:
Toxicity to microorganisms study was carried out for 3 hrs. Study was performed in accordance with the OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test. Activated sludge, domestic was used as a test organism. Test inoculum were exposed to different nominal test chemical conc. (i.e., 0, 60, 120, 240, 480, 960 mg/l (nominal conc.)) for a period of 3 hrs. Test trestment was performed in 1 replicate and control system was performed in duplicates. Test conditions involve a temperature of 20 ± 2°C and a pH of 7.88 (at the start), respectively. 3,5-dichlorophenol was used as a reference substance. The 3 hr EC50 value of the reference substance was determined to be 6.75 mg/l. After 3 hours of contact time the inhibition of respiration by the test item was – 2 %, 22 %, 37%, 65 % and 68 % hours for the nominal test concentrations of 60, 120, 240, 480, 960 mg/L respectively. Respiration rates for the two control vessels were comparable. Based on the effect of test chemical on respiration inhibition of the test bacteria, the 3 hr EC50 value was determined to be 379.49 mg/l.
Reference
Description of key information
Toxicity to microorganisms study was carried out for 3 hrs (Secondary source, 2008). Study was performed in accordance with the OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test. Activated sludge, domestic was used as a test organism. Test inoculum were exposed to different nominal test chemical conc. (i.e., 0, 60, 120, 240, 480, 960 mg/l (nominal conc.)) for a period of 3 hrs. Test trestment was performed in 1 replicate and control system was performed in duplicates. Test conditions involve a temperature of 20 ± 2°C and a pH of 7.88 (at the start), respectively. 3,5-dichlorophenol was used as a reference substance. The 3 hr EC50 value of the reference substance was determined to be 6.75 mg/l. After 3 hours of contact time the inhibition of respiration by the test item was – 2 %, 22 %, 37%, 65 % and 68 % hours for the nominal test concentrations of 60, 120, 240, 480, 960 mg/L respectively. Respiration rates for the two control vessels were comparable. Based on the effect of test chemical on respiration inhibition of the test bacteria, the 3 hr EC50 value was determined to be 379.49 mg/l.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 379.49 mg/L
Additional information
Various experimental studies of the test chemical were reviewed for toxicity to microorganisms end point which are summarized as below:
In an experimental study from secondary source, toxicity to microorganisms study was carried out for 3 hrs. Study was performed in accordance with the OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test. Activated sludge, domestic was used as a test organism. Test inoculum were exposed to different nominal test chemical conc. (i.e., 0, 60, 120, 240, 480, 960 mg/l (nominal conc.)) for a period of 3 hrs. Test trestment was performed in 1 replicate and control system was performed in duplicates. Test conditions involve a temperature of 20 ± 2°C and a pH of 7.88 (at the start), respectively. 3,5-dichlorophenol was used as a reference substance. The 3 hr EC50 value of the reference substance was determined to be 6.75 mg/l. After 3 hours of contact time the inhibition of respiration by the test item was – 2 %, 22 %, 37%, 65 % and 68 % hours for the nominal test concentrations of 60, 120, 240, 480, 960 mg/L respectively. Respiration rates for the two control vessels were comparable. Based on the effect of test chemical on respiration inhibition of the test bacteria, the 3 hr EC50 value was determined to be 379.49 mg/l.
Another toxicity to microorganisms study was carried out. Tetrahymena pyriformis (ciliate) was used as a test bacteria. Stock solutions of each ketone were prepared in dimethyl sulfoxide (DMSO) at concentrations of 2.5, 5, 10, 25, or 50 parts per million. In all cases, the volume of stock solution added to each test flask did not exceed 0.35 ml, an amount that does not alter Tetrahymena population growth. Chemical was tested in a range-finder prior to testing in duplicate for three additional replicates. Only replicates with control-absorbency values from 0.6 to 0.9 were used in the analyses. Test bacteria were exposed to different test chemical conc. for a period of 2 days. All experiments were performed in duplicates. Population density was measured spectrophotometrically at 540 nm. The 50 percent growth inhibitory concentration, IGC50, was determined for each ketone using Probit Analysis of Statistical Analysis System (SAS) softwareI6 with Y as the absorbency normalized as percentage of control and X as the toxicant concentration in parts per million. Based on the effect on growth inhibition of the test bacteria, the 2 d IC50 value was determined to be 3652.8 mg/l (IC50 = 21.45 mM).
In a supporting study from peer reviewed journal and REAXY’s database,toxicity to microorganisms study was carried out. Vibrio fisheri was used as a test bacteria. It was bacteria were cultured to late log-phase growth and harvested. Bacteria was being frozen and maintained at -70°C in solution containing 15% dimethyl sulfoxide (DMSO). Test bacteria was thawed prior to testing and prepared in a 1:10 dilution in 2% NaCl. Stock solutions of each chemical were prepared in DMSO. Two-to-one serial dilutions of test chemical was made using 2% saline solution. Light emission was monitored using a Model 500 Analyzer (Azur Corp., Carlsbad, CA). All experiments were performed in duplicates. The 15-min EC50 values were determined using the Microtox Data Capture and Reporting Program, Version 7.82 (Azur Corp.). On the basis of the effect on growth inhibition of the test bacteria, the 15 mins EC50 value was determined to be 199.52 mg/l and the logarithm of the inverse of 15-min toxicity (pT15) was evaluated to be 2.3, respectively.
Additional toxicity to microorganisms study was carried out (Mark T. D. Cronin et. al., 1998). Tetrahymena pyriformis (ciliate) was used as a test bacteria. Study was performed under static conditions for 40 hrs. Population density of test organism Tetrahymena pyriformis was quantified spectrophotometrically. The reported endpoint is the 50% growth inhibitory concentration, IGC50. On the basis of the effect of test chemical on growth inhibition of the test bacteria, the 40 hr IC50 value was determined to be 31.62 mg/l (log(1/IG50) = 1.5).
For the test chemical, toxicity to microorganisms study was carried out. Tetrahymena pyriformis (ciliate) was used as a test bacteria. Study was performed under static conditions for 40 hrs. On the basis of the effect of test chemical on growth inhibition of the test bacteria, the 40 hr EC50 value was determined to be 5.76 mg/l.
On the basis of the above results, EC50 value of the test chemical was evaluated to be 5.76 to 3652.8 mg/l, respectively.
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