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EC number: 219-842-7 | CAS number: 2550-02-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to the justification for grouping of substances provided in IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 21.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.15 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Conclusions:
- A 48-h EC50 of 21.2 mg/L and a NOEC of 2.15 mg/L have been determined for the effects of the test substance on mobility of Daphnia magna.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002-04-03 to 2002-07-10
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- Test media were not prepared in accordance with OECD guidance for testing difficult substances
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Nominal treatments: 2.15, 4.64, 10.0, 21.5, 46.4 and 100 mg/L
- Sampling method: A sample of test media were taken from all treatments at the start (before introducing the test organisms) and at the end of the test. The sample taken at 48 hours was a combined aliquot from all 4 replicate vessels.
- Sample storage conditions before analysis: Not reported - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The highest test concentration was prepared by vigorously mixing 100 mg/l of the test substance with dilution water for 30 minutes. The other treatments were prepared by dilution of this solution.
- Controls: Dilution water - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain: IRCHA
- Source: Laboratory Culture
- Age at study initiation (mean and range, SD): 6-24 hours
ACCLIMATION
- Acclimation period: at least one week
- Acclimation conditions: same as test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 250 mg/l as CaCO3
- Test temperature:
- 19-21ºC
- pH:
- 7.66-8.54
- Dissolved oxygen:
- ≥80% ASV
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal loading rates: 0(Control), 2.15, 4.64, 10.0, 21.5, 46.4 and 100 mg/L.
Measured dissolved organic carbon concentrations were within 20% of the theoretical values at the end of the test.
The test results are interpreted with reference to nominal test concentrations - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size: glass, 38 mm diameter, 60 mm tall, 50 ml volume
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: aerated reconstituted fresh water
- Alkalinity: 0.8 mmol/l
- Ca/mg ratio: 4:1
- Culture medium different from test medium: no
- Intervals of water quality measurement: Start and end ot test
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light/8 h dark
- Light intensity: 500 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mobility after 24 and 48 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2 - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 21.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.15 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Immobilization of control: 2.5%
- Reported statistics and error estimates:
- EC50 values were determined by linear regression employing probit analysis. The NOEC value was determined directly from the raw data.
- Conclusions:
- A 48-h EC50 of 21.2 mg/L and a NOEC of 2.15 mg/L have been determined for the effects of the test substance on mobility of Daphnia magna.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1993-05-25 to 1993-05-27
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- The concentration of Dissolved Organic Carbon was determined in the stock solution used to prepare the test media. The stock solution concentration (203.5 mg/L DOC, equivalent to a test substance concentration of 454 mg/L) was used as the basis for determining the concentrations in the test media.
- Vehicle:
- no
- Details on test solutions:
- - Method: Test media prepared by dilution of a nominal 1000 mg/l stock solution prepared by mixing for 18 h followed by filtration. DOC concentration of stock solution determined and used as the basis for detting the other test concentrations.
- Treatments: Control and nominal test concentrations of 50,73, 100, 145, 204, 291 and 409 mg/L. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Clone 5
- Source: Stock culture
- Age at study initiation (mean and range, SD): <24 h
- Method of breeding: Parthenogenetic reproduction
- Feeding during test: None
ACCLIMATION
- Acclimation conditions (same as test or not): yes
- Type and amount of food: algae and a small quantity of sewage sludge. Sufficient to be consumed each day leaving no residue
- Feeding frequency: daily - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- none
- Hardness:
- The sum of the Ca2+ and Mg2+ ions was 2.5 mmol
- Test temperature:
- 20 +/-1ºC
- pH:
- 7.3 - 7.5
- Dissolved oxygen:
- 3.8 - 8.1 mg/L (the value of 3.8 mg/L was measured in the highest treatment level at the end of the test. All the daphnia were dead in this treatment)
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Control and nominal test concentrations of 50, 73, 100, 145, 204, 291 and 409 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: glass cylinder containing 10 ml of test medium
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 medium
- Intervals of water quality measurement: end of test
OTHER TEST CONDITIONS
- Photoperiod: Dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobilization after 24 and 48 h exposure
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.4-1.5
STATISTICS
EC50 and confidence interval determined by Probit analysis - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 177.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 148.9 - 199.1
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 140 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: 0
- Results with reference substance (positive control):
- - EC50: >0.9, <1.9 mg/L
- Reported statistics and error estimates:
- The EC50 values and 95% confidence intervals were calculated by Probit analysis. The NOEC was estimated directly from the raw data.
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 48-h EC50 value of 140 mg/L and NOEC of 100 mg/L have been determined for the effects of the test substance on mobility of Daphnia magna.
Referenceopen allclose all
Table 1. Test results
Nominal test substance loading rate (mg/L) | Mean percentage immobilisation after 24 hours | Mean percentage immobilisation after 48 hours |
0 (Control) | 0 | 2.5 |
2.15 | 0 | 0 |
4.64 | 0 | 10 |
10.0 | 10 | 20 |
21.5 | 0 | 15 |
46.4 | 15 | 30 |
100 | 90 | 100 |
Table 2. Results of analysis of test media
Nominal test substance loading rate (mg/L) | Theoretical DOC concentration (mg/l) | Actual DOC concentration at end of test (mg/l) |
0 (Control) | - | - |
2.15 | 1.0 | 0.9 |
4.63 | 2.1 | 0.6 |
10.0 | 4.5 | 5.2 |
21.5 | 9.7 | 8.7 |
46.3 | 20.8 | 16 |
100 | 44.9 | 43 |
Table 1. Test results
Nominal test substance concentration (mg/L) | Percentage immobile Daphnia after 24 hours | Percentage immobile Daphnia after 48 hours |
0 (Control) | 0 | 0 |
50 | 0 | 0 |
73 | 0 | 0 |
100 | 0 | 0 |
145 | 30 | 55 |
204 | 80 | 90 |
291 | 90 | 100 |
409 | 100 | 100 |
Description of key information
A 48-h EC50 of 21.2 -140 mg/l, Daphnia magna (OECD 202, read across from CAS 5089-70-3)
Key value for chemical safety assessment
Additional information
Note: An acute fish study, a daphnia test and a biodegradation screening study are currently ongoing for the registered substance (i.e. triethoxy(propyl)silane (CAS 2550-02-9)) in order to support the read across from (3-chloropropyl) triethoxysilane (CAS 5089-70-3) . This dossier will be updated once the final study reports are available.
Measured data were not available for triethoxypropylsilane. It was considered appropriate to read across from the structurally analagous substance, (3-chloropropyl) triethoxysilane (5089-70-3).
A 48-hour LC50value of 21.5 mg/l has been determined for the effects of the test substance on mobility of Daphnia magna, based on a guideline study (OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). The study is considered reliable and was selected as key.
In a second reliable study with (3 -chloropropyl) triethoxysilane (5089-70-3) higher values were obtained: A 48-h EC50value of 140 mg/l and NOEC of 100 mg/l for the effects of the test substance on Daphnia magna. This study was conducted according to EU Method C.2 (Acute Toxicity for Daphnia).
Additional good quality data for the analogue substance, trimethoxypropylsilane, have been read across for triethoxypropylsilane. Both substances share the same silanol hydrolysis product, propylsilanetriol; the other hydrolysis products being ethanol and methanol. A 48-h EC50 value of >816 mg/l and NOEC of ≥816 mg/l (EU Method C.2 (Acute Toxicity for Daphnia) have been determined for the effects of the test substance on mobility of Daphnia magna based on nominal concentrations of the substance.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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