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EC number: 953-797-0 | CAS number: 210300-09-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- Version / remarks:
- 2000
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3-hydroxy-4-methoxypyridine-2-carboxylic acid
- Cas Number:
- 210300-09-7
- Molecular formula:
- C7H7NO4
- IUPAC Name:
- 3-hydroxy-4-methoxypyridine-2-carboxylic acid
Constituent 1
- Specific details on test material used for the study:
- Substance name: X696476
Lot #: XS9-109113-80 (TSN300901)
Purity: 100%
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- yes
- Remarks:
- Deionised water
Test organisms
- Test organisms (inoculum):
- soil
Study design
- Total exposure duration:
- 28 d
Test conditions
- Test temperature:
- 20 to 22°C
- Moisture:
- Soil moisture: 47 to 50% of its maximum water holding capacity (MWHC)
- Nominal and measured concentrations:
- Low Dose: 0.107 mg/kg soil dry weight (corresponding to the PEC for 2 applications)
High Dose: 0.533 mg/kg soil dry weight (corresponding to 10x the single application PEC) - Reference substance (positive control):
- yes
- Remarks:
- The inhibition of nitrogen transformation by sodium chloride at a concentration of 16 g/kg soil dry weight will be determined at least once a year
Results and discussion
Effect concentrations
- Key result
- Duration:
- 28 d
- Remarks on result:
- other: test substance had no impact on soil nitrate content and soil nitrate formation rate of soil microflora when applied up to 0.533 mg/kg soil dry weight. It can be concluded that the test substance will not have any long term influence on soil microorganism
- Results with reference substance (positive control):
- The reference item sodium chloride has a significant impact on nitrogen turnover and respiration activities of soil microflora when applied at 16 g/kg soil dry weight. The reference item sodium chloride has long term influence on soil microflora and confirms the sensitivity of the test system (assessed in a previous study).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the results of this study, it is concluded that the test substance had no impact on soil nitrate content and soil nitrate formation rate of soil microflora when applied up to 0.533 mg/kg soil dry weight. It can be concluded that the test substance will not have any long term influence on soil microorganisms.
- Executive summary:
The effects of the test substance on soil microflora were assessed in accordance with the OECD Guidelines 216.
Determination of nitrogen-transformation (ammonium-, nitrite and nitrate-nitrogen levels) in soil enriched with lucerne meal (concentration in soil 0.5%). Comparison of test item treated soil with a non-treated soil. Three replicates per treatment and concentration. NH4-, NO2- and NO3-nitrogen formed from the nitrification process was determined by means of a Continuous Flow Analyzer system (AA3, XY-2 / XY-3 Sampler).
Sampling scheme: 0, 7, 14 and 28 days after treatment; Subsamples were withdrawn from the soil bulk batches and subjected to analysis.
Soil Nitrate Content: No adverse effects of the test substance on nitrogen transformation in soil could be observed in both test item concentrations (0.107 mg/kg dry soil and 0.533 mg/kg dry soil) after 28 days. Only slight and not significant deviations from the control of 1.06% (lower rate) and 1.24% (higher rate) were measured at the end of the 28-day incubation period.
Nitrate Formation Rate: The soil nitrate formation rates were calculated on an incremental basis (i.e. between successive sampling dates). In the last interval between days 14 and 28, the deviations from control were -3.85% and 1.92% for the lower and higher test concentration of the test substance.
Thus, the difference in the soil nitrate formation rates between the control and both test item treatments was clearly below the OECD guideline 216 trigger value of 25% at the 14 to 28 day interval. These deviations were not statistically significant different (Student t-test, α = 0.05).
Validity Criteria: The variation between the replicate control samples was within the validity criterion of 15% for the nitrogen transformation test (OECD test guidelines 216) throughout the test. The validity of the test system was further confirmed by the sensitivity established in separate positive control experiments using sodium chloride at a concentration of 16 g/kg soil dry weight.
Based on the results of this study, it is concluded that the test substance had no impact on soil nitrate content and soil nitrate formation rate of soil microflora when applied up to 0.533 mg/kg soil dry weight. It can be concluded that the test substance will not have any long term influence on soil microorganisms.
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