3- Hydroxy-4-methyoxypyrdine-2-carboxylic acid

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 (Bioaccumulation in Fish: Aqueous and Dietary Exposure) -I: Aqueous Exposure Bioconcentration Fish Test

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-hydroxy-4-methoxypyridine-2-carboxylic acid

Cas Number:

210300-09-7

Molecular formula:

C7H7NO4

IUPAC Name:

3-hydroxy-4-methoxypyridine-2-carboxylic acid

Specific details on test material used for the study:

Substance ID: X696476
Lot #: YH1-143590-18-3
Purity: 100%

Radiolabeled substance
Substance ID: 14C-X12335145
Lot #: YH2-144026-008-MW
Radiochemical purity: 100%
Specific activity: 30.0 µCi/mmol

Radiolabelling:

yes

Test solutions

Vehicle:

yes

Remarks:

Dilution water

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Study design

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

other: laboratory freshwater

Total exposure / uptake duration:

14 d

Total depuration duration:

14 d

Test conditions

Hardness:

140 to 154 mg CaCO3/L

Test temperature:

22.7 to 23.5°C

pH:

8.32 to 8.46

Dissolved oxygen:

7.81 to 8.51 mg/L

Conductivity:

331 to 342 μS/cm

Nominal and measured concentrations:

Nominal Concentrations: 0 (control) and 0.10 mg a.i./L

Results and discussion

Lipid content

Lipid content 1

Lipid content:

10.1 %

Time point:

other: overall

Lipid content 2

Lipid content:

9.53 %

Time point:

end of exposure

Bioaccumulation factor

Bioaccumulation factor 1

Key result

Conc. / dose:

0.102 mg/L

Temp.:

>= 22.7 - <= 23.5 °C

pH:

8.32

Type:

BCF

Value:

1.8 L/kg

Basis:

other: whole fish

Calculation basis:

other: steady state and kinetic

Bioaccumulation factor 2

Key result

Conc. / dose:

0.102 mg/L

Temp.:

>= 22.7 - <= 23.5 °C

pH:

8.32

Type:

BCF

Value:

0.89 L/kg

Basis:

other: whole fish

Calculation basis:

other: 5% Lipid corrected, Steady State and Kinetic

Depuration

Key result

Parameter:

other: Time to 95% Depuration

Depuration time (DT):

25 d

Rate constants

Rate constants 1

Rate constant:

other: uptake rate constant (L/kg/day)

Value:

0.214

Rate constants 2

Rate constant:

other: depuration rate constant (day-1)

Value:

0.12

Applicant's summary and conclusion

Conclusions:

Time to Steady State: 14 days
Time to 95% Depuration: 25 days
Steady State BCFss: 1.8 L/kg
TRR Kinetic BCFk: 1.8 L/kg
5% Lipid corrected, Steady State BCFssl: 0.89 L/kg
5% Lipid corrected, Kinetic BCFkl: 0.89 L/kg

Executive summary:

The bioconcentration potential of the test substance was evaluated by zebrafish, Danio rerio, exposed to one concentration of carbon-14 labeled test substance based on the OECD Guideline 305, Bioconcentration in Fish: Aqueous and Dietary Exposure.

Zebrafish, Danio rerio, were exposed to the test substance under flow-through conditions at one mean measured concentration, 0.102 mg TRR/L.

Steady state equilibrium tissue residues in whole fish were determined to be approximately 0.18 mg/kg, yielding an approximate steady state BCFss of 1.8 L/kg. Based on the ratio of the uptake and depuration rate constants (k1 = 0.2140 L/kg/day, and k2 = 0.1198 per day, respectively) the whole fish BCFk was calculated to be 1.8. Correction of the BCF values to a 5% lipid content yielded estimates of the BCFssl = 0.89 L/kg and the BCFkl = 0.89 L/kg.

Following transfer to clean water the whole body 14C-residue was rapidly depurated, with the calculated time to 95% depuration of the 14C-residue being 25 days. Radioactive residues extracted from the fish tissues did not match the retention time of the parent, indicating that the extracted residues were not the parent compound.