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EC number: 209-533-5 | CAS number: 584-13-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06.09.2021 to 10.09.2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 021
- Report date:
- 2021
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- Adopted: April 13, 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- report annex: Test Facility GLP Certificate
Test material
- Reference substance name:
- 4H-1,2,4-triazol-4-ylamine
- EC Number:
- 209-533-5
- EC Name:
- 4H-1,2,4-triazol-4-ylamine
- Cas Number:
- 584-13-4
- Molecular formula:
- C2H4N4
- IUPAC Name:
- 4H-1,2,4-triazol-4-ylamine
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: the highest (100 mg·L-1) and the lowest test concentration (1.0 mg·L-1)
- Sampling method: The samples for analysis (0 hours) were prepared at the beginning of the test and immediately delivered in transport box to analytical laboratory. The samples for analysis at the end of the test (48 hours) were delivered to analytical laboratory immediately after the end of testing. The samples were analysed on the day of delivery.
- Sample storage conditions before analysis: All samples were stored at laboratory temperature.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
The stock solution of the test item was prepared in the dilution water. 50 mg of the test item was weighed into 500 mL of the dilution water for the preliminary test. The concentrations
of solutions used in the preliminary test were obtained by dilution of the stock solution with dilution water. The exact concentrations are given on tables in Chapter 4.1. of study report.
The stock solution of the test item in dilution water was prepared for the preliminary test. The test was performed in a range of nominal concentrations from 1 mg·L-1 to 100 mg·L-1. The test solutions were prepared by dosing of appropriate volumes of the stock solution into volumetric flasks and replenishment up to mark with dilution water. Control test was performed concurrently with dilution water only. The test was performed in four replicates. The volume of the test solution was 50 mL in each test beaker. 5 animals were put into each beaker. Total number of daphnia used for each test concentration and control was 20. The daphnia immobilisation was observed and recorded after 24 and 48 hours. At the beginning and at the end of the test the oxygen content and pH values were measured. The ambient temperature was recorded throughout the test. The conductivity of deionized water was measured at the beginning of the test. The content of Ca + Mg were measured in dilution water at the beginning of the test.
Using the procedures described in EU method, a limit test may be performed at 100 mg·L-1 of test item in order to demonstrate that the EC50 is greater than this concentration. The limit test should be performed using 20 daphnids with the same number in the control. Because the preliminary test was performed according criteria of limit test, results are in line with EU method requirements, further testing is not needed and the study was terminated.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Strauss
- Source: own laboratory breeding
- Age at study initiation (mean and range, SD): young daphnia born in 24 hours
- Method of breeding: aperiodic parthenogenesis
- Feeding during test: no
ACCLIMATION
- Acclimation period: The grow-up gravid females are separated into dilution water. Young daphnia born in 24 hours are segregated to the handling container from which they entered the experiment.
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: a mixture of algae
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Content of (Ca+Mg) in dilution water: 2.5 mmol.L-1
- Test temperature:
- in general 20 +/- 2°C, with a variation of +/- 1°C at each individual test;
test range of temperature: 18.9 – 19.8 °C - pH:
- 8
- Dissolved oxygen:
- 8.0 mg.L-1
- Conductivity:
- 1.94 uS/cm
- Nominal and measured concentrations:
- Nominal concentrations: 100, 50, 10, 5, 1 mg·L-1
The analytical determination of the test substance concentrations was performed at the beginning and at the end of the test. The samples for analysis were taken from the highest (49 mg.L-1) and the lowest (10 mg.L-1) test concentration:
Measured concentration: 1.05 mg.L-1 and 100.3 mg.L-1 (0 hours), 1.02 mg.L-1 and 102.4 mg.L-1 (48 hours) - Details on test conditions:
- TEST SYSTEM
- Test vessel: open beakers
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: one daphnia per 50 mL of test solution (least 2 mL per individual)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Standard dilution water was prepared just before the test.
- The dilution water should be of pH 7.8 +/- 0.2. The sum of Ca + Mg ions should be approx. 2.5 mmol.L-1.
- Ca/mg ratio: 2.5 mmol.L-1
- Conductivity: 1.94 uS/cm
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark cycle
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The daphnia immobilisation was observed after 24 and 48 hours.
TEST CONCENTRATIONS
Range finding study
- Test concentrations: The test was performed in range nominal concentrations from 1 mg.L-1 to 100 mg.L-1.
Using the procedures described in EU method, a limit test may be performed at 100 mg·L-1 of test item in order to demonstrate that the EC50 is greater than this concentration. The limit test should be performed using 20 daphnids with the same number in the control. Because the preliminary test was performed according criteria of limit test, results are in line with EU method requirements, further testing is not needed and the study was terminated. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes. The immobilisation of test organism by the reference substance (48h – EC50) obtained from last reference test meets the calculated range from the interlaboratory test.
Reference test:
48 hour – EC50 = 0.73 mg.L-1 (95% confidence limit: 0.63 – 0.85 mg.L-1)
Interlaboratory test:
48 hour – EC50 = 0.50 – 1.16 mg.L-1
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The experiment with the nominal concentration of 100 mg·L-1 included in the preliminary test has been performed using 20 daphnia, therethrough the conditions of the limit test given
in guidelines have been fulfilled. It was demonstrated that the EC50 is higher than this concentration.
No immobilisation was observed in the test. Therefore exact values of EC50 could not be calculated and the values of EC are given in the form of a range. The EC0 and EC100 values are identified by direct observation.
24 hour – EC50 > 100 mg·L-1 (nominal concentration)
48 hour – EC50 > 100 mg·L-1 (nominal concentration)
24 hour – EC0 = 100 mg·L-1 (nominal concentration)
48 hour – EC0 = 100 mg·L-1 (nominal concentration)
24 hour – EC100 > 100 mg·L-1 (nominal concentration)
48 hour – EC100 > 100 mg·L-1 (nominal concentration) - Executive summary:
The test item, 4H-1,2,4-triazol-4-amine, was tested in acute immobilisation test on Daphnia magna.
The test was performed according to method C.2 – Daphnia sp. Acute Immobilisation Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.
The preliminary test was performed in a range of the test item nominal concentrations 1 – 100 mg·L-1. The test was performed as static.
Based on no toxicity of the test item found in the preliminary test, the study was terminated. The experiment with the concentration of 100 mg·L-1 included in the preliminary test has been performed using 20 daphnia, therethrough the conditions of the limit test given in guideline have been fulfilled.
It was demonstrated that the EC50 is higher than this concentration.
There is stated in the guideline that, if evidence is available to demonstrate that the concentration of the test item has been satisfactorily maintained within ± 20 percent of the nominal or measured initial concentration throughout the test, the results can be based on nominal or measured initial values.
In this study concentration of the test item has been satisfactorily maintained within ± 20 percent of the nominal concentration throughout the test.
Further the nominal concentrations were used for all evaluation and results.
Test results:
24 hour – EC50 > 100 mg·L-1 (nominal concentration)
48 hour – EC50 > 100 mg·L-1 (nominal concentration)
24 hour – EC0 = 100 mg·L-1 (nominal concentration)
48 hour – EC0 = 100 mg·L-1 (nominal concentration)
24 hour – EC100 > 100 mg·L-1 (nominal concentration)
48 hour – EC100 > 100 mg·L-1 (nominal concentration)
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