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EC number: 223-211-1 | CAS number: 3770-97-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018-04-13 to 2018-05-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 22 July 2010
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 30 May 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: As the test substance was known to be highly degradable in water, appropriate amounts of test substance were weighed and stored frozen overnight (-20ºC) until use. On the day of the study the test substance samples were added by direct addition to appropriate vessels immediately before the inoculum was added (to minimise degradation).
- Controls: pure water, synthetic sewage and inoculum, but without addition of test item - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Test System: Activated sludge
Origin: Burley Menston Sewage Treatment Works (West Yorkshire, U.K.)
Reason for the selection: Predominantly domestic catchment
Pretreatment: The sludge was transported to the test facility in a closed container with an adequate headspace to prevent the sample from becoming anoxic. On arrival at the test facility, the sludge was aerated with a compressed air supply standardly delivered through an air pump.
The suspended solids concentration was determined gravimetrically following homogenisation and adjusted using dechlorinated tap water to 3 g/L (± 0.3 g/L), as necessary. The suspended solids content was determined to be 2.8 g/L for the definitive test.
The sludge was maintained at 20 ± 2°C with aeration. The sludge was collected on the 12 June 2018 and stored for 1 day before use for the definitive test. When stored, the sludge was maintained at 20 ± 2°C and fed daily with synthetic sewage concentrate at a rate of 50 mL/L.
The initial pHs of the sludge for the definitive was 6.81. The sludge used was adjusted with NaOH to a pH 7.55. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 20.4°C
- pH:
- control: 7.15 to 7.19
test item: 7.11 to 7.54 - Dissolved oxygen:
- The dissolved oxygen level at the start of the measurement was low (ca 4 mg/L) and plummeted to just above 2 mg/L, before it levelled into a linear line, therefore, the rate has been calculated on a short linear line which extended below 2 mg/L.
- Nominal and measured concentrations:
- test item: 62.5, 125, 250, 500, 1000 mg/L (nominal)
positive control: 0.1, 2.0 and 40 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL glass conical flasks
- Type: open
- Aeration: yes
- No. of vessels per test item concentration (replicates): 3
- No. of vessels per positive control concentration (replicates): 1
- No. of vessels of control (replicates): 4
- Sludge concentration (weight of dry solids per volume): 3 g/L
- Nitrification inhibitor used: N-allylthiourea
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Test item concentrations: 62.5, 125, 250, 500, 1000 mg/L (nominal) - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- 3,5-Dichlorophenol
EC50 = 12 mg/L (total respiration) - Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal test concentrations, the NOEC for nitrification, total respiration and heterotrophic respiration was estimated to be 1000 mg/L.
- Executive summary:
The effects of the test substance, F6-chloride on the activated sludge micro-organisms of the biological (aerobic) stage of waste-water treatment plants was determined in accordance with OECD Guideline 209 (Adopted July 2010). Based on nominal test concentrations, the EC50 for total respiration, heterotrophic respiration and nitrification respiration were estimated to be >1000 mg/L respectively. Based on nominal test concentrations, the NOEC for nitrification, total respiration and heterotrophic respiration was estimated to be 1000 mg/L. All validity criteria were met therefore the test is considered valid.
Reference
Description of key information
The test item had no inhibiting effects on the respiration rate of activated sludge up to and including 1000 mg/L.
The NOEC was determined to be 1000 mg /L. The 3-hour EC50 value exceeded 1000 mg/ and could not be established.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
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