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EC number: 259-467-6 | CAS number: 55067-10-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- From 14th to 28th November 1991.
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- not specified
Test material
- Reference substance name:
- (1,4,5,6-tetrahydro-4,6-dioxopyrimidin-2-yl)cyanamide
- EC Number:
- 259-467-6
- EC Name:
- (1,4,5,6-tetrahydro-4,6-dioxopyrimidin-2-yl)cyanamide
- Cas Number:
- 55067-10-2
- Molecular formula:
- C5H4N4O2
- IUPAC Name:
- (1,4,5,6-tetrahydro-4,6-dioxopyrimidin-2-yl)cyanamide
- Test material form:
- solid: particulate/powder
- Details on test material:
- Test material: CYDI ((1,4,5,6-Tetrahydro-4,6-dioxopyrimidin-2-yl)cyanamide)
Description: Off-white powder
Storage: In original container at room temperature
Molecular formula: C5H4N4O2
Constituent 1
- Specific details on test material used for the study:
- Test material: CYDI ((1,4,5,6-tetrahydro-4,6-dioxopyrimidin-2-yl)cyanamide).
Description: Off-white powder.
Storage: In original container at room temperature.
Molecular formula: C5H4N4O2.
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Direct dispersion in water.
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- A mixed population of activated sewage sludge microorganisms.
Souce: The aeration Stage of Severn Trent PLC Sewage Treatment Plant, Belper, Derbyshire.
Study design
- Test type:
- not specified
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- Not specified.
- Test temperature:
- 21°C.
- pH:
- 6.5
- Dissolved oxygen:
- Not specified.
- Salinity:
- Not specified.
- Conductivity:
- Not specified.
- Nominal and measured concentrations:
- Nominal concentrations: 1, 10, 100 and 1000 mg/L in a range-finding study.
3 replicates of 100 mg/L in the final study. - Details on test conditions:
- The sample was maintained at 21 " C wi th continuous aeration and used on the day of collection. The pH was buffered to pH 6.5 with sodium hydroxide solution and the suspended solids level adiusted to 3.6 g/l prior to use.
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- EC50 (3-hour contact time) = 8.8 mg/L.
Any other information on results incl. tables
Table 1: Range-finding study
Concentration (mg/l) |
O2 consumption rates mgO2/l/min 3-hour contact time |
% Inhibition |
Control 1 |
0.52 |
- |
Control 2 |
0.50 |
- |
1.0 |
0.52 |
<2> |
10 |
0.51 |
0 |
100 |
0.50 |
2 |
1000 |
0.01 |
98 |
Reference 3.2 mg/L |
0.43 |
16 |
Reference 32 mg/L |
0.10 |
80 |
Table 2: 100 mg/L test substance replicates
Concentration (mg/1) |
O2 consumption rates mgO2/l/min 3-hour contact time |
% Inhibition |
Control 1 |
0.44 |
- |
Control 2 |
0.45 |
- |
100, Replicate 1 |
0.43 |
3 |
100, Replicate 2 |
0.44 |
1 |
100, Replicate 3 |
0.41 |
8 |
Reference 3.2 mg/L |
0.35 |
21 |
Reference 32 mg/L |
0.06 |
87 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The oxygen consumption rate of activated sludge decreases by an average of 4% in the presence of 100 mg/L of (1,4,5,6-tetrahydro-4,6-dioxopyrimidin-2-yl)cyanamide.
The EC50 after 3 hours of exposure was therefore determined to be > 100 mg/L. - Executive summary:
Respiration inhibition of (1,4,5,6-tetrahydro-4,6-dioxopyrimidin-2-yl)cyanamide on activated sludge has been studied. The activated sludge was subjected to various concentrations of test substance for 3 consecutive hours.
A preliminary study has been carried out with test substance concentrations of 1, 10, 100 and 1000 mg/L.
Based on the results obtained in this preliminary test, it was decided to make new measurements on 100 mg/L replicates.
The average respiration inhibition based on the 100 mg/L replicates was found to be 4%.
The EC50 for respiration inhibition of activated sludge by (1,4,5,6-tetrahydro-4,6-dioxopyrimidin-2-yl)cyanamide after 3 hours of exposure was therefore determined to be > 100 mg/L.
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