Dipotassium 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl)amino-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate

Endpoint summary

Description of key information

Short term toxicity to aquatic invertebrates:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on daphnia magna predicted for Dipotassium 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl) amino-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate (71230-67-6). The EC50 value was estimated to be 320.093 mg/l when Dipotassium 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl)amino-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonateexposed to daphnia magna for 48 hrs.

Toxicity to aquatic algae qna cyanobacteria:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) was predicted for Dipotassium 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl)amino-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate (71230-67-6). The EC50 value was estimated to be 102.640mg/l when Dipotassium 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl)amino-1,3,5-triazin-2-yl]amino]stilbene- 2,2'-disulphonate exposed to Desmodesmus subspicatus for 72hrs.

Additional information

Summarized results of toxic effect of the chemicalDipotassium 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl) amino-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate (71230-67-6)on the growth and other normal activities of aquatic lifes, by the collection of data from various databases and also predicting the effects of chemical on the basis of structure similarities.

Short term toxicity to aquatic invertebrates:

Toxicity of chemical Dipotassium 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl) amino-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate (71230-67-6) on the growth of aquatic life’s have been studied and concluded the nature and classification category on the basis of data obtain from various sources, RA chemicals and also from predicted QSAR reports which support the classification of the target chemical.

In the first predicted weight of evidence study forDipotassium 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl) amino-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate (71230-67-6). Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on daphnia magna predicted for Dipotassium 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl) amino-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate (71230-67-6). The EC50 value was estimated to be 320.093 mg/l when Dipotassium 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl)amino-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonateexposed to daphnia magna for 48 hrs.

Similarly in the second weight of evidence study for RA chemical Remazol black B; tetrasodium 4-amino-5-hydroxy-3,6-bis[(4-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)diazenyl]naphthalene (17095-24-8) J-check 2017, The short term toxicity to aquatic invertebrates was performed using OECD guideline 202. The experiment was conducted for 48 hrs and effective concentration was determined. After 48 hrs of experiment EC50 value for the short term toxicity to aquatic invertebrates was determined to be >1000 mg/l for Remazol black B. Based on the result, the Remazol black B was considered to be not toxic and can be consider to be not classified as per CLP regulations.

 

Similarly in the third weight of evidence study for RA chemical Disodium salt 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl)amino]-1,3,5-triazin-2-yl]amino]stilbene-2,2'- disulphonate(4193-55-9), SIDS report Determination of short term toxicity of Disodium salt 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl)amino]-1,3,5-triazin-2-yl]amino]stilbene-2,2'- disulphonate on the growth of daphnia magna. Test was performed in static system under the proper temperature and pH conditions as per the guidelines. No immobilization and abnormal behaviour of the Daphnia was observed in the blank control. At 100 mg/l, after 24 h one Daphnia was immobilized in one jar (5 % immobilisation). After 48 h one Daphnia was immobilized in each jar (10 % immobilisation). Therefore, the EC0 value after 24 and 48 h was below 100 mg/l. The EC50 value after 24 and 48 h could not be determined, but was clearly higher than 100 mg/l. Based on the immobilisation of daphnia magna due to the exposure of chemical Disodium salt 4,4'-bis[6-anilino-4-[bis(2hydroxyethyl) - amino] -1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate for 48hrs, the EC0 was <100 mg/l and the EC50 was > 100mg/l.

 

Similarly in the fourth weight of evidence study for RA chemicalC.I. Fluorescent brightening agent 28 (Benzenesulfonic acid, 2,2'-(1,2-ethenediyl)bis[5-[[4-[bis(2-hydroxyethyl) amino]-6-(phenylamino)-1,3,5-triazin-2-yl]amino]-) (4404-43-7) SIDS report, HPVIS 2017. Short term toxicity of C.I. Fluorescent brightening agent 28 (Benzenesulfonic acid, 2,2'-(1,2-ethenediyl)bis[5-[[4-[bis(2-hydroxyethyl) amino]-6-(phenylamino)-1,3,5-triazin-2-yl]amino]-) on the growth of daphnia magna. Study was performed for the 24hrs in the static system. Based on the immobilization of daphnia magna due to the exposure of C.I. Fluorescent brightening agent 28, the EC0 was >1000 mg/l.

Thus based on the data available for Dipotassium 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl) amino-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate (71230-67-6)from various databases, and QSAR toolbox version 3.3,Dipotassium 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl) amino-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate (71230-67-6)was consider to be nontoxic and thus can be consider to be not classified as per the CLP classification criteria.

Toxicity to aquatic algae qna cyanobacteria:

Toxicity of chemical Dipotassium 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl) amino-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate (71230-67-6)on the growth of aquatic life’s have been studied and concluded the nature and classification category on the basis of data obtain from various sources, RA chemicals and also from predicted QSAR reports which support the classification of the target chemical.

 

In the first predicted weight of evidence study forDipotassium 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl) amino-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate (71230-67-6). Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) was predicted for Dipotassium 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl)amino-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate (71230-67-6). The EC50 value was estimated to be 102.640mg/l when Dipotassium 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl)amino-1,3,5-triazin-2-yl]amino]stilbene- 2,2'-disulphonate exposed to Desmodesmus subspicatus for 72hrs.

Similarly in the second weight of evidence study for RA chemicalHydrogen [4-[4-(diethylamino)-2',4'- disulphonatobenzhydrylidene] cyclohexa- 2,5-dien-1-ylidene] diethylammonium, sodium salt, (CAS No.129-17-9), UERL report 2015. The effect of test item Hydrogen [4-[4-(diethylamino)-2',4'- disulphonatobenzhydrylidene] cyclohexa- 2,5-dien-1-ylidene] diethylammonium, sodium salt, (CAS No.129-17-9) was studied on the growth of fresh water green algaChlorella vulgaris.The study was conducted following OECD guideline 201- Alga growth inhibition test. The test concentration chosen for the study were 6.25 mg/l, 12.5 mg/l, 25 mg/l, 50 mg/l, 100 mg/l and 200 mg/l. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 123.386 mg/L. Thus, based on this value, it can be concluded that the test chemical can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.

 

 

Similarly in the third weight of evidence study for RA chemicalDisodium salt 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl)amino]-1,3,5-triazin-2-yl]amino]stilbene-2,2'- disulphonate(4193-55-9), HPVIS report, Evaluations of the effect of Disodium 4,4-bis[6-anilino-[4-[bis2-hydroxyethylamino]-1,3,5-triazin-2-yl]amino]stilbene-2,2-disulphonate on the growth of Pseudokirchnerella subcapitata. Test was performed according to the standard OECD guidelines in the static system for total 72hrs. After the 72hrs of exposure at the test concentration of 100 mg/l a slight inhibition of algae growth (18.4 % for biomass, 5.3 % for growth rate) was observed. Therefore, the NOEC and EC0 was determined to be below 100 mg/l, the EC50 was > 100 mg/l. Based on the inhibition of growth rate and the biomass of green algae Pseudokirchnerella subcapitata by the contact with chemical Disodium 4,4-bis[6-anilino-[4-[bis2-hydroxyethylamino]-1,3,5-triazin-2-yl]amino]stilbene-2,2-disulphonate for 72hrs, the EC50 was > 100 mg/l. Thus the chemical was consider to nontoxic and cannot be consider to be classified as toxic as per the CLP classification criteria.

 

Similarly in the fourth weight of evidence study for RA chemical Benzenesulfonic acid, 2,2'-(1,2-ethenediyl)bis[5-[[4-[bis(2-hydroxyethyl)amino]-6-[(4-sulfophenyl) amino]-1, 3,5 -triazin-2-yl]amino]-,tetrasodium salt (16470-24-9), HPVIS databases, 2017. Evaluations of the effect of Benzenesulfonic acid, 2,2'-(1,2-ethenediyl)bis[5-[[4-[bis(2-hydroxyethyl)amino]-6-[(4-sulfophenyl) amino]-1, 3,5 -triazin-2-yl]amino]-,tetrasodium salt on the growth of Scenedesmus subspicatus. Test was performed according to the standard OECD guidelines in the static system for total 72hrs. Based on the growth rate inhibition of Scenedesmus subspicatus by the chemical exposure Benzenesulfonic acid, 2,2'-(1,2-ethenediyl) bis[5-[[4-[bis(2-hydroxyethyl)amino]-6-[(4-sulfophenyl)amino]-1,3,5-triazin-2-yl]amino]-,tetrasodium salt for 96hrs, the EC50 was > 1000 mg/l, NOEC was 500mg/l and the LOEC was 1000 mg/l. Thus the chemical was consider to nontoxic and cannot be consider to be classified as toxic as per the CLP classification criteria.

 

Thus based on the data available for Dipotassium 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl) amino-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate (71230-67-6) from various databases, and QSAR toolbox version 3.3,Dipotassium 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl) amino-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate (71230-67-6) was consider to be nontoxic and thus can be consider to be not classified as per the CLP classification criteria.

Based on the overall data for target chemical by gathering data from various sources for evaluating the toxic nature of chemical on invertebrates and algae, it was concluded that dipotassium 4,4'-bis[6-anilino-4-[bis(2-hydroxyethyl) amino-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate (71230-67-6) was consider to be nontoxic and thus can be consider to be not classified as toxic as per the CLP classification criteria.