Benzoic acid, 3,3'-[(2,5-dimethyl-1,4-phenylene)bis[imino(1-acetyl-2-oxo-2,1-ethanediyl)azo]]bis[4-chloro-

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study without analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

yes

Remarks:

, instead a centrifuged sludge a settled sludge was used; due to the expected toxicity, only one concentration (100 mg/l) was tested in duplicate.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-[(2E)-2-[1-({4-[(2E)-2-[2-(5-carboxy-2-chlorophenyl)hydrazin-1-ylidene]-3-oxobutanamido]-2,5-dimethylphenyl}carbamoyl)-2-oxopropylidene]hydrazin-1-yl]-4-chlorobenzoic acid

EC Number:

616-083-7

Cas Number:

74351-78-3

Molecular formula:

C30 H26 Cl2 N6 O8

IUPAC Name:

3-[(2E)-2-[1-({4-[(2E)-2-[2-(5-carboxy-2-chlorophenyl)hydrazin-1-ylidene]-3-oxobutanamido]-2,5-dimethylphenyl}carbamoyl)-2-oxopropylidene]hydrazin-1-yl]-4-chlorobenzoic acid

Details on test material:

- Name of test material (as cited in study report): TK 10114/ZP
- Physical state: brown powder
- Analytical purity: commercial grade
- Lot/batch No.: OP 31
- Expiration date of the lot/batch: 1992-03
- Storage condition of test material: at room temperature

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls: yes

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Laboratory culture: Activated sludge collected from the sewage treatment plant of CH-4153 Reinach on the 26th of April in 1988
- Preparation of inoculum for exposure: The preparation was carried out according to the method described in the guideline. Except settling instead of centrifugation
- Initial biomass concentration: sludge concentration in the test bottles was 1.6 g/L

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test conditions

Nominal and measured concentrations:

nominal: 99.5 and 102 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL BOD flasks with gas inlet
- Biomass loading rate: 1.6 g/L activated sludge

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : respiration rate

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

3 h

Dose descriptor:

other: IC20

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

3 h

Dose descriptor:

other: IC80

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- Relevant effect levels: IC50 = 11 mg/l

Any other information on results incl. tables

Since the observed effect on the respiration rate was less than 10% at 100 mg/L, no lower test concentrations were tested