Benzoic acid, 3,3'-[(2,5-dimethyl-1,4-phenylene)bis[imino(1-acetyl-2-oxo-2,1-ethanediyl)azo]]bis[4-chloro-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non GLP guideline study without analytical monitoring; test duration of 24h instead of 48h; due to low water solubility an emulsifier has been used

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

, test duration of 24h instead of 48h

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-[(2E)-2-[1-({4-[(2E)-2-[2-(5-carboxy-2-chlorophenyl)hydrazin-1-ylidene]-3-oxobutanamido]-2,5-dimethylphenyl}carbamoyl)-2-oxopropylidene]hydrazin-1-yl]-4-chlorobenzoic acid

EC Number:

616-083-7

Cas Number:

74351-78-3

Molecular formula:

C30 H26 Cl2 N6 O8

IUPAC Name:

3-[(2E)-2-[1-({4-[(2E)-2-[2-(5-carboxy-2-chlorophenyl)hydrazin-1-ylidene]-3-oxobutanamido]-2,5-dimethylphenyl}carbamoyl)-2-oxopropylidene]hydrazin-1-yl]-4-chlorobenzoic acid

Details on test material:

- Name of test material (as cited in study report): TK 10114/ZP
- Physical state: brown powder
- Analytical purity: commercial grade
- Lot/batch No.: OP 31
- Expiration date of the lot/batch: 1992-03
- Storage condition of test material: at room temperature

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 200 mg TK 10114/ZP and 8 mg alkylphenol-polyglycolether were mixed with and made up to 2000 mL with water
- Controls: yes
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Alkylphenol-polyglycolether
- Concentration of vehicle in test medium (stock solution and final test solution):
- Evidence of undissolved material (e.g. precipitate, surface film, etc): The test substance appeared homogenously distributed in all test concentrations

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Great waterflea
- Source: in-house culture
- Age at study initiation (mean and range, SD): 0 - 24 hours
- Method of breeding: Parthenogenetic reproduction. Feed: Suspension of green algae supplemented by trout chow and/or powder milk and/or yeast; amount of food is consumed after 24 hours
- Feeding during test: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Test conditions

Hardness:

240 mg/L CaCO3

Test temperature:

20 ± 1 °C

pH:

7.6 - 7.9

Dissolved oxygen:

no data

Nominal and measured concentrations:

nominal: control, vehicle control, 10, 18, 32, 58, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Beakers covered with watch glasses
- Material, size, headspace, fill volume: 100 mL fill volume
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water
- Intervals of water quality measurement: at test start and end

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light
- Light intensity: approximately 2000 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility

Results and discussion

Effect concentrations

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

At a test concentration of 32 mg/L a single daphnid was immobile after 24 hours. However, since there were no immobile individuals in the higher test concentrations of 50 and 100 mg/L, the effect is not considered as test substance related.