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Diss Factsheets
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EC number: 810-760-2 | CAS number: 149855-64-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP compliant study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guideline
- Guideline:
- other: EpiOcular
- Principles of method if other than guideline:
- MatTek Corporation, Ashland, MA 01721, USA: EpiOcularTM human cell construct: Procedure details, Version 3.1a of February 10, 2010.
- Harbell J.W. et al. (2009): COLIPA Program on Optimization of Existing In Vitro Eye Irritation Assays for Entry into Formal Validation: Technology Transfer and Intra/Inter Laboratory Evaluation of EpiOcular Assay for Chemicals. Poster # 378, Society of Toxicology, March 2009. - GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-propylheptyl methacrylate
- EC Number:
- 810-760-2
- Cas Number:
- 149855-64-1
- Molecular formula:
- C14 H26 O2
- IUPAC Name:
- 2-propylheptyl methacrylate
- Reference substance name:
- 2-Propylheptyl methacrylate
- IUPAC Name:
- 2-Propylheptyl methacrylate
- Details on test material:
- - Name of test material (as cited in study report): 2-Propylheptyl methacrylate
- Physical state: liquid
- Analytical purity: 94.4 g/100g
- Lot/batch No.: S731900107
Constituent 1
Constituent 2
Test animals / tissue source
- Species:
- other: in vitro
- Strain:
- other: in vitro
Test system
- Vehicle:
- unchanged (no vehicle)
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 50 µl - Duration of treatment / exposure:
- 30 min
- Observation period (in vivo):
- 2-hours post incubation period
- Details on study design:
- TEST SYSTEM
The EpiOcular™ model (OCL-200) is a three-dimensional non-keratinized tissue construct composed of normal human derived epidermal keratinozytes used to model the human corneal epithelium. The EpiOcular™ tissues (surface 0.6 cm2)are cultured on specially prepared cell culture inserts (MILLICELLs®, 10 mm Ø ) and are commercially available as kits (EpiOcular™ 200), containing 24 tissues on shipping agarose.
EXPERIMENT AL PROCEDURE
Two tissues are treated with each test substance, the PC and NC, respectively. There are two separate protocols for liquids and solids, differing in exposure time and post-incubation period. Due to the physical condition of the test substance the protocol for solids is applied.
The tissues will be transferred to sterile 6-well plates with 1 ml pre-warmed assay medium and preconditioned in the incubator at 37°C for 16 - 24 hours. After the pre-incubation the tissues are pre-treated with 20 μl of PSS in order to wet the tissue surface. The tissues are incubated at standard culture conditions for 30 minutes.
After the incubation I postincubation period, the assay medium is replaced by 0.3 ml MTT solution and the tissues are incubated in the incubator for 3 hours. After incubation, the tissues are washed with PBS to stop the MTT-incubation.
The formazan that is metabolically produced by the tissues will be extracted by incubation of the tissues in 2 ml isopropanol at room temperature overnight or for at least 2 hours on a plate shaker (ea. 120 rpm). After shaking the isopropanol extract and piercing the tissues, 2 aliquots of each extract per tissue will be transferred to a 96-well microtiter plate. The optical density (OD570) will be determined spectrophotometrically using a filter with a wavelength of 570 nm.
Results and discussion
Any other information on results incl. tables
Individual and mean OD570 values, individual and mean viability values and inter-tissue variability
Test substance |
|
tissue 1 |
tissue 2 |
mean |
inter-tissue variability [%] |
NC |
mean OD570 |
1.960 |
1.845 |
1.903 |
|
viability [% of NC] |
103.0 |
97.0 |
100 |
6.0 |
|
13/0488 |
mean OD570 |
1.855 |
1.877 |
1.866 |
|
viability [% of NC] |
97.5 |
98.7 |
98 |
1.2 |
|
PC |
mean OD570 |
0.523 |
0.555 |
0.539 |
|
viability [% of NC] |
27.5 |
29.2 |
28 |
1.7 |
Mean tissue viability (%of negative control) |
Prediction
|
≤50 |
Irritant |
>50≤60 |
no prediction* |
>60 |
non-irritant |
*At presentnoprediction isperformedif the mean relative tissue viability with a test
materialis> 50 ≤ 60%as the cut off valueiscurrently being evaluated toliein this range.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.