Benzoic acid, 3,3'-[(2,5-dimethyl-1,4-phenylene)bis[imino(1-acetyl-2-oxo-2,1-ethanediyl)azo]]bis[4-chloro-

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study without analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-[(2E)-2-[1-({4-[(2E)-2-[2-(5-carboxy-2-chlorophenyl)hydrazin-1-ylidene]-3-oxobutanamido]-2,5-dimethylphenyl}carbamoyl)-2-oxopropylidene]hydrazin-1-yl]-4-chlorobenzoic acid

EC Number:

616-083-7

Cas Number:

74351-78-3

Molecular formula:

C30 H26 Cl2 N6 O8

IUPAC Name:

3-[(2E)-2-[1-({4-[(2E)-2-[2-(5-carboxy-2-chlorophenyl)hydrazin-1-ylidene]-3-oxobutanamido]-2,5-dimethylphenyl}carbamoyl)-2-oxopropylidene]hydrazin-1-yl]-4-chlorobenzoic acid

Details on test material:

- Name of test material (as cited in study report): TK 10114/ZP
- Physical state: brown powder
- Analytical purity: commercial grade
- Lot/batch No.: OP 31
- Expiration date of the lot/batch: 1992-03
- Storage condition of test material: at room temperature

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item was directly added to the test adquaria
- Controls: yes
- Evidence of undissolved material (e.g. precipitate, surface film, etc): A precipitation of the test substance was observed from the start of the test

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra-fish
- Source: West Aquarium, Bad Lauterberg, Germany
- Length at study initiation (length definition, mean, range and SD): 24 mm (20 - 29 mm)
- Weight at study initiation (mean and range, SD): 0.11 g (0.06 - 0.17 g)
- Feeding during test: no

ACCLIMATION
- Acclimation period: 40 days

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

188 mg CaCO3/L

Test temperature:

23 ± 1 °C

pH:

7.3 - 8.2

Dissolved oxygen:

80 - 102% saturation

Nominal and measured concentrations:

nominal: control, 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 L glass aquaria
- Material, size, headspace, fill volume: fill volume of 15 L
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.07 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water
- Intervals of water quality measurement: after 0, 24, 48, 72 and 96 hours

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)