Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 267-956-0 | CAS number: 67953-76-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_environmental-fate-and-pathways.png)
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Equivalent to a guideline study. Test procedure in accordance with generally accepted scientific standards and described in sufficient detail, apart from the temperature and some pages missing from the report as presently available.
- Principles of method if other than guideline:
- Method: other
- GLP compliance:
- no
- Radiolabelling:
- yes
- Vehicle:
- no
- Details on preparation of test solutions, spiked fish food or sediment:
- Test Medium: Water drawn from a 125 m bedrock well
STOCK AND TEST SOLUTION AND THEIR PREPARATION: Stock solutions were prepared by diluting known amounts of labelled and unlabelled test substance with distilled water. Diluter stock solutions were prepared by further dilution with distilled water. Test solutions were prepared using an intermittent-flow proprtional diluter.
TEST CONCENTRATIONS: 0.06 and 0.6 mg/l nominal (0.058 ± 0.01; 0.55 ± 0.16 mg/l measured) - Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISMS: Size: 19 ± 2 cm Weight: 39 ± 3 g Pretreatment: acclimated for 30 days before testing. Cumulative mortality less than 2% during holding period Feeding: Dry pelleted ration twice daily ad libitum
- Route of exposure:
- aqueous
- Total exposure / uptake duration:
- 49 d
- Total depuration duration:
- 14 d
- Hardness:
- Hardness: 35 mg/l as CaCO3
- Test temperature:
- Temperature: 18 ± 1.0 °C
- pH:
- pH: 7.1
- Dissolved oxygen:
- Dissolved Oxygen Concentration: > 6.0 mg/l (60% saturation)
- Details on estimation of bioconcentration:
- ENDPOINTS: Bioaccumulation, elimination rate over a 14 day depuration period, and distribution between edible and non-edible tissues.
- Conc. / dose:
- 0.06 mg/L
- Type:
- BCF
- Value:
- 71 dimensionless
- Basis:
- non-edible fraction
- Remarks on result:
- other: Conc.in environment
- Conc. / dose:
- 0.06 mg/L
- Type:
- BCF
- Value:
- < 7 dimensionless
- Basis:
- whole body d.w.
- Remarks on result:
- other: Conc.in environment
- Conc. / dose:
- 0.6 mg/L
- Type:
- BCF
- Value:
- 31 dimensionless
- Basis:
- non-edible fraction
- Remarks on result:
- other: Conc.in environment
- Conc. / dose:
- 0.6 mg/L
- Type:
- BCF
- Value:
- < 2 dimensionless
- Basis:
- whole body d.w.
- Remarks on result:
- other: Conc.in environment
- Elimination:
- yes
- Parameter:
- other: After 14 days depuration, carp that had been exposed to 0.058 and 0.55 mg/l test substance had eliminated all measurable 14-C residues.
- Depuration time (DT):
- 14 d
- Details on results:
- BIOACCUMULATION AT 0.06 mg/l NOMINAL TEST CONCENTRATION: A mean fish concentration of 4.1 mg/kg, equivalent to BCF = 71, was determined for non-edible tissues, although no meaningful trends in bioaccumulation were observed at 7 sampling points over the exposure period.
Analysis of the gut, faeces and visceral tissues at Day 42 showed that 94% of residues were present in faecal matter, 1% in the gut and 5% in the remaining visceral tissues, indicating that the substance was ingested and eliminated with very little accumulation.
Expressed on a whole-body basis, the mean fish concentration was < 0.41 mg/kg, equivalent to BCF < 7.
BIOACCUMULATION AT 0.6 mg/l NOMINAL TEST CONCENTRATION: A similar pattern to the lower test concentration was observed.
The mean fish concentration determined for non-edible tissues, equivalent to BCF = 31. Analysis on Day 42 showed 83% in faecal matter, 2% in the gut and 15% in the remaining visceral tissues.
Expressed on a whole-body basis, the mean fish concentration was < 1.0 mg/kg, equivalent to BCF < 2.
Measured values close to limits of detection, especially for whole body. - Conclusions:
- The test substance did not bioaccumulate in the edible
tissues of carp. Some accumulation of the test substance was
observed in the visceral tissues, although analysis revealed that most of this was associated with ingesta and faecal
material, indicating that the the substance was ingested and eliminated with very little accumulation. - Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to Annex 3 of the CSR and IUCLID Section 13 for justification of read-across between members of the HEDP category.
- Reason / purpose for cross-reference:
- read-across source
- Conc. / dose:
- 0.06 mg/L
- Type:
- BCF
- Value:
- 71 dimensionless
- Basis:
- non-edible fraction
- Remarks on result:
- other: concentration in environment
- Conc. / dose:
- 0.06 mg/L
- Type:
- BCF
- Value:
- < 7 dimensionless
- Basis:
- whole body d.w.
- Remarks on result:
- other: concentration in environment
- Conc. / dose:
- 0.6 mg/L
- Type:
- BCF
- Value:
- 31 dimensionless
- Basis:
- non-edible fraction
- Remarks on result:
- other: concentration in environment
- Conc. / dose:
- 0.6 mg/L
- Type:
- BCF
- Value:
- < 2 dimensionless
- Basis:
- whole body w.w.
- Remarks on result:
- other: concentration in environment
- Elimination:
- yes
- Parameter:
- other: After 14 days depuration, carp that had been exposed to 0.058 and 0.55 mg/l test substance had eliminated all measurable 14-C residues.
- Depuration time (DT):
- 14 d
Referenceopen allclose all
Description of key information
A reliable study of bioaccumulation of HEDP-H in Cyprinus carpio indicates a bioconcentration factor of 71 for non-edible tissues (concentration level 0.06 mg/L, nominal). When expressed in terms of a whole body basis, the BCF is equivalent to <7. At a concentration level of 0.6 mg/L, a BCF value of 31 was determined for non-edible tissues, which is equivalent to <2 when expressed in terms of a whole body basis.
Key value for chemical safety assessment
- BCF (aquatic species):
- 71 dimensionless
Additional information
- HEDP is present as HEDP-H or one of its ionised forms. The degree of ionisation depends upon the pH of the system and not whether HEDP (1-2Na), HEDP (2-3Na), HEDP-4Na, HEDP-xK salts, HEDP-H or another salt was added.
- Disassociated sodium/potassium cations. The amount of sodium/potassium present depends on which salt was added.
- Divalent and trivalent cations have much higher stability constants for binding with HEDP than the sodium or potassium ions, so would preferentially replace them. These ions include calcium (Ca2+), magnesium (Mg2+) and iron (Fe3+). Therefore, the presence of these in the environment or in biological fluids or from dietary sources would result in the formation of HEDP-dication (e.g. HEDP-Ca, HEDP-Mg) and HEDP-trication (e.g. HEDP-Fe) complexes in solution, irrespective of the starting substance/test material.
A reliable aquatic bioaccumulation study has been read-across from HEDP-H (EG&G, 1976). In this study, BCF values of 71 and 31 for the concentration levels of 0.06 mg/L and 0.6 mg/L were determined for non-edible tissues. These values are equivalent to <7 and <2 when expressed in terms of a whole body basis. The study was conducted according to generally accepted scientific principles and was assigned a reliability rating of 2.
A supporting study with HEDP (2-3Na) reported extensive elimination of the test substance after 2 weeks in Danio rerio, reporting a BCF <1 (Steber and Wierich, 1986). In the same study, a steady state BCF was calculated on the basis of the uptake and clearance rate constants, which was BCF = 17.9. However, based on a lack of documentation, the study was assigned a reliability rating of 4.
The acid, sodium and potassium salts in the HEDP category are freely soluble in water and, therefore, the HEDP anion is fully dissociated from its sodium or potassium cations when in solution. Under any given conditions, the degree of ionisation of the HEDP species is determined by the pH of the solution. At a specific pH, the degree of ionisation is the same regardless of whether the starting material was HEDP-H, HEDP (1-2Na), HEDP (2-3Na), HEDP-4Na, HEDP-xK or another salt of HEDP.
Therefore, when a salt of HEDP is introduced into test media or the environment, the following is present (separately):
In this context, for the purpose of this assessment, read-across of data within the HEDP Category is considered to be valid.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)