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EC number: 242-440-8 | CAS number: 18599-22-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23 October 2020 to 22 March 2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 021
- Report date:
- 2021
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 4-bromo-3,3,4,4-tetrafluorobut-1-ene
- EC Number:
- 242-440-8
- EC Name:
- 4-bromo-3,3,4,4-tetrafluorobut-1-ene
- Cas Number:
- 18599-22-9
- Molecular formula:
- C4H3BrF4
- IUPAC Name:
- 4-bromo-3,3,4,4-tetrafluorobut-1-ene
- Test material form:
- liquid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): collected from the Easton Wastewater Treatment Facility which treats predominantly residential wastes.
- Preparation of inoculum for exposure: The sludge was sieved and then aerated at test temperature until use.
- Concentration of sludge: After sieving, adjusted to approximately 1000 mg total suspended solids/L with mineral media.
- Type and size of filter used, if any: 2mm screen
A total suspended solids measurement and standard plate count were performed on the inoculum on the day of use in the test.
Plates were incubated at 22 ± 2 ºC for approximately 48 hours - Duration of test (contact time):
- 29 d
Initial test substance concentration
- Initial conc.:
- 10 other: mg C/L
- Based on:
- test mat.
- Remarks:
- 0.1293 g (%C calculated as 23.21%) added to test chamber with final volume 3000 mL
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Prepared by adding, per litre of high quality water: 1 mL calcium chloride solution (2.75%), 1 mL of ferric chloride solution (0.025%), 1 mL of magnesium sulphate solution (2.25%) and 10 mL of phosphate buffer solution (pH 7.4)
- Test temperature: 20-22ºC, with the average test temperature as 21 ºC.
- pH: 7.50
- pH adjusted: No
- Aeration of dilution water: No
- Suspended solids concentration: ≤30 mg/L
TEST SYSTEM
- Number of culture flasks/concentration: three replicates
- Method used to create aerobic conditions: bubbling CO2-free air through sludge
- Measuring equipment: Shimadzu Model TOC-VCSH carbon analyzer.
- Details of trap for CO2 and volatile organics if used: Three traps, containing KOH solution to convert CO2 to K2CO3.
SAMPLING
- Sampling frequency: days 3, 6, 9, 12, 15, 20, 25 and 29
- Sampling method: the CO2 trap nearest the test chamber was collected for analysis of inorganic carbon. The two remaining traps were placed one position closer to the test chamber and a new trap was placed on the end of the series.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Abiotic sterile control: No
- Toxicity control: Sodium benzoate/test material (10/10 mg C/L)
- Other: Sodium benzoate (10 mg C/L)
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- < 0
- St. dev.:
- 1.2
- Sampling time:
- 29 d
- Details on results:
- The actual result at day 29 varied between -0.6 to -2.9% for the three replicates. The Cumulative average at day 29 was -1.6% degradation. The highest % degradation value observed at any sampling point during the study, was 3.1% at day 9 but the other two replicates taken on same day were both negative percentages.
BOD5 / COD results
- Results with reference substance:
- An average percent biodegradation of greater than 60% was achieved by Day 6, thereby fulfilling the criteria for a valid test by reaching the pass level by Day 14.
Any other information on results incl. tables
Table 1 Dissolved Organic Carbon (DOC) and pH of test solutions at test termination
Test Chamber (Nominal concentration) | DOC (mg C/L) | pH |
Control Rep. 1 | 2.0 | 7.46 |
Control Rep. 2 | 1.3 | 7.48 |
Control Rep. 3 | 1.2 | 7.47 |
Sodium Benzoate Rep. 1 (10 mg C/L) | 1.4 | 7.55 |
Sodium Benzoate Rep. 2 (10 mg C/L) | 1.5 | 7.57 |
Sodium Benzoate Rep. 3 (10 mg C/L) | 1.4 | 7.56 |
Test material Rep. 1 (10 mg C/L) | 8.1 | 7.50 |
Test material Rep. 1 (10 mg C/L) | 7.5 | 7.50 |
Test material Rep. 1 (10 mg C/L) | 7.3 | 7.50 |
Toxicity control (sodium benzoate/test item (10/10 mg C/L) | 7.8 | 7.61 |
Table 2 Measured inorganic carbon concentration of trapping solutions (mg C/L)
|
|
Blank |
Blank |
Blank |
Sodium |
Sodium |
Sodium |
|
|
|
Toxicity Control |
|
Date |
Day |
Control |
Control |
Control |
Benzoate |
Benzoate |
Benzoate |
BTFB |
BTFB |
BTFB |
BTFB/ |
KOH |
|
|
Rep. 1 |
Rep. 2 |
Rep. 3 |
Rep. 1 |
Rep. 2 |
Rep. 3 |
Rep. 1 |
Rep. 2 |
Rep. 3 |
Sodium Benzoate |
Blank |
1-Nov-20 |
3 |
38.5 |
36.0 |
37.8 |
179.0 |
187.8 |
202.1 |
35.0 |
36.6 |
36.5 |
96.6 |
8.2 |
4-Nov-20 |
6 |
39.5 |
42.3 |
44.1 |
130.5 |
112.3 |
104.8 |
35.4 |
38.9 |
51.1 |
150.3 |
6.9 |
7-Nov-20 |
9 |
39.6 |
39.2 |
37.4 |
64.0 |
57.3 |
62.2 |
38.2 |
35.5 |
39.7 |
85.8 |
7.3 |
10-Nov-20 |
12 |
29.6 |
32.8 |
30.6 |
41.4 |
42.4 |
44.6 |
29.3 |
31.6 |
30.5 |
51.3 |
7.6 |
13-Nov-20 |
15 |
31.8 |
28.3 |
30.2 |
35.1 |
40.6 |
37.9 |
26.1 |
31.7 |
29.2 |
41.2 |
10.0 |
18-Nov-20 |
20 |
26.8 |
29.4 |
25.3 |
27.7 |
27.2 |
29.5 |
35.8 |
33.5 |
27.4 |
35.9 |
11.7 |
22-Nov-20 |
25 |
24.0 |
25.6 |
29.8 |
25.1 |
23.9 |
27.5 |
24.0 |
21.9 |
22.1 |
27.9 |
10.3 |
27-Nov-20 |
29 |
19.9 |
19.5 |
20.2 |
17.8 |
16.3 |
17.9 |
20.4 |
19.2 |
14.7 |
17.4 |
6.8 |
Table 3 Cumulative milligrams of carbon dioxide evolved1,2
Date | Day | Blank | Blank | Blank | Sodium | Sodium | Sodium | Tox Control | KOH | |||
Control | Control | Control | Benzoate3 | Benzoate3 | Benzoate3 | BTFB3 | BTFB3 | BTFB3 | BTFB/ | Blank | ||
Rep. 1 | Rep. 2 | Rep. 3 | Rep.1 | Rep.2 | Rep.3 | Rep. 1 | Rep. 2 | Rep. 3 | Sodium Benzoate | |||
1-Nov-20 |
3 |
14.1 |
13.2 |
13.9 |
52.0 |
55.2 |
60.4 |
-0.9 |
-0.3 |
-0.3 |
21.7 |
3.0 |
4-Nov-20 |
6 |
28.6 |
28.8 |
30.1 |
84.4 |
81.0 |
83.5 |
-3.3 |
-1.5 |
3.0 |
61.5 |
5.5 |
7-Nov-20 |
9 |
43.1 |
43.1 |
43.8 |
93.7 |
87.8 |
92.1 |
-3.5 |
-2.6 |
3.4 |
78.8 |
8.2 |
10-Nov-20 |
12 |
54.0 |
55.2 |
55.1 |
97.5 |
92.0 |
97.1 |
-4.1 |
-2.4 |
3.2 |
86.2 |
11.0 |
13-Nov-20 |
15 |
65.7 |
65.6 |
66.1 |
99.3 |
95.8 |
99.9 |
-5.6 |
-1.9 |
2.8 |
90.2 |
14.7 |
18-Nov-20 |
20 |
75.5 |
76.3 |
75.4 |
99.5 |
95.8 |
100.8 |
-2.4 |
0.4 |
2.9 |
93.4 |
19.0 |
22-Nov-20 |
25 |
84.3 |
85.7 |
86.4 |
99.0 |
94.9 |
101.2 |
-3.4 |
-1.2 |
1.3 |
94.0 |
22.7 |
27-Nov-20 |
29 |
91.6 |
92.9 |
93.8 |
98.3 |
93.6 |
100.4 |
-3.2 |
-1.5 |
-0.6 |
93.1 |
25.2 |
1The results of the inorganic carbon analyses of the CO2
traps were converted to mg CO2 produced using the following equation: mg
CO2 = cumulative result (mg C/L) × vol. of KOH (L) × 3.67 mg CO2/mg C.
2Calculations performed in Excel 2010 full precision mode.
Manual calculations may differ.
3Corrected for the CO2 attributed to the inoculum and the KOH
by subtracting the average amount of CO2 evolved by the controls.
Table 4 Cumulative Percent of theoretical carbon dioxide evolved
|
|
Blank |
Blank |
Blank |
Sodium |
Sodium |
Sodium |
|
|
|
Toxicity Control |
Date |
Day |
Control |
Control |
Control |
Benzoate |
Benzoate |
Benzoate |
BTFB |
BTFB |
BTFB |
BTFB/ |
|
|
1 |
2 |
3 |
Rep. 1 |
Rep. 2 |
Rep. 3 |
Rep. 1 |
Rep. 2 |
Rep. 3 |
Sodium Benzoate |
1-Nov-20 |
3 |
NA |
NA |
NA |
47.2 |
50.1 |
54.9 |
-0.8 |
-0.3 |
-0.3 |
9.9 |
4-Nov-20 |
6 |
NA |
NA |
NA |
76.7 |
73.6 |
75.8 |
-3.0 |
-1.3 |
2.7 |
27.9 |
7-Nov-20 |
9 |
NA |
NA |
NA |
85.1 |
79.7 |
83.7 |
-3.2 |
-2.4 |
3.1 |
35.8 |
10-Nov-20 |
12 |
NA |
NA |
NA |
88.6 |
83.5 |
88.2 |
-3.8 |
-2.2 |
2.9 |
39.1 |
13-Nov-20 |
15 |
NA |
NA |
NA |
90.2 |
87.0 |
90.8 |
-5.1 |
-1.7 |
2.6 |
41.0 |
18-Nov-20 |
20 |
NA |
NA |
NA |
90.4 |
87.0 |
91.6 |
-2.2 |
0.4 |
2.6 |
42.4 |
22-Nov-20 |
25 |
NA |
NA |
NA |
89.9 |
86.2 |
91.9 |
-3.1 |
-1.1 |
1.2 |
42.7 |
27-Nov-20 |
29 |
NA |
NA |
NA |
89.3 |
85.0 |
91.2 |
-2.9 |
-1.3 |
-0.6 |
42.3 |
Cumulative Average Day 29 (n=3) |
|
|
|
88.5 |
|
|
-1.6 |
|
42.3 |
|
|
Standard Deviation |
|
|
|
3.2 |
|
|
1.2 |
|
|
|
|
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- In addition to valid result for positive control, also the toxicity control (containing both BTFB and sodium benzoate) achieved > 25% degradation by Day 14 and therefore BTFB may be considered non-inhibitory at the concentration tested in this study
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The final mean percent biodegradation for BTFB was -1.6% at the end of the test. Evidence of ready biodegradability in a Carbon Dioxide Evolution Test is 60% TCO2 within the 28-day test period.
The negative values for the test substance degradation could happen due to the slight variability between replicates for the test substance and the inoculum controls. A negative result in a test of ready biodegradability does not necessarily mean that the test substance will not be biodegradable under relevant environmental conditions, but that additional testing maybe needed.
The toxicity control achieved > 25% degradation by Day 14 and therefore BTFB may be considered non-inhibitory at the concentration tested in this study.
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