Reaction products of 2-(chloromethyl)oxirane and glycerol

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: well-documented publication, which meets basic scientific principles, however the results of this part of the experiments ist not described suffieciently.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

publication

Title:

The Toxicology of Epoxy Resins

Author:

Hine, C.H., Kodama, J.K., Anderson, H.H., Simonson, D.W. and Wellington, J.S.

Year:

1958

Bibliographic source:

AMA archive s of industrial health / American Medical Association, 17, p. 129-144

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The effects of inhalation of EPON 562 were tested on rats by an eight-hour exposure. Air was saturated with EPON 562 at 20°C and at 30°C, by passage the liquids in two fritted glass bubblers connected in series.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

EPON resins 562 (Polyglycidyl Ether of Substituted Glycerin)

IUPAC Name:

EPON resins 562 (Polyglycidyl Ether of Substituted Glycerin)

Details on test material:

- Name of test material (as cited in study report): EPON 562

Test animals

Species:

rat

Strain:

Long-Evans

Sex:

male

Details on test animals or test system and environmental conditions:

no details given

Administration / exposure

Route of administration:

inhalation

Type of inhalation exposure:

whole body

Vehicle:

air

Duration of exposure:

8 h

Concentrations:

saturated vapour, no further specifications given.

No. of animals per sex per dose:

no details given

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 10 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

The animals were observed for the following 10 days, and those that died were subjected to necropsy. At the end of the observation period, the survivors were killed for necropsy, and sections of their tissues were preserved in 10% formalin for histologic examination.

Statistics:

LD50 values were calculated according to the method of Litchfield and Wilcoxon (1948) or the method of Weil (1952).

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

other: saturated vapour

Based on:

test mat.

Exp. duration:

8 h

Remarks on result:

other: No deaths

Applicant's summary and conclusion

Interpretation of results:

other: it is not possible to judge as the results are not described suffienciently.

Remarks:

Criteria used for interpretation of results: expert judgment

Conclusions:

The study was considered to be of low reliability (reliability Klimisch 3), because of the limited documentation of the methods used and the results obtained. The test materials, EPON 562 and diglycidyl resorcinol, did not induce mortality and treatment-related clinical signs in the dose group treated with saturated vapour. No practical or systemic toxicity hazard could be associated with exposure to the vapors or percutaneous absorption of the resins. Chief effects were signs of local irritation. There was no noticeable species variation amoung the rats, mice or rabbits. However, as the results are not decribed in sufficient detail, the study is not judged to be reliable.

Executive summary:

The acute toxicity of epoxy resins was investigated by Hine et al.(Hine, 1958). The effect of inhalation of the two most fluid resins, EPON 562 and DGR, was tested on rats (male Long evans) and mice (male Swiss webster) by an eight-hour exposure. Air was saturated with EPON 562 at 20±2°C and at 30±1°C, and with DGR at 30±1°C, by passage through the liquids in two fritted glass bubblers connected in series. The animals were observed for the following 10 days, and those that died were subjected to necropsy. At the end of the observation period, the survivors were killed for necropsy, and sections of their tissues were preserved in 10% formalin for histologic examination. They found that neither compound caused death when rats were exposed for eight hours to saturated vapours. The toxicity of the derivative was slightly less than that of the parent compound. Gross pathology was nonspecific, and the chief effect was that of local irritation. There was no noticeable species variation among the rat, mouse, and rabbit.

In conlusion the investigation of the toxicology of certain epoxy resins, a new type of condensation plastic, indicated that the epoxy resins are of a low order of toxicity. The biological activity is considerably less than that of most monomers, and, residing in the epoxy group, disappears on curing of the resins.