Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP Guideline Study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Basic Yellow 851 1
- Analytical purity: 98,9 area-% (HPLC at a wavelength of 215 nm)²; 99,1 area-% (HPLC at a wavelength of 420 nm)²
- Impurities (identity and concentrations): 2,6 % water², 10,6 % chloride²
- Lot/batch No.: Lab.-J .-Nr. ZD 00969/086

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Laboratory culture: yes, Municipal activated sludge taken from a laboratory wastewater treatment plant fed with municipal sewage.
- Preparation of inoculum for exposure: The inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated
for 24 hours. 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/L dry substance in the test.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
180 min

Test conditions

Test temperature:
20 ± 2 °C
pH:
6.8 - 7.2
Dissolved oxygen:
Oxygen concentration during aeration: > 2,5 mg/l
Oxygen concentration immediately before measurement: > 6,5 mg/l
Nominal and measured concentrations:
Nominal concentrations: 1, 10, 100, 500, 1000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (nominal volume 250 ml)
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Synthetic medium: 8 ml/vessel 100-fold concentrated OECD medium
Reference substance (positive control):
yes
Remarks:
3,5 dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
180 min
Dose descriptor:
other: EC20
Effect conc.:
ca. 24 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
EC50
Effect conc.:
ca. 140 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
other: EC80
Effect conc.:
ca. 390 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Applicant's summary and conclusion