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EC number: 237-438-9
CAS number: 13784-51-5
The revised OECD guideline of 2006 contains some additional validity
criteria. These criteria have been verified for this study and are found
to be within the limits given in the guideline. See for details the
In order to predict effects of chemicals in
an aquatic environment, the toxicity to freshwater algae was assessed.
The algal toxicity was determined in the Algal Growth Inhibition test in
accordance with OECD, EEC and ISO test guidelines and with the OECD
Principles of Good Laboratory Practice. The guidelines were slightly
modified to ensure good growth and pH control of the cultures.
The green algae Pseudokirchneriella
subcapitata (formerly known as Selenastrum capricomutum) was exposed to
the following concentrations: 4.30, 9.37, 20.76, 45.58 and 100.01 mg/L
of acetyl acetone peroxide in test medium.
The chemical analyses performed on the
samples taken during the test demonstrated that the test substance is
not stable during the test. At the end of the test only 52% of the
initial concentration was found at the highest test concentration.
Despite the instability of the parent compound, the effects are all
calculated based on the concentration of parent compound as measured at
the beginning of the test.
The toxicity of acetyl acetone peroxide to
exponentially growing culture of Pseudokirchneriella subcapitata was
determined over an exposure period of 72 hours. The EbC50 and ErC50
(0-72 h) values of the test substance as calculated from the dosis
effect curve for P. subcapitata are 18.71 mg/l and 27.91 mg/L (22.10 -
36.78 mg/l, 95% confidence limits), respectively. The NOEC determined
from the results is 9.37 mg/L, the LOEC is 20.76 mg/L. The test was
conducted in a mineral salts medium in a climatized illuminated orbital
incubator. The maximum variation in pH in the test media was 0.8 pH
The definitive test is valid as shown by the
increase of the extinction of the control over 72 h by a factor of 134.
Remark from the reviewer: The results given
in this summary are as given in the report, so have not been converted
to TWA active ingredient concentrations.
72h-ErC10 for freshwater algae
is 3.5 mg a.i./L (measured. TWA) and the 72h-ErC50 is 5.4 mg a.i./L
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