4,4'-methylenedicyclohexyl diisocyanate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Monitoring of algae media was performed with DOC. No substance specific analysis was performed.

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

To accelerate the solution procedure a five fold amount (45 mg/1) of the maximum water solubility of the test substance in the
preliminary test (9 mg/1) was weighed into water, treated for 60 seconds at 8000 rpm.with an ultra-turrax and afterwards stirred
an a magnetic stirrer for 24 hours. To remove undissolved particles, the resulting emulsion was filtered using a folded filter of the
pore size 7-12 gm.

Test organisms (species):

other: Daphnia magna STRAUSS

Details on test organisms:

TEST ORGANISM:
- Parthenogenetic females, age 0 – 24 hours, laboratory bred at BAYER AG Leverkusen, cloned at the Federal Health Office (BGA), Berlin Germany
- Water for aquaculture: M4-medium according to Elendt, version of BGA (1992), originally described in Water Research 24 (9), Sept. 1990; 1157-1167

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

14.2 °dH (253.47 mg/l CaCO3)

Test temperature:

20.2 °C (after 48 hrs)

pH:

7.9 (after 48 hrs)

Dissolved oxygen:

8.5 mg/l (after 48 hrs)

Salinity:

No data

Nominal and measured concentrations:

Nominal concentration of test substance: 9 mg/l (Analytical value after 0 hour: 5 mg/l, after 48 hours: 6 mg/l, resp.)

Details on test conditions:

TEST SYSTEM:
- water for dilution: M4 -Medium according to Elendt and BGA (1992)
- control: M4 -Medium according to Elendt and BGA (1992)
- photoperiod: 16 hours light, 8 hours dark (illumination 1000 lux)
- cylindric test vessel: diameter 4.0 cm/height 6.5 cm, 20 ml test medium,not ventilated
- number of daphnids per beaker: 10 daphnids (neonates)
- test parameter: immobilization (death)
- method of administration: direct weighing
- test concentration: 1 (9 mg/l) plus 1 control
- replicate: 2 (per concentration/control)
- chemical analysis: TOC

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 8.3 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

other: determined as TOC and back-calculated to the parent compound

Basis for effect:

mobility

Remarks on result:

other: Arithmetic mean of analytical values

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

>= 9 mg/L

Nominal / measured:

nominal

Conc. based on:

other: determined as TOC and back-calculated to the parent compound

Basis for effect:

mobility

Details on results:

The above test result refers to a concentration of the test substance which was calculated directly from analytically determined TOC values.

Validity criteria fulfilled:

yes

Executive summary:

The acute toxicity of 4,4-methylenedicyclohexyl diisocyanate to Daphnia magna is EC 0 >= 8.3 mg/l at 48 h (Bayer 2000). The result is expressed in terms of arithmetic mean of analytical values. The study was conducted according to the Council Directive 92/69/EEC C.2 (1992).

Description of key information

With the invertebrate Daphnia magna one acute static limit test in accordance with Directive 92/69/EEC, C.2, is available. For a test period of 48 hours an EC50 value of > 8.3 mg/l was determined. At this concentration no mortality was observed (Bayer AG, 2000c).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

8.3 mg/L

Additional information

Should read >8.3 mg/L