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EC number: 248-420-5 | CAS number: 27344-06-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The test procedure cannot be subsumed under a testing guideline, nevertheless are scientifically acceptable.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 975
- Report date:
- 1975
Materials and methods
- Principles of method if other than guideline:
- Acute percutaneous toxicity test.
The acute dermal toxicity of the test item was investigated in male rats, in a 40 % of aqueous gum tragacanth. 12.5 ml of the substance was spreaded dermally and covered with an aluminum foil. After the exposure time of 24 hours, mortalities, signs of toxicity and dermal toxicity were observed. - GLP compliance:
- no
- Remarks:
- Pre GLP.
Test material
- Reference substance name:
- Disodium 4,4'-bis[[4-anilino-6-[(2-carbamoylethyl)(2-hydroxyethyl)amino]-1,3,5,-triazin-2-yl]amino]stilbene-2,2'-disulphonate
- EC Number:
- 248-420-5
- EC Name:
- Disodium 4,4'-bis[[4-anilino-6-[(2-carbamoylethyl)(2-hydroxyethyl)amino]-1,3,5,-triazin-2-yl]amino]stilbene-2,2'-disulphonate
- Cas Number:
- 27344-06-5
- Molecular formula:
- C42H46N14O10S2.2Na
- IUPAC Name:
- disodium 4,4'-bis[[4-anilino-6-[(2-carbamoylethyl)(2-hydroxyethyl)amino]-1,3,5,-triazin-2-yl]amino]stilbene-2,2'-disulphonate
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: CFY
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 229 - 249 g.
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- other: aqueous gum tragacanth (0.5 %)
- Details on dermal exposure:
- TEST CONCENTRATION
- Dose concentration: 12.5 ml/kg at 40 % of substance.
TEST SITE
- Area of exposure: dorso-lumbar region, 10 % of the total body surface.
- Hair removing method: electric clippers. No shaving or chemical depilation.
- Day of the preparation: one day prior to treatment.
- Type of wrap if used: with "Sleek" waterproof plaster, firmly round the trunk.
REMOVAL OF TEST SUBSTANCE
- Washing with warm diluted soap solution (40-50 °C), rinsing in clean warm water and blotting dry with absorbent paper. - Duration of exposure:
- 24 hours.
- Doses:
- 0 and 5000 mg/l.
- No. of animals per sex per dose:
- 5 males per dose.
- Control animals:
- yes, concurrent no treatment
- Details on study design:
- - Duration of observation period following administration: 14 days.
- Observations: daily, for signs of of dermal irritation, according to the scoring system. All animals were sacrificed terminally and examined macroscopically in an attempt to identify any target organs.
Results and discussion
Effect levels
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- > 5 000 mg/kg bw
- Mortality:
- No mortalities were found.
- Clinical signs:
- other: No signs of toxicity were observed.
- Gross pathology:
- Normal.
- Other findings:
- No dermal reactions were observed.
Any other information on results incl. tables
Results
Sex | Dosage level | Body weight (g) at | Mortality | ||
Male | (g/kg) | Dosing | 1 week | 2 weeks | |
0 | 249 | 327 | 382 | 0/5 | |
5 | 243 | 270 | 318 | 0/5 |
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information according to the CLP regulation Criteria used for interpretation of results: EU
- Conclusions:
- LD50 (percutaneous) > 5000 mg/l, non toxic.
- Executive summary:
Method
The acute dermal toxicity of the test item was investigated in male rats, in a 40 % of aqueous gum tragacanth. 12.5 ml of the substance was spreaded dermally and covered with an aluminum foil. After the exposure time of 24 hours, mortalities, signs of toxicity and dermal toxicity were observed.
Results
The percutaneous LD50 was > 5000 mg/l, therefore the test item could be considered non toxic.
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