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EC number: 203-176-9
CAS number: 104-12-1
Topical application of a 0.1 ml aliquot of the substance to
the clipped and depilated backs of 10 guinea pigs per test
material 4 times in 10 days. At the time of the third
application 0.2 ml of Freund's adjuvant was injected
intradermally at one point adjacent to the insult site.
After a 2-week rest period, the guinea pigs were challenged
on the clipped flanks with the test material on one flank.
The challenge site was evaluated for erythema and edema at
24 and 48 hours.
No guinea pig (0/10) showed a positive result, whereas the
positive control was functional.
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