4-chlorophenyl isocyanate

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-chlorophenyl isocyanate

EC Number:

203-176-9

EC Name:

4-chlorophenyl isocyanate

Cas Number:

104-12-1

Molecular formula:

C7H4ClNO

IUPAC Name:

1-chloro-4-isocyanatobenzene

Details on test material:

IUCLID4 Test substance: other TS: p-chlorophenyl isocyanate, no data on purity

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Test system

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

dosage: 0.1 g

Duration of treatment / exposure:

no information if eyes were rinsed after application of test substance

Observation period (in vivo):

21 days

Number of animals or in vitro replicates:

6

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

chemosis score

Basis:

mean

Time point:

other: 1 hour

Score:

3

Max. score:

4

Reversibility:

not reversible

Results 2

Irritation parameter:

chemosis score

Basis:

mean

Time point:

other: 24 hours

Score:

3.83

Max. score:

4

Reversibility:

not reversible

Results 3

Irritation parameter:

chemosis score

Basis:

mean

Time point:

other: 48 hours

Score:

4

Max. score:

4

Reversibility:

not reversible

Results 4

Irritation parameter:

chemosis score

Basis:

mean

Time point:

other: 72 hours

Score:

4

Max. score:

4

Reversibility:

not reversible

Results 5

Irritation parameter:

iris score

Basis:

mean

Time point:

other: 1 hour

Score:

0

Max. score:

2

Reversibility:

not reversible

Results 6

Irritation parameter:

iris score

Basis:

mean

Time point:

other: 24 hours

Score:

1

Max. score:

2

Reversibility:

not reversible

Results 7

Irritation parameter:

iris score

Basis:

mean

Time point:

other: 48 hours

Score:

1

Max. score:

2

Reversibility:

not reversible

Results 8

Irritation parameter:

iris score

Basis:

mean

Time point:

other: 72 hours

Score:

1

Max. score:

2

Reversibility:

not reversible

Results 9

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

other: 1 hour

Score:

0

Max. score:

4

Reversibility:

not reversible

Remarks on result:

other: degree of opacity

Results 10

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

other: 24 hours

Score:

1.66

Max. score:

4

Reversibility:

not reversible

Remarks on result:

other: degree of opacity

Results 11

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

other: 48 hours

Score:

1.66

Max. score:

4

Reversibility:

not reversible

Remarks on result:

other: degree of opacity

Results 12

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

other: 72 hours

Score:

2

Max. score:

4

Reversibility:

not reversible

Remarks on result:

other: degree of opacity

Any other information on results incl. tables

RS-Freetext:
Redness (up to score 3 of max.3) and chemosis (up to score 4
of max.4) appeared on day 1; discharge (score 2 of 
max.3), congestion of the iris (score 1 of max.2) and
opacity of the cornea (up to score 4 total area of max. 4)
on day 2. These effects were observed in all animals and did
not regress before day 5.
Details of the iris were not visible in 2 animals from day
14 and in 3 animals from day 15.
At the end of the observation period (day 21) chemosis (in
5/6 score 1 of max.4), redness in 2/6 sore 1 and 1/6 score 3
of max. 3) and congestion of the iris persisted for the
majority of the animals (in 3/6 score 1 of max 4 and in 2/6
iris not visible) and opacity in all animals (in 5/6 score 1
one quarter of the area and in 1/6 score 4 total area of
max. 4)

Applicant's summary and conclusion

Interpretation of results:

irritating

Remarks:

Migrated information

Executive summary:

method: the study was conducted according the recommendation of the OECD guideline no 405.

result: Redness and chemosis appeared on day 1, discharge, congestion of the iris and opacity of the cornea on day 2. these reactions were very important and observed in all animals. they did not regress before day 5. details of the iris were not visible in 2 animals from day 14 and in 3 animals from day 15. at the end of the observation period (day 21), chemosis, redness and congestion of the iris persisted for the majority of animals and opacity of cornea in all animals. the compound was considered as irritant.