Registration Dossier
Registration Dossier
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EC number: 801-656-8 | CAS number: 1416808-92-8
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, December 14, 2000.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- (3E)-2-chloro-3-(hydroxymethylidene)cyclohex-1-ene-1-carbaldehyde
- EC Number:
- 801-656-8
- Cas Number:
- 1416808-92-8
- Molecular formula:
- C8H9ClO2
- IUPAC Name:
- (3E)-2-chloro-3-(hydroxymethylidene)cyclohex-1-ene-1-carbaldehyde
- Details on test material:
- Since the substance decomposes in water, aquatic toxicity from the substance itself is unlikely to occur. In accordance with Column 2 adaptation statement of REACH Annex VII, this study would not need to be conducted if there are mitigating factors indicating that aquatic toxicity is unlikely to occur. This experimental determination has been performed to determine whether the decomposition products cause aquatic toxicity.
Constituent 1
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- 24 mg CaCO3/l
- Test temperature:
- 22-23ºC
- pH:
- starting at 7.7 - 8.2 and ending at 7.7 - 7.8
- Salinity:
- NH4Cl 15 mg/l
MgCl2.6H2O 12 mg/l
CaCl2.2H2O 18 mg/l
MgSO4.7H2O 15 mg/l
KH2PO4 1.6 mg/l
FeCl3.6H2O 64 μg/l
Na2EDTA.2H2O 100 μg/l
H3BO3 185 μg/l
MnCl2.4H2O 415 μg/l
ZnCl2 3 μg/l
CoCl2.6H2O 1.5 μg/l
CuCl2.2H2O 0.01 μg/l
Na2MoO4.2H2O 7 μg/l
NaHCO3 50 mg/l - Nominal and measured concentrations:
- Due to a mistake 100 instead of 140 mg test substance per litre was prepared as the highest concentration, therefore, after correction the highest concentration was 71 instead of 100 mg a.i./l
% water soluble fraction at a loading rate of 71mg act.ingr./l: 1, 3.2, 10, 32, 100
average exposure concentratin mg TOC/l: 0.08, 0.1, 1.22, 13.37, 27.50 - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 23.09 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: % water soluble fraction prepared at a loading rate of 71mg act.ingr./l
- Basis for effect:
- growth rate
- Remarks on result:
- other: lower 95%-cl: EC50=17.08mg/l; upper 95%-cl: EC50=31.41mg/l
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.32 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- other: average exposure concentrations (mg TOC/l)
- Basis for effect:
- growth rate
- Remarks on result:
- other: lower 95%-cl: EC50=4.24mg/l; upper 95%-cl: EC50=6.58mg/l
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 7.78 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: % water soluble fraction prepared at a loading rate of 71mg act.ingr./l
- Basis for effect:
- biomass
- Remarks on result:
- other: lower 95%-cl: EC50=7.78mg/l; upper 95%-cl: EC50=8.56mg/l
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.65 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- other: average exposure concentrations (mg TOC/l)
- Basis for effect:
- biomass
- Remarks on result:
- other: lower 95%-cl: EC50=0.53mg/l; upper 95%-cl: EC50=0.77mg/l
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The substance and its decomposition products inhibit growth rate and biomass of Algae.
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