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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Study was conducted to access the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The test substance was insoluble in water. Therefore, the test solution was prepared by dissolving 3 mg, 6 mg, 12mg, 24 mg & 48 mg of the test substance in 2 liters of potable water (passed through reverse osmosis system) with continuous stirring for 48 hrs and after the completion of 48 hrs stirring filtration was done by Whatman filter paper manually to obtain the test concentrations of 1.5 mg/L, 3 mg/L, 6 mg/L, 12 mg/L & 24 mg/L, respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Animal species: Zebra Fish (Danio rerio)
Age of the animals: 3-4 months
Body length: average 2.7 cm
Body weight: 0.233 g (average)

Duration of housing: 15 days
Photoperiod: 12 hours light and 12 hours darkness
Oxygen content: 6.7 (mg/l)
pH: 6.9
Water temperature: 28 °C
Mortality (%) : During last 14 days = no mortality was observed
Diet: Standard brand feed, once daily


Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
42.5 mg of CaCO3
Test temperature:
23-25°C
Nominal and measured concentrations:
1.5 mg/L, 3 mg/L, 6 mg/L, 12 mg/L & 24 mg/L, respectively
Details on test conditions:
TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light and 12-hour darkness

Reference substance (positive control):
no
Key result
Duration:
24 h
Dose descriptor:
LL50
Effect conc.:
24 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Other details not known
Duration:
96 h
Dose descriptor:
LL0
Effect conc.:
3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Other details not known

8.1. Mortality and symptoms:

Nominal conc. (mg/l)

Number of fish

Mortality (cumulated) after

24h

48h

72h

96h

0 (control)

8

-

-

-

-

1.5

8

-

-

-

-

3

8

-

-

-

-

6

8

1

-

-

-

12

8

-

1

-

-

24

8

4

-

-

-

 

Visible Symptoms: Fish were moving slowly as compared to the control

 

Nominal conc. (mg/l)

Number of fish

Symptoms after

24h

48h

72h

96h

0 (control)

8

NS8

NS8

NS8

NS8

1.5

8

M8

M8

M8

M8

3

8

M8

M8

M8

M8

6

8

M7

M7

M7

M7

12

8

M8

M7

M7

M7

24

8

NM4

NM4

NM4

NM4

 

 


Explanation of symptoms:

A: apathy

N: narcotic-like state

C: swimming near water surface

D: swimming at the bottom of bowl

M: Slow movements compared to control

NS: No abnormal symptoms

Number behind symbol for symptom= number of affected fish

8.2. pH and oxygen content:

Nominal conc. (mg/l)

pH readings after

24h

48h

72h

96h

0 (control)

5.05

6.6

6.7

6.4

1.5

6.9

6.9

6.7

6.8

3

6.8

6.9

6.7

6.8

6

6.7

6.8

6.7

6.7

12

6.7

6.9

6.7

6.7

24

6.6

5.0

6.7

6.6


Nominal conc. (mg/l)

Oxygen content (mg/l) after

24h

48h

72h

96h

0 (control)

5.31

6.36

6.32

6.99

1.5

5.0

6.28

6.19

6.80

3

4.96

5.93

6.10

6.56

6

4.69

5.22

5.61

6.02

12

4.58

5.90

6.17

6.49

24

3.80

5.68

6.13

5.95

 

8.3. Test temperature

Nominal conc. (mg/l)

Temperature (°C) after

24h

48h

72h

96h

0 (control)

24

23

25

24

1.5

24

23

25

24

3

24

23

25

24

6

24

23

25

24

12

24

23

25

24

24

24

23

25

24

Validity criteria fulfilled:
yes
Conclusions:
After 24 hours of exposure to test item to various nominal test concentrations, LL50 was determine to be 24 mg/l and LL0 was at 3 mg/l. Based on the LL50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.
Executive summary:

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was insoluble in water. Therefore, the test solution was prepared by dissolving 3 mg, 6 mg, 12mg, 24 mg & 48 mg of the test substance in 2 liters of potable water (passed through reverse osmosis system) with continuous stirring for 48 hrs and after the completion of 48 hrs stirring filtration was done by Whatman filter paper manually to obtain the test concentrations of 1.5 mg/L, 3 mg/L, 6 mg/L, 12 mg/L & 24 mg/L, respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 24 hours of exposure to test item to various nominal test concentrations, LL50 was determine to be 24 mg/l and LL0 was at 3 mg/l. Based on the LL50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

Description of key information

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was insoluble in water. Therefore, the test solution was prepared by dissolving 3 mg, 6 mg, 12mg, 24 mg & 48 mg of the test substance in 2 liters of potable water (passed through reverse osmosis system) with continuous stirring for 48 hrs and after the completion of 48 hrs stirring filtration was done by Whatman filter paper manually to obtain the test concentrations of 1.5 mg/L, 3 mg/L, 6 mg/L, 12 mg/L & 24 mg/L, respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 24 hours of exposure to test item to various nominal test concentrations, LL50 was determine to be 24 mg/l and LL0 was at 3 mg/l. Based on the LL50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

Key value for chemical safety assessment

LC50 for freshwater fish:
24 mg/L

Additional information

Data available for the test chemical from various sources, study has been reviewed to determine the short term toxicity on fish of the test chemical. The studies are as mentioned below:

 

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was insoluble in water. Therefore, the test solution was prepared by dissolving 3 mg, 6 mg, 12mg, 24 mg & 48 mg of the test substance in 2 liters of potable water (passed through reverse osmosis system) with continuous stirring for 48 hrs and after the completion of 48 hrs stirring filtration was done by Whatman filter paper manually to obtain the test concentrations of 1.5 mg/L, 3 mg/L, 6 mg/L, 12 mg/L & 24 mg/L, respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 24 hours of exposure to test item to various nominal test concentrations, LL50 was determine to be 24 mg/l and LL0 was at 3 mg/l. Based on the LL50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

 

First study was supported by the second data from authoritative database. Study was designed to assess the effect of test chemical on the mortality of fish. Test conducted for 96 hrs. The lethal concentration (LC50) value of test chemical in fish in a 96 hr study on the basis of mortality effect was determine to be 5.6 mg/L. Thus considering the CLP Criteria for aquatic classification of the substance, it is concluded that test chemical exhibit short term toxicity to fish (Brachydanio rerio) in aquatic chronic 2 category.

 

Similar short term toxicity of test chemical were studied on the mortality of fish. The lethal concentration LC0, LC50 and LC 100 value of test chemical in fish (Brachydanio rerio) in a 96 hr study on mortality effect was determine to be 1,3.2 and 10 mg/L. Though the LC 50 value was assessed to be. Thus considering the CLP Criteria for aquatic classification of the substance, it is concluded that test chemical exhibit short term toxicity to fish (Brachydanio rerio) in aquatic chronic 2 category.

Thus based on the above data available from various sources, chemical consider to be toxic and classified as aquatic chronic 3 as per the CLP classification criteria.