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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From February 20 to March 26, 2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Batch number: CN0191205
Purity: 98.3% - Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken for analysis from the freshly prepared solutions at 0 hour and the old solutions at 48 hours.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 0.0503 g test item was weighed and dissolved in 500 mL of test water by magnetic stirring for 24 hours. After magnetic stirring, undissolved test item was observed in the solution, the solution was filtered through a 0.45 μm filter membrane and the filtrated solution was used to dilute other test solutions. Pipette 25.0, 40.0, 67.5, 107.5 and 175.0 mL of the filtrated solution and make up to 250 mL with test water. The solutions were mixed thoroughly, resulting in test solutions at 10%, 16%, 27%, 43% and 70% of the saturated solution prepared at 100 mg/L. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Age at study initiation (mean and range, SD): less than 24 hours old and not the first brood progeny.
- Source: Daphnia of stock culture was originally obtained from Applied Chemistry Department Pesticide Environmental Evaluation Laboratory of China Agricultural University. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 140 to 250 mg/L (as CaCO3)
- Test temperature:
- 18~22oC
- pH:
- 6.0 to 8.5
- Dissolved oxygen:
- not less than 3 mg/L during the test period
- Nominal and measured concentrations:
- nominal: 10%, 16%, 27%, 43% and 70% of the saturated solution prepared at 100 mg/L;
first definitive test: 2.38, 3.95, 6.92, 11.5 and 19.3 mg/L
second definitive test: 4.74, 7.31, 11.2, 17.1 and 25.4 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 200 mL glass beakers
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume:
- Volume of solution: 50 mL per vessel
- Aeration: Before usage, the water was aerated for more than 24 hours to attain dissolved oxygen saturation.
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 5 daphnia
- No. of vessels per concentration (replicates): 4 replicates
- No. of vessels per control (replicates): 4 replicates
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: the dilution water was tap water after being treated with Aquapro Pure Water System
- Total organic carbon: < 2 mg/L
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hr light: 8 hr dark
- Light intensity:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Biological observation: Observation intervals: 24 and 48 hours after exposure
VEHICLE CONTROL PERFORMED: yes
RANGE-FINDING STUDY
- Test concentrations: 1%, 5%, 10% and 50% of the saturated solution prepared at 100 mg/L
- Results used to determine the conditions for the definitive study: During the period of 48 hours, no immobilization or any toxic signs were observed in the control group and the test groups of 1%, 5% and 10% of 100 mg/L. 100% immobilization occurred in the test groups of 50% and 100% of 100 mg/L after 48 hours. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 23.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- During the 48-hour exposure period of the first definitive test, no immobilization and abnormal behavior or appearance was observed in the control group and all test groups.
During the 48-hour exposure period of the second definitive test, no immobilization and abnormal behavior or appearance was observed in the control group and the test groups of 20%, 30%, 45%, 67% of the saturated solution prepared at 100 mg/L, the daphnids exposed to the test group of 100% of the saturated solution prepared at 100 mg/L showed abnormal behavior such as hypoactivity and immobilization. - Results with reference substance (positive control):
- EC50-24 h of potassium dichromate to Daphnia magna was 1.44 mg/L, and the 95% confidence limits was 1.23 ~ 1.69 mg/L. The results met the recommended criterion in OECD Guideline No. 202: EC50-24 h of potassium dichromate is within the range of 0.6 mg/L to 2.1 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hour EC50 of the test item PF-06452787 (BOC Ketone) to Daphnia magna was determined to be 23.8 mg/L.
- Executive summary:
A Daphnia acute immobilisation test of PF-06452787 (BOC Ketone) was conducted with Daphnia magna over a 48-hour exposure period.
The 48-hour EC50 of the test item PF-06452787 (BOC Ketone) to Daphnia magna was determined to be 23.8 mg/L.
Reference
Description of key information
A Daphnia acute immobilisation test of PF-06452787 (BOC Ketone) was conducted with Daphnia magna over a 48-hour exposure period.
The 48-hour EC50 of the test item PF-06452787 (BOC Ketone) to Daphnia magna was determined to be 23.8 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 23.8 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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