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Reaction mass of trisodium [m[7-[[4'-[[6-benzamido-1-hydroxy-3-sulpho-2-naphthyl]azo]-3,3'-dihydroxy[1,1'-biphenyl]-4-yl]azo]-8-hydroxynaphthalene-1,6-disulphonato(7-)]]dicuprate(3-) and trisodium [m[4-[[4'-[[6-benzamido-1-hydroxy-3-sulpho-2-naphthyl]azo]-3,3'-dihydroxy[1,1'-biphenyl]-4-yl]azo]-3-hydroxynaphthalene-2,7-disulphonato(7-)]]dicuprate(3-)
EC number: 947-266-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From October 10th to November 07th, 1985
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Version / remarks:
- September 1982
- GLP compliance:
- yes
- Specific details on test material used for the study:
- The test solutions were prepared by adding the test material directly in the test water.
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source: ARA Rhein, Pratteln, Switzerland.
- Pretreatment: the sludge was centrifuged and washed three times with drinking water.
- Preparation of inoculum for exposure: the sludge was suspended in water with a solids content of 4 g/l and - after the addition of 20 ml synthetic sewage per litre sludge suspension - aerated overnight at 20 °C. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 180 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Test vessels: 4 litres glass beaker with 2 litre test solution.
- Aeration: air flow 0.5 to 1 l/min.
- Light: diffuse light.
- Sludge: 1 g/l
- Temperature: 22 ± 3 °C
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: drinking water.
- Total hardness: about 180 mg CaCO3/l.
- pH: 7.6 to 8.0. - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 725 mg/l, corresponding to 415 mg DOC/l
- Parameter:
- % degradation (DOC removal)
- Value:
- 57
- Sampling time:
- 3 h
- Parameter:
- % degradation (DOC removal)
- Value:
- 86
- Sampling time:
- 28 d
- Results with reference substance:
- DOC elimination > 90 % after 5 days.
- Interpretation of results:
- other: most of the test item resulted to be efficiently removed after 5 days
- Conclusions:
- Based on these results, most of the test item resulted to be efficiently removed after 5 days.
- Executive summary:
The biodegradability potential of test item exposed to activated sludge, domestic, non-adapted, was investigated under aerobic static conditions. The procedures outlined into the OECD guideline 302 B were followed.
Based on the data of the individual DOC determinations the test item reached a bioelimination of 86 % alter 5 days. The main elimination process occurred during the first days; in the first 3 hours the 57 % of the initial DOC resulted to be removed. Maximum elimination was attained after 5 days of incubation. Some elimination of the test substance due to adsorption to the activated sludge, on the glass surface or other physico-chemical processes was found as determined by means of DOC measurements 3 h after the start of the test.
The positive control, sodium benzoate, showed a biodegradation percentage higher than 90 after 5 days of incubation, confirming suitability of inoculum and test conditions.
Conclusion
Based on these results, most of the test item resulted to be efficiently removed after 5 days.
Reference
DOC-elimination
Sampling time | Test material mg DOC/l* | Control mg DOC/l* | DOC net mg/l | DOC elimination (%) |
3 hours | 180 | 9 | 171 | 57 |
5 days | 67.5 | 6.5 | 61 | 84.5 |
6 days | 63 | 9.5 | 53.5 | 86.5 |
8 days | 64 | 5 | 59 | 85 |
12 days | 64.5 | 6.5 | 58 | 85.5 |
13 days | 61.5 | 6 | 55.5 | 86 |
15 days | 66 | 5 | 61 | 84.5 |
18 days | 62 | 3 | 59 | 85 |
20 days | 64 | 5.5 | 58.5 | 85.5 |
22 days | 65 | 5.5 | 59.5 | 85 |
25 days | 57.5 | 2 | 55.5 | 86 |
27 days | 54.5 | 2 | 52.5 | 87 |
28 days | 58 | 4.5 | 53.5 | 86.5 |
*mean values of duplicate determination
Description of key information
Neither readily nor inherently biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
The biodegradability potential of test item exposed to activated sludge, domestic, non-adapted, was investigated under aerobic static conditions. The procedures outlined into the OECD guideline 302 B were followed. Based on the data of the individual DOC determinations the test item reached a bioelimination of 86 % alter 5 days. The main elimination process occurred during the first days; in the first 3 hours the 57 % of the initial DOC resulted to be removed.
On the basis of the substance removal and on the basis the physicochemical properties of the substance, it is expected that most of the elimination of the test substance should be attributed to adsorption to the activated sludge, on the glass surface or other physico-chemical processes. Based on these results, most of the test item resulted to be efficiently removed after 5 days. However, it is probable that the test item was mainly removed by adsorption and not degraded.
The expectation is confirmed by the comparison with the results obtained into a MITI test conducted on the structural analogous Similar Substance 01; the read acorss approach can be considered as reliable and adequate to investigate the biodegradability potential of the test item. Details on the read across approach are given into the IUCLID section 13.
Similar Substance 01 was investigated for its inherent biodegradability in a manometric respirometry test, over 28 days. The oxygen consumption of the test item in the test media was in the normal range of the oxygen consumption in the inoculum controls. Consequently, the substance resulted to be not biodegradable under the conditions of the test within 28 days. No degradation of the test item occurred in the abiotic control under the conditions of the test.
In the toxicity control, containing both test item and the reference item sodium benzoate, no inhibitory effect on the biodegradation of the reference item was determined. Thus, had no inhibitory effect on the activity of activated sludge microorganisms at the tested concentration of 65 mg/l. In the procedure controls, the reference item (sodium benzoate) was degraded by an average of 73 % and 84 % by exposure days 7 and 14, respectively, thus confirming the suitability of the activated sludge.
In conclusion, Direct Blue 251 is expected to be neither readily nor inherently biodegradable.
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