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EC number: 620-365-5 | CAS number: 9016-72-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004/06/01 to 2004/07/21
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: JMAFF 12 Nousan No. 8147
- Version / remarks:
- 2000
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Version / remarks:
- 1996
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 72-2 (Aquatic Invertebrate Acute Toxicity Test)
- Version / remarks:
- 1982
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 1984 and corresponding revised draft proposal (2004)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Duplicate samples containing 20 mL of the freshly prepared test media have been taken at test initiation from all test levels and the controls prior to introducing the test animals. For additional stability measurements the aged replicates of each concentration level were pooled after 2 days in one vessel and samples have then be taken from this pooled media.
Samples, not intended for immediately measurement, have been stored deep frozen. - Vehicle:
- yes
- Remarks:
- Dimethylformamide (DMF)
- Details on test solutions:
- Initially a stock solution (stock solution A) was prepared by dissolving 2915.8 mg test item in 50 mL DMF (Dimethylformamide) (= 48g a.s./L). This solution was ultrasonified for 5 minutes, followed by 26 minutes of stirring.
From this solution, sub-dilutions were established:
Stock solution B >5 mL solution A + 5 mL DMF = 24 g test item/L
Stock solution C >5 mL solution B + 5 mL DMF = 12 g test item/L
Stock solution D > mL solution C + 5 mL DMF = 6.0 g test item/L
Stock solution E >5 mL solution D + 5 mL DMF = 3.0 g test item/L
Stock solution F >5 mL solution E + 5 mL DMF = 1.5 g test item/L
Each solution was stirred until visible homogeneity was achieved.
From each of these stock solutions 100 µL were taken and carefully added to an amount of 1000 mL test water in order to establish the exposure-concentrations. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- First instars (Daphnia magna) < 24 hours old, from laboratory stock breeding, were used for this study. The first instars used in the test were obtained by repeated careful screening of a breeding culture of defined age, using plastic screens with 0.6 and 0.2 mm mesh size less than 24 h before test initiation. The parent daphnids were of the same age (±12 hours), 21 days old and from the fourth brood or later. No males, ephippia or dead animals were present in the cultures. The cultures showed no delay in first brood.
6 x 5 animals per concentration - Test type:
- static
- Water media type:
- other: "M7 medium" as documented in the "Original Draft" of an EEC Daphnia magna Pilot Ring Test. The medium is prepared using deionised water by adding mineral salts. Immediately before use in a study, the vitamin components will be added.
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- After 24 and 48 hours, behaviour of the water fleas was visually evaluated.
- Hardness:
- 10° dH (= 178 mg/L CaCO3)
- Test temperature:
- 20.3 - 20.6°C
- pH:
- 7.9 - 8.1
- Dissolved oxygen:
- 8.6 - 8.8 O2 Conc. (mg/L)
- Conductivity:
- 576 µS/cm
- Nominal and measured concentrations:
- Nominal concentrations: 0.15, 0.30, 0.60, 1.20, 2.40 and 4.80 mg a.s./L, Mean measured concentrations: 0.152, 0.241, 0.498, 1.07, 2.18 and 4.04 mg a.s./L
- Details on test conditions:
- The water flea Daphnia magna was exposed to Propineb over a 48 hours period under static conditions. The test vessels consisted of 100 mL glass beakers, individually labelled and filled with 50 mL of the test solution, corresponding to a fluid level of approximately 3 cm height. Six vessels (replicates), each provided with five daphnids (= 10 mL test solution per daphnid), were used per treatment group and control (=30 animals per study group). The beakers were covered with transparent glass plates and placed in a climate controlled environment (isolated chamber) between 18 and 22°C (maximum allowed deviation ± 1°C within 48 hours). Lighting was adjusted to a 16:8 hours light dark cycle at about 1400 lux.
The water fleas were not fed and the test solutions were not artificially aerated during exposure. Any surface in contact with the test solution was made of glass or other chemical inert material. - Reference substance (positive control):
- no
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.66 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.5 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Based on mean-measured concentrations of propineb, the EC50 for immobilisation after 48 hours of static exposure was 1.50 mg a.s./L (95% confidence limits 1.28 -1.76 mg a.s./L).
The test was performed wit the formulated product Propineb VM 80. - Validity criteria fulfilled:
- yes
- Conclusions:
- The aim of the study was to determine the influence of propinep on mobility of Daphnia magna over 48 hours in a static exposure, expressed as EC50 for immobilisation.
First instars of Daphnia magna <24 h old were exposed to nominal concentrations of 0, 0.15, 0.30, 0.60, 1.20, 2.40 and 4.80 mg a.s./L. Based on mean-measured concentrations of propineb, the EC50 for immobilisation after 48 hours was determined to be 1.50 mg a.s./L (95% confidence limits 1.28 -1.76 mg a.s./L). - Executive summary:
The aim of the study was to determine the influence of propinep on mobility of Daphnia magna over 48 hours in a static exposure, expressed as EC50 for immobilisation. First instars of Daphnia magna <24 h old were exposed to nominal concentrations of 0, 0.15, 0.30, 0.60, 1.20, 2.40 and 4.80 mg a.s./L. Based on mean-measured concentrations of propineb, the EC50 for immobilisation after 48 hours was determined to be 1.50 mg a.s./L (95% confidence limits 1.28 -1.76 mg a.s./L).
Reference
Table 2
Toxicity to Daphnia magna (based on nominal concentrations of pure Propineb):
Test concentration | Exposed Daphnids (=100%) | Immobilised Daphnids | |||||
mg product/L | mg a.s./L | mg a.s./L | 24h | 48h | |||
Nominal | Nominal | Mean Measured | n | %±SD | n | %±SD | |
Contorol | 30 | 0 | 0 | 0 | 0 | ||
DMF Control | 30 | 0 | 0 | 0 | 0 | ||
0.18 | 0.15 | 0.15 | 30 | 0 | 0 | 0 | 0 |
0.36 | 0.30 | 0.24 | 30 | 0 | 0 | 0 | 0 |
0.73 | 0.60 | 0.50 | 30 | 0 | 0 | 0 | 0 |
1.46 | 1.20 | 1.07 | 30 | 0 | 0 | 7 | 23±27 |
2.91 | 2.40 | 2.18 | 30 | 5 | 17±15 | 26 | 87±16 |
5.83 | 4.80 | 4.04 | 30 | 9 | 30±11 | 28 | 93±10 |
*) DMF (0.1 mL/L dimethylformamide)
Table 3
Statistical Results of Probit Analysis Conducted for Determination of EC50 Values:
Probit Analysis for Data obtained after | Slope Function (after Litchfield & Wilcoxon) | EC 50 mg a.s./L mean meas. | lower 95% cl mg a.s./L mean meas. | upper 95% cl mg a.s./L mean meas. |
24 hours | 2.2 | 5.66 | 3.48 | n.d. |
48 hours | 1.6 | 1.50 | 1.28 | 1.76 |
n.d.: not determined due to mathematical reasons
Description of key information
The aim of the study was to determine the influence of propineb on the mobility of Daphnia magna over 48 hours in a static exposure, expressed as EC50 for immobilisation. The main guideline used for this study was OECD 202. Based on mean-measured concentrations of propineb, the 48-h EC50 for was determined to be 1.50 mg a.s./L (95 % confidence limits 1.28 -1.76 mg a.s./L).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Remarks:
- 48 hours
- Effect concentration:
- 1.5 mg/L
Additional information
Another study not included in the summary, was performed by Bruns 2010. The study was performed, to detect possible effects of the test item on mobility of Daphnia magna caused by 48 hours of exposure in a static laboratory test system. Daphnia magna (1st instars <24 h old were exposed to nominal concentrations of 0, 0.63, 1.25, 2.50, 5.00 and 10.0 mg form./L. No immobility or other effects on behaviour occurred in untreated control within 48 hours of exposure. Based on nominal concentrations of propineb formulation the 48-h EC50 was determined to be 2.85 mg a.s./L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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