Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 911-501-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-11-29 - 2018-03-22
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study according to guidelines
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- April 2004
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- March 2015
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 100 mg/L
- Sampling method: Control and test solution were sampled in dupblicate at the start of exposure and at 48h.
- Sample storage conditions before analysis: All samples were stored in amber glass bottles in the dark at a temperature of ≤ -18°C after sampling and before shipment. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
As the test item is poorly water-soluble, the following approach was used taking into account aspects of ISO 14442 (2006), Water quality -- Guidelines for algal growth inhibition tests with poorly soluble materials, volatile compounds, metals and waste water, and OECD 2000, Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures. OECD Series on Testing and Assessment Number 23. ENV/JM/MONO(2000)6.
A stock solution was prepared at a nominal loading rate of 100 mg/L as described below by adding 0.098 mL of the test item to 1000 mL of the test medium. This stock solution was stirred for 46 hours and 20 minutes at ambient temperature in the dark.
Thereafter this stock solution was filtered, using a glass fibre filter with an average retention capacity of 0.4 µm (MN GF-5). The initial 100 mL of the filtrate were used to rinse the collection vessel and were subsequently discarded.
Particles were visible on the water surface, so the stock solution was filtered once more with a new filter (0.4 µm; MN GF-5). The first 100 mL of the filtrate were used to rinse the collection vessel and were afterwards discarded. After the second filtration a minor number of undissolved particles were visible on the water surface. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: M10
- Source: Daphnia magna STRAUS (clone M10), a water flea, cultured at ECT Oekotoxikologie GmbH since December 22, 2011
- Age of parental stock: < 35 days
- Feeding during test: none - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 254 mg/L CaCO3
- Test temperature:
- 20.7 - 21.5 °C
- pH:
- 6.8 - 7.6
- Dissolved oxygen:
- 76 - 94 % of air saturation
6.6 - 8.3 mg/L - Conductivity:
- 665 µS/cm
- Nominal and measured concentrations:
- 0, 100 mg/L (nominal concentrations)
0, 31.7 mg/L (measured concentrations) - Details on test conditions:
- TEST SYSTEM
- Test vessel:100-mL glass beakers, closed by watch glass
- Material, size, headspace, fill volume: 50-60 mL fill volume
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Test medium: Elendt medium M4
OTHER TEST CONDITIONS
- Adjustment of pH: None
- Photoperiod: 16h light / 8h dark
- Light intensity: 388-564 lx - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 31.7 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 31.7 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities:
- Mortality of control: 0%
- Other adverse effects control: None
Validity criteria:
% of immoible daphnids in the control was less than 10% (0%) and dissolved oxygen concentration in control and test vessels at the end of the test were greater than 3 mg/L (> 8 mg/L) - Results with reference substance (positive control):
- 24h EC50 0.74 mg/L
The results of this reference test are acceptable and the test conditions are reliable and the sensitivity of the test system could be demonstrated. - Validity criteria fulfilled:
- yes
- Conclusions:
- In this limit test no immobile daphnids were observed in the control or in the limit concentration of 100% of a saturated solution at a loading rate of 100 mg test item.
- Executive summary:
The acute toxicity of the Reaction mass of dioctyl hydrogen phosphate and octyl dihydrogen phosphate to Daphnia magna was tested according to OECD guideline 202 and GLP conditions.
In this limit test no immobile daphnids were observed in the control or in the limit concentration of 100% of a saturated solution at a loading rate of 100 mg test item.
It is therefore concluded that the EC50of the test item is higher than the limit concentration tested (nominal:100% of a saturated solution at a loading rate of 100 mg test item). This corresponds to a geometric mean measured concentration of the main constituents (sum of mono- & diester) of 31.7 mg/L.
Reference
Table 1: Cumulative number of immobile Daphnia magna after 24 and 48 h
nominal concentration |
total number of introduced daphnids |
total number of immobile daphnids |
immobile daphnids (% of introduced) |
total number of immobile daphnids |
immobile daphnids (% of introduced) |
[% of filtrate]1 |
|
[24 h] |
[24 h] |
[48 h] |
[48 h] |
Control |
20 |
0 |
0.0 |
0 |
0.0 |
100 |
20 |
0 |
0.0 |
0 |
0.0 |
1% of filtrate of saturated solution at a loading rate of 100 mg/L
Table 2: Summary of measured concentrations of main constituents during the test
Nominal concentration |
Test period [h] |
Age of test solution [h] |
Measured concentration diester[mg/L] |
Measured concentration monoester[mg/L] |
Measured concentrationb)[mg/L] |
Geometric mean measured concentrationb)over 48 h [mg/L] |
0 (control) |
0 |
0 |
n.d. |
n.d. |
n.d. |
-- |
100 |
0 |
0 |
2.19 |
46.4 |
48.6 |
-- |
0 (control) |
48 |
48 |
n.d. |
n.d. |
n.d. |
n.a. |
100 |
48 |
48 |
0.78 |
19.9 |
20.7 |
31.7 |
a)Individual filtrate (WAF) at a loading rate of 100 mg/L
b)sum of main constituents diester + monoester)
Limit of Quantification (LOQ): 1.0 mg/L; n.d.: < 0.3 mg/L; n.a. = not applicable
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.