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EC number: 700-222-4 | CAS number: 1065519-44-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16.-18.03.2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP - Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23 (2000)
- GLP compliance:
- yes
Test material
- Reference substance name:
- N-(4-{[3-(dimethylamino)propyl]sulfamoyl}phenyl)-2-[(2-methoxy-4-nitrophenyl)diazenyl]-3-oxobutanamide
- EC Number:
- 700-222-4
- Cas Number:
- 1065519-44-9
- Molecular formula:
- C22H28N6O7S
- IUPAC Name:
- N-(4-{[3-(dimethylamino)propyl]sulfamoyl}phenyl)-2-[(2-methoxy-4-nitrophenyl)diazenyl]-3-oxobutanamide
- Details on test material:
- - Name of test material (as cited in study report): NO.408 YELLOW
- Physical state: powder
- Analytical purity: 98.6%
- Lot/batch No.: 081105-1
- Expiration date of the lot/batch: 04. November 2009
- Storage condition of test material: at room temperature in the dark
- Other: solubility in water: 0.38 mg/L
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Preparation of test solutions started with a stock solution prepared at a loading rate of 100 mg/L. A 15-minute treatment period with ultrasonic waves was followed by a magnetic stirring period of 45 h to accelerate the dissolving of the test substance in the test medium. This resulted in a solution that still ocntained some undissolved material, which was consequently removed by filtration through a 0.45 μm membrane filter (Whatman, RC55).
- Controls: Test medium without test substance or other additives.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus, 1820
- Source: In-house laboratory culture with a known history
- Age at study initiation (mean and range, SD): < 24 h
- Feeding during test: no
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 180 mg CaCO3/L
- Test temperature:
- 20.3 °C - 21.2 °C
- pH:
- see table below
- Dissolved oxygen:
- see table below
- Nominal and measured concentrations:
- Nominal concentrations: 1.0, 10 and 100% of a 0.45 μm filtered solution prepared at a loading rate of 100 mg/L
Measured concentrations: Analysis of the samples taken from the filtrate at the start of the combined limit/range-finding test showed a measure concentration of 0.256 mg/L. During the exposure period the measured concentration decreased to 0.113 mg/L. The average exposure concentration being the geometric mean was calculated to correspond to 0.17 mg/L. This concentration exceeded the maximum solubility that was limited to 19.8 μg/L. - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 100 mL all-glass test vessels containing 80 mL of test solution
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 (highest concentration) and 2 (remaining concentrations)
- No. of vessels per control (replicates): 4
OTHER TEST CONDITIONS
- Photoperiod: 16 h light, 8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility was monitored daily
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Justification for using less concentrations than requested by guideline: study is a combined limit/range-finding test - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.17 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: 0%
- Effect concentrations exceeding solubility of substance in test medium: The average exposure concentration of 0.17 mg/L exceeded the maximum solubility that was limited to 19.8 μg/L. - Results with reference substance (positive control):
- - EC50: 0.30 mg/L with a 95% confidence interval between 0.25 and 0.35 mg/L
Any other information on results incl. tables
Table 2: Acute immobilisation of daphnids after 24 and 48 hours in the combined limit/range-finding test
Test group NO.408 YELLOW |
Vessel number |
Number of Daphnia |
Response at 24 h |
Response at |
||
Number |
Total % |
Number |
Total % |
|||
Control |
A B C D |
5 5 5 5 |
0 0 0 0 |
0 |
0 0 0 0 |
0 |
1.0 |
A B |
5 5 |
0 0 |
0 |
0 0 |
0 |
10 |
A B |
5 5 |
0 0 |
0 |
0 0 |
0 |
100 |
A B C D |
5 5 5 5 |
0 0 0 0 |
0 |
1 1 1 1 |
20 |
1Average exposure concentration
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.